@article {Thompson603, author = {Thompson, AB and Teschler, H and Wang, YM and Konietzko, N and Costabel, U}， title ={用显微镜玻片涂片制备支气管肺泡灌洗液}，volume = {9}， number = {3}， pages ={603—608}，year = {1996}， publisher = {European Respiratory Society}，188bet官网地址用于细胞学检查的支气管肺泡灌洗液(BALF)的制备方法对细胞定量结果有显著影响。研究表明，细胞离心导致对淋巴细胞数量的低估，膜过滤器制备导致对中性粒细胞数量的低估。作为这两种技术的一种简单替代，BALF细胞可以通过显微镜玻片涂片技术制备，这是为鉴别计数准备外周血的常见手段。为了比较显微镜载玻片技术测定的细胞差异与细胞离心测定的细胞差异，采用标准方法从35个BALF样本中分离细胞，并用血细胞计计数。细胞离心3分钟，57 × g;细胞旋2)和5 × 10(5)细胞在5微l的显微镜玻片涂片。两个样本风干，May-Grunwald Giemsa染色，计数600个细胞以获得差异。为了检验显微镜玻片涂片技术取样的充分性，将已知数量的淋巴细胞或中性粒细胞加入固定数量的BALF细胞中，制备显微镜玻片涂片，并在600个细胞上测定差异。将得到的微分与计算得到的微分进行比较。 Preparation of BALF cells with the microscope slide smear technique yielded well-preserved cell morphology. Compared to cytocentrifugation, microscope slide smear preparations had significantly higher percentages of lymphocytes. The microscope slide smears for the samples with predetermined numbers of cells yielded lymphocyte and neutrophil percentages which did not differ from the calculated differentials (59.6 +/- 1.5 vs 59.6 +/- 5.2\% and 54.6 +/- 6.0 vs 53.1 +/- 6.0\%, respectively). Varying the number of cells counted from 100 to 800 confirmed the reproducibility of the counts for counting 600 cells. Using 5 x 10(5), 2.5 x 10(5), or 1 x 10(5) cells per preparation demonstrated that adequate specimens could be obtained from as few as 1 x 10(5) cells. Thus, microscope slide smear preparation is a simple and accurate method for the quantitation of bronchoalveolar lavage fluid cytology.}, issn = {0903-1936}, URL = {//www.qdcxjkg.com/content/9/3/603}, eprint = {//www.qdcxjkg.com/content/9/3/603.full.pdf}, journal = {European Respiratory Journal} }