JF -欧洲呼吸杂志JO - Eur Respir J SP - 603 LP - 608 DO - 10.1183/09031936.96.09030603 VL - 9 IS - 3 AU - Thompson, AB AU - Teschler, H AU - Wang, YM AU - Konietzko, N AU - Costabel，U Y1 - 1996/03/01 UR - //www.qdcxjkg.com/content/9/3/603.abstract N2 -用于细胞学检查的支气管肺泡灌洗液(BALF)的制备方法对细胞定量结果有显著影响。研究表明，细胞离心导致淋巴细胞数量低估，膜过滤制备导致中性粒细胞数量低估。作为这两种技术的简单替代，BALF细胞可以通过显微镜载玻片涂片技术制备，这是一种熟悉的用于制备外周血进行差异计数的方法。为了比较显微镜载玻片技术测定的细胞差异与细胞离心法测定的细胞差异，使用标准方法从35个BALF样本中分离细胞，并使用血细胞计进行计数。细胞离心(3 min, 57 x g;细胞旋2)和5 × 10(5)个细胞在5 microL显微镜玻片涂片。两种样品均风干，用May-Grunwald Giemsa染色，计数600个细胞以获得差异。为了检验玻片涂片技术取样的充分性，将已知数量的淋巴细胞或中性粒细胞添加到固定数量的BALF细胞中，制备玻片涂片，并对600个细胞进行鉴别。将得到的微分与计算的微分进行比较。 Preparation of BALF cells with the microscope slide smear technique yielded well-preserved cell morphology. Compared to cytocentrifugation, microscope slide smear preparations had significantly higher percentages of lymphocytes. The microscope slide smears for the samples with predetermined numbers of cells yielded lymphocyte and neutrophil percentages which did not differ from the calculated differentials (59.6 +/- 1.5 vs 59.6 +/- 5.2% and 54.6 +/- 6.0 vs 53.1 +/- 6.0%, respectively). Varying the number of cells counted from 100 to 800 confirmed the reproducibility of the counts for counting 600 cells. Using 5 x 10(5), 2.5 x 10(5), or 1 x 10(5) cells per preparation demonstrated that adequate specimens could be obtained from as few as 1 x 10(5) cells. Thus, microscope slide smear preparation is a simple and accurate method for the quantitation of bronchoalveolar lavage fluid cytology. ER -