抽象的
我们通过Phorbol Myristate醋酸酯(PMA)检测了抗氧化Lazaroid U-74389F对兔急性肺损伤的影响。接收U-74389F(15mg.kg-1 I.v.)或U-74389F载体的30分钟后,给予兔子(n = 60)PMA(60微克kg-1 i.v.)。PMA车辆注入兔子(n = 20)用作对照。PMA或PMA车辆注射后的5小时内,我们测量动脉pH,动脉氧张力(PA,O2),动脉二氧化碳张力(PA,CO2),以及中性粒细胞化学抑制剂白细胞介素-8的血浆浓度(IL-8)。在PostMorterem,检查肺部损伤并在组织学上检查。羟基醛水平在肺组织中测定为脂质过氧化指数。在支气管肺泡灌洗(BAL)中,测量总和差异细胞计数,蛋白质和IL-8浓度。与正常对照相比,用PMA挑战PMA挑战动脉酸中毒,Hypercapnia和低氧血症,伴随着血浆IL-8浓度的显着增加。U-74389F预处理的动物没有产生显着的动脉血气体异常,并在血浆中显着降低IL-8浓度。U-74389F未防止PMA诱导的脂质过氧化。 However, macroscopic signs of lung injury and the degree of alveolar haemorrhage and protein extravasation were significantly less severe in pretreated rabbits than in those given PMA alone. In addition, U-74389F significantly reduced IL-8 concentration and neutrophil number in BAL. By histological assessment, 80% of lung neutrophils were localized in alveolar spaces of animals receiving PMA alone. Conversely, in U-74389F pretreated animals, 75% of neutrophils were distributed within extra-alveolar blood vessels and alveolar septa. We conclude that lazaroid U-74389F attenuates lung injury in rabbits given PMA by preventing neutrophil migration into pulmonary alveoli. This effect may, in part, be related to downregulation of IL-8 production.