抽象的
血液 - 空气屏障是肺的最重要的功能元素,但是关于构成体内屏障的细胞的信息很少。本研究的目的是创造血液空气屏障的体外模型,这将允许调查细胞相互作用和肺泡代谢,并且适用于体外药物筛选。在微孔聚碳酸酯过滤器的相对侧生长大鼠肺泡和牛微血管内皮细胞,作为浸渍,灌注和液体 - 空气界面(LAI)培养物。光和透射电子显微镜检查培养条件对细胞形态的影响。对于浸渍和灌注共培养物,两个隔室都配有培养基。相比之下,对于液体空气界面研究,仅使用无血清培养基连续供应内皮细胞隔室,而II型肺细胞用空气通风。使用浸泡或灌注共培养时,肺细胞失去了形态学特性。在液体空气界面条件下,与完整的血管屏障相比,它们保留了其特征形态特征。初级II型肺细胞的子集保留其分化的表型,具有立方体形态,层状体和顶端微绒毛。这些II型肺细胞似乎通过紧密的连接连接到表达I型肺细胞的形态特征的细胞连接。 As shown herein, the liquid-air interface co-culture possesses many morphological characteristics of the intact blood-air barrier. In summary, this article describes the design of an artificial blood-air barrier, in which rat pneumocytes were cultivated with bovine microvascular endothelial lung cells on opposing sides of a microporous polycarbonate filter. We conclude that it might be a promising in vitro model for studies of molecular transport via the blood-air barrier, the investigation of repair mechanisms after alveolar injury, or as an in vitro screening system.