摘要
用于细胞学检查的支气管肺泡灌洗液(BALF)的制备方法对细胞定量结果有显著影响。研究表明,细胞离心导致低估淋巴细胞的数量和膜过滤器的制备,从而低估中性粒细胞的数量。作为这两种技术的一种简单的替代方法,BALF细胞可以通过显微镜载玻片涂片技术制备,这是一种熟悉的制备外周血鉴别计数的方法。为了比较载玻片法测定的细胞差异和细胞离心法测定的细胞差异,采用标准方法从35个BALF标本中分离细胞,并用血细胞计数仪进行计数。细胞离心3 min, 57 x g;cyspin 2)和5 × 10(5)细胞在5 microL显微镜载玻片涂片。两个样本风干后,使用May-Grunwald Giemsa染色,计数600个细胞以获得差异。为了检验载玻片涂片技术取样的充分性,在固定数量的BALF细胞中加入已知数量的淋巴细胞或中性粒细胞,制作载玻片,测定600个细胞的差异。将得到的微分与计算得到的微分进行比较。用载玻片涂片技术制备BALF细胞,细胞形态保存良好。 Compared to cytocentrifugation, microscope slide smear preparations had significantly higher percentages of lymphocytes. The microscope slide smears for the samples with predetermined numbers of cells yielded lymphocyte and neutrophil percentages which did not differ from the calculated differentials (59.6 +/- 1.5 vs 59.6 +/- 5.2% and 54.6 +/- 6.0 vs 53.1 +/- 6.0%, respectively). Varying the number of cells counted from 100 to 800 confirmed the reproducibility of the counts for counting 600 cells. Using 5 x 10(5), 2.5 x 10(5), or 1 x 10(5) cells per preparation demonstrated that adequate specimens could be obtained from as few as 1 x 10(5) cells. Thus, microscope slide smear preparation is a simple and accurate method for the quantitation of bronchoalveolar lavage fluid cytology.