RT期刊文章SR电子T1阿片类药物调节胆碱能收缩而不是nonadrenergic放松在豚鼠气道体外摩根富林明欧洲呼吸杂志乔和J FD欧洲呼吸学会SP 2280欧元OP 2285 10.1183 / 09031936.96.09112280 VO 9 11 A1 Pype, JL A1杜邦,LJ A1 Demedts, MG A1 Verleden,通用汽车年1996 UL http://erj.e188bet官网地址rsjournals.com/content/9/11/2280.abstract AB (D-ALa2、NMePhe4 Gly-ol5)脑髓苷(DAMGO),选择性mu-opioid受体激动剂,先前已证明抑制胆碱能和非胆碱能收缩豚鼠气道。相反，阿片类药物在8 Hz刺激下对气管上部的胆碱能神经传递没有抑制作用。我们研究了选择性mu-阿片受体激动剂DAMGO、选择性δ -阿片受体激动剂[D-Pen 2,5] encephalin (DPDPE)和选择性kappa-阿片受体激动剂U-69593在不同刺激频率下是否能调节气管上端胆碱能收缩。此外，我们还研究了DAMGO、DPDPE和U-69593是否也能调节iNANC松弛。DAMGO (1-100 microM)抑制气管上端胆碱能收缩，在1 Hz时最大抑制57+/-15% (n=4;p < 0.05)。另一方面，DPDPE (10 microM)和U69593 (10 microM)对胆碱能收缩无明显抑制作用。阿片受体拮抗剂纳洛酮(100微米)可拮抗DAMGO的抑制作用(n=5;p<0.01)对频率为2 Hz时胆碱能收缩的影响。 DAMGO (10 microM) did not displace the cumulative concentration-response relationship to acetylcholine (10 nM-10 mM), (n=4; NS). This provides evidence that prejunctional mu-opioid receptors (and not delta-opioid or kappa-opioid receptors) modulate cholinergic contraction in the upper trachea. In contrast, DAMGO (10 microM) had no significant inhibitory effect on the nonadrenergic relaxation (n=4; NS) in the upper trachea. Neither DPDPE nor U-69593 had any effect on the nonadrenergic relaxation. These findings suggest that DAMGO directly inhibits the cholinergic contraction and that the opioid receptor involved in the inhibition of the cholinergic contraction in the upper trachea is of the mu-opioid type. The finding that opioids inhibit cholinergic contraction without altering NANC relaxations suggests that distinct populations of nerves mediate these two effects.