TY -的T1 Co-cultivation老鼠pneumocytes和牛内皮细胞的液态空气界面JF -欧洲呼吸杂志》乔和J SP - 968 LP - 975欧元六世- 9 - 5盟Gueven N盟——Glatthaar B盟——让HG盟——HaemmerleN2 -血-气屏障是肺中最重要的功能成分，但关于在体内构成这一屏障的细胞的信息很少。本研究的目的是建立一个血液-空气屏障的体外模型，用于研究细胞相互作用和肺泡代谢，并将适用于体外药物筛选。大鼠肺细胞和牛微血管内皮细胞分别在微孔聚碳酸酯过滤器的相对两侧进行浸泡、灌注和液-气界面(LAI)培养。采用光镜和透射电镜观察培养条件对细胞形态的影响。浸泡和灌注共培养时，两个室均提供培养基。相比之下，在液-气界面研究中，只有内皮细胞室持续提供无血清培养基，而II型肺细胞通气。在浸泡或灌注共培养时，肺细胞丧失形态学特征。在液-气界面条件下，与完整的血-气屏障相比，它们保留了大部分典型的形态特征。初级II型肺细胞亚群保持其分化表型，呈立方形态、板层体和顶端微绒毛。 These type II pneumocytes appeared to be connected by tight junctions to cells expressing morphological characteristics of type I pneumocytes. As shown herein, the liquid-air interface co-culture possesses many morphological characteristics of the intact blood-air barrier. In summary, this article describes the design of an artificial blood-air barrier, in which rat pneumocytes were cultivated with bovine microvascular endothelial lung cells on opposing sides of a microporous polycarbonate filter. We conclude that it might be a promising in vitro model for studies of molecular transport via the blood-air barrier, the investigation of repair mechanisms after alveolar injury, or as an in vitro screening system. ER -