TY -的T1 -制备支气管肺泡灌洗液与显微镜涂片JF -欧洲呼吸杂志》乔和J SP - 603 LP - 608欧元六世- 9 - 3 AU -汤普森,AB非盟- Teschler H AU -王,YM盟——都N盟——Costabel用于细胞学检查的支气管肺泡灌洗液(BALF)的制备方法可以显著影响细胞定量结果。研究表明，细胞离心导致淋巴细胞数量的低估，膜过滤制剂导致中性粒细胞数量的低估。作为这两种技术的一种简单的替代方法，BALF细胞可以通过显微镜玻片涂片技术制备，这是一种常见的制备外周血进行差异计数的方法。为了比较显微镜载玻片技术测定的细胞差异和细胞离心测定的细胞差异，使用标准方法从35份BALF样品中分离细胞，并使用血细胞计数仪计数。细胞离心(3 min, 57 x g;细胞自旋2)和5 × 10(5)细胞在5 microL显微镜涂片。两个样本均风干，使用May-Grunwald Giemsa染色，计数600个细胞以获得差异。为了测试显微镜涂片技术取样的充分性，在固定数量的BALF细胞中加入已知数量的淋巴细胞或中性粒细胞，制备显微镜涂片，并对600个细胞进行鉴别。得到的微分与计算的微分进行了比较。 Preparation of BALF cells with the microscope slide smear technique yielded well-preserved cell morphology. Compared to cytocentrifugation, microscope slide smear preparations had significantly higher percentages of lymphocytes. The microscope slide smears for the samples with predetermined numbers of cells yielded lymphocyte and neutrophil percentages which did not differ from the calculated differentials (59.6 +/- 1.5 vs 59.6 +/- 5.2% and 54.6 +/- 6.0 vs 53.1 +/- 6.0%, respectively). Varying the number of cells counted from 100 to 800 confirmed the reproducibility of the counts for counting 600 cells. Using 5 x 10(5), 2.5 x 10(5), or 1 x 10(5) cells per preparation demonstrated that adequate specimens could be obtained from as few as 1 x 10(5) cells. Thus, microscope slide smear preparation is a simple and accurate method for the quantitation of bronchoalveolar lavage fluid cytology. ER -