抽象
鼻病毒感染是哮喘恶化的主要原因。由于天然杀伤(NK)细胞是抗病毒先天反应的重要作用者,我们旨在评估NK细胞与严重哮喘患者的功能,以应对鼻病毒样分子或鼻病毒。
来自患有严重哮喘和健康供体患者的外周血单核细胞与病原体分子或鼻病毒(RV)-A9和RV-2刺激。分析了NK细胞活化,脱粒和干扰素(IFN)-γ表达。
NK cells from severe asthma patients were less cytotoxic than those from healthy donors in response to toll-like receptor (TLR)3, TLR7/8 or RV-A9 but not in response to RV-2 stimulation. Furthermore, when cultured with interleukin (IL)-12+IL-15, cytokines which are produced during viral infections, NK cells from patients with severe asthma were less cytotoxic and expressed less IFN-γ than NK cells from healthy donors. NK cells from severe asthmatics exhibited an exhausted phenotype, with an increased expression of the checkpoint molecule Tim-3.
Together, our findings indicate that the activation of NK cells from patients with severe asthma may be insufficient during some but not all respiratory infections. The exhausted phenotype may participate in NK cell impairment and aggravation of viral-induced asthma exacerbation in these patients.
抽象
来自严重哮喘患者的NK细胞表现出降低的活化,细胞毒性容量和IFN-γ生产后in vitrostimulation with rhinovirus RV-A9, in comparison to healthy donors. Exhausted phenotype is associated to increased Tim-3 expression.http://bit.ly/2ufjtdc.
脚注
本文提供了补充材料www.qdcxjkg.com
Conflict of interest: J. Devulder has a patent EP18306287.6 issued to INSERM.
Conflict of interest: C. Chenivesse has a patent EP18306287.6 issued to INSERM.
利益冲突:V.林德罗特无需披露。
利益冲突:S. Fry无需披露。
Conflict of interest: P-E. Lobert has nothing to disclose.
利益冲突:D. Hober无需披露。
利益冲突:A. Tsicopoulos没有什么可披露的。
Conflict of interest: C. Duez has a patent EP18306287.6 issued to INSERM.
支持声明:内部支持这项工作。J. Devulder由授权支持Ministèredel'enseignementsupérieur,de la Recherche et de L'Innovation,并通过补助金Fonds de Recherche enSanté呼吸们(FRSR). Funding information for this article has been deposited with theCrossref Funder Registry.
- 收到December 26, 2018.
- AcceptedFebruary 3, 2020.
- 复制right ©ERS 2020