TY -的T1 MCP-1分泌的肺不吸烟患者煤炭工人肺尘埃沉着病JF -欧洲呼吸杂志》乔和J SP - 557 LP - 562欧元六世- 10 - 3盟Boitelle AU -戈塞特,P盟——Copin MC盟——Vanhee D非盟-马奎特,CH盟——Wallaert B非盟,Gosselin B非盟- Tonnel AB Y1 - 1997/03/01 UR - //www.qdcxjkg.com/content/10/3/557.abstract N2 -接触煤尘的发展导致煤炭工人肺尘埃沉着病(CWP),疾病与巨噬细胞的积累下呼吸道。招聘机制控制单核细胞仍知之甚少。由于单核细胞化学引诱物蛋白1 (MCP-1)被认为是一个强有力的血液单核细胞的趋化现象的因素,我们分析了存在MCP-1 CWP患者的肺隔间。支气管肺泡灌洗液(BALF)从16不吸烟对照组27不吸烟的CWP患者(16使用简单的肺尘埃沉着病(SP)和11与进步的巨大纤维化(及)进行了分析。肺泡巨噬细胞(AMs)纯化的依从性和BALF被冻干集中十倍。MCP-1测量BALF和3 h是上层清液使用夹心酶联免疫吸附试验(ELISA)。MCP-1本地化的肺组织是由免疫组织化学组织部分从三个CWP患者和两个对照组。MCP-1 levels were significantly higher in concentrated BALF from patients with SP or PMF (median 370 and 555 pg x mL-1, respectively) than in those from control subjects (median 11 pg x mL-1) (p<0.001). Released MCP-1 in AM supernatants was enhanced in patients with CWP (median 83 pg x mL-1) but compared to controls (median 41 pg x mL-1) this level did not reach significance. Although significantly increased, AM counts in BALF from patients with CWP did not correlate with MCP-1 levels. MCP-1 levels in BALF correlated with MCP-1 levels in AM supernatants (p=0.47; p<0.02). In control lung specimens, MCP-1 was expressed by a few AMs, type II pneumocytes and perivascular smooth muscle cells. CWP sections were characterized by an increased number of AMs and mainly by the presence of fibroblasts (in the myogenic area of fibrotic lesions) and hyperplastic type II pneumocytes, which were strongly immunostained for MCP-1. Our data demonstrate that: 1) patients with coal worker's pneumoconiosis have a marked pulmonary overproduction of monocyte chemoattractant protein-1; and 2) in addition to alveolar macrophages, fibroblasts (probably myofibroblasts) and hyperplastic type II pneumocytes may also be responsible for this increased level of monocyte chemoattractant protein-1 in coal worker's pneumoconiosis. ER -