@Article {Sadeghi-Hashjin286，作者= {Sadeghi-Hashjin，G和Henricks，PA和Folkerts，G和Verheyen，Ak和Van der Linde，HJ和Nijkamp，fp}，fp}，fp}，title = {bovine of bovine parthacheal permita in vietro：上皮和一氧化氮}，体积= {9}，number = {11}，页= {2286--2293}，年= {1996}，Publisher = {欧洲呼吸社会}，摘要= {airway Epitheplium periathium上188bet官网地址皮抑制性抑制因素，例如一氧化氮（NO）和前列腺素E2（PGE2），可能抵消支气管收缩。我们调查了上皮源性抑制性物质是否对体外牛气管反应产生至关重要的影响。比较了对完整的组胺和上皮性气管平滑肌条的响应的等渗和等距收缩。另外，L-精氨酸（L-ARG），N（G）-Nitro-L-精氨酸甲基埃斯特（L-NAME）和N（g） - 共甲基L-精氨酸（L-NMMA）的作用对组胺研究了响应能力。测量了从气管上皮中释放NO和PGE2。从气管平滑肌条中去除上皮并没有改变产生一半最大作用（PD2）或最大效应（EMAX）一半的组胺浓度的负模。将组织与L-ARG或L-NAME孵育25分钟不会影响基础张力或组胺引起的收缩。然而，与L-NMMA的孵育增加了基础张力，并导致组胺略有反应。 S-nitroso-N-acetyl-penicillamine (SNAP, a direct NO donor) reversed the contraction induced by histamine in a concentration-dependent manner. Stimulation of the epithelial layer by 0.1 microM histamine increased the release of NO 3-4 fold compared to basal levels; this effect was completely inhibited in the presence of L-NMMA. In addition, 1 mM histamine caused a significant increase in the release of PGE2 from the epithelial tissue. In conclusion, no functional inhibitory influence of the epithelium can be identified in bovine airways. The S-nitroso-N-acetyl-penicillamine-induced relaxation demonstrates the presence of a nitric oxide sensitive pathway in bovine airways. However, the amounts of nitric oxide and prostaglandin E2 released from bovine tracheal epithelium are probably too low to exert a significant effect on the histamine-induced contractions.}, issn = {0903-1936}, URL = {//www.qdcxjkg.com/content/9/11/2286}, eprint = {//www.qdcxjkg.com/content/9/11/2286.full.pdf}, journal = {European Respiratory Journal} }