TY -的T1 -人类血液单核细胞,但不是肺泡巨噬细胞,揭示增加CD11b / CD18表达式和粘附性能在receptor-dependent激活JF -欧洲呼吸杂志》乔和J SP - 1188 LP - 1194欧元六世- 9 - 6盟Lundahl J盟——Hallden G AU - Skold教授,CM Y1 - 1996/06/01 UR - //www.qdcxjkg.com/content/9/6/1188.abstract N2 -β2整合素受体CD11b / CD18介导粘附到内皮细胞以及细胞外基质成分。本研究进行调查周边人类血液单核细胞(BMs)和肺泡巨噬细胞(AMs)对定量水平CD11b / CD18和粘附性能与激活的状态。百时美施贵宝和AMs是从健康受试者招募。定量分析的表面表达CD11b / CD18流仪技术和粘附性能albumin-coated表面进行休息和N-formyl-methionyl-leucyl-phenylalanine (fMLP)激活细胞。受体独立刺激(phorbol-12-myristate-13-acetate (PMA)和ionomycin)被用于额外的实验。细胞内储存CD11b / CD18被流式细胞仪和免疫荧光显微镜评估。The surface expression of CD11b/CD18 on resting BMs increased fivefold (p < 0.01) upon fMLP activation. On resting AMs, the surface expression of CD11b/CD18 was significantly higher (p < 0.01) compared to resting BMs but did not increase further upon activation with fMLP, PMA or ionomycin. In contrast to BMs, no evidence for an additional intracellular pool of CD11b/CD18 was found in AMs. The adherence of resting BMs did not significantly differ from the adherence of resting AMs. After fMLP activation, the adherence of BMs, but not AMs, increased significantly (p < 0.05). Our results indicate that in vivo differentiation of human blood monocytes into alveolar macrophages implies reduced responsiveness to fMLP in terms of CD11b/CD18 upregulation and adhesion properties, and that the lack of upregulation of CD11b/CD18 on alveolar macrophages presumably depends on the absence of an additional intracellular pool. ER -