补充材料
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补充材料ERJ-01214-2017_SUPPEMPEMT
Figure S1. Pulmonary arterial isolation procedure. Pulmonary arteries(*) were identified under a stereomicroscope due to their close vicinity to airways(§). Arteries were exposed by gradual removal of lung tissue until ~1-2cm of artery was visible. The artery was then removed en-bloc including side branches and forwarded to cell isolation. Scale bar represents 1 cm.ERJ-01214-2017_FIGURE_S1
Figure S2. Lung morphology in donor and IPAH samples. Representative images of sections derived from explanted lung samples (n=4). Magnified areas containing pulmonary vessels are also shown.ERJ-01214-2017_FIGURE_S2
图S3。在不同的流式细胞仪面板中测量的细胞群的相关图。a)总CD45+细胞,b)PMN:多形核中性粒细胞,c)CD3+ T细胞,d)Mac:巨噬细胞和E)肥大细胞。对于每个相关性,显示了从Spearman的等级相关性得出的P值和RHO(R)估计值。ERJ-01214-2017_FIGURE_S3
Figure S4. Correlation analysis of individual cell populations in matched lung samples. Analysed cell populations in the two separate pieces of lung were compared by Spearman's rank correlation. P values and rho (r) estimates derived from Spearman's rank correlation are shown.ERJ-01214-2017_FIGURE_S4
Figure S5. Abundance of cell populations excluded from global principal component analysis. Scatterplot and boxplot overlays of cell populations in IPAH and donor lungs as analysed by flow cytometry. NK, natural killer cells; NKT, natural killer T cells. Boxplots show median and interquartile range.ERJ-01214-2017_FIGURE_S5
Figure S6. Patient-to-patient variability in CD45+ cell populations. Heatmap representation presenting the patient-to-patient variability of the log odds ratio derived from the percentage CD45+ data for 18 individual cell populations. Blue colours indicate highest abundance, red lowest for a single cell type. PMN, polymorphonuclear neutrophils; Macs, Macrophages; Monos, monocytes; DC, dendritic cells; pDC, plasmacytoid DC; gd gamma delta.ERJ-01214-2017_FIGURE_S6
Figure S7. Euclidean clustering of individual IPAH and donor samples. Heatmap representation using Euclidean clustering of the log odds ratio derived from number of cells per mg tissue data for the 21 individual cell populations. Blue colours indicate highest abundance, red lowest for a single cell type. Monos, monocytes; DC dendritic cells, Macs, Macrophages; CTL, cytotoxic T cell; gd gamma delta; PMN, polymorphonuclear neutrophils.ERJ-01214-2017_FIGURE_S7
Figure S8. Abundance of remaining cell population in lung samples. Abundance of cell populations not included in图4如通过对IPAH和供体肺的流式细胞术分析确定。DC,树突状细胞;PMN,多形核中性粒细胞。框图显示中位数和四分位间范围,组之间的统计显着性是由Wilcoxon等级总和测试确定的。ERJ-01214-2017_FIGURE_S8
Figure S9. Relative proportions of CD45+ cells in lung samples. The relative proportions of multiple cell populations in IPAH and donor lungs. DC, dendritic cells; pDC; plasmacytoid dendritic cells; PMN, polymorphonuclear neutrophils. Boxplots show median and interquartile range, statistical significance between groups was determined by the Wilcoxon rank sum test.ERJ-01214-2017_FIGURE_S9
图S10。分离的肺动脉中的细胞群丰度。剩余的细胞群不包括图5以在IPAH流仪分析d donor samples. DC, dendritic cells; PMN, polymorphonuclear neutrophils. Boxplots show median and interquartile range, statistical significance between groups was determined by the Wilcoxon rank sum test.ERJ-01214-2017_FIGURE_S10