TY - JOUR T1 - Lazaroid U-74389F减轻佛波酯诱导的兔肺损伤JF -欧洲呼吸杂志JO - Eur Respir J SP - 758 LP - 764 DO - 10.1183/09031936.96.09040758 VL - 9 IS - 4 AU - Miniati, M AU - Cocci, F AU - Monti, S AU - Filippi, E AU - Sarnelli, R AU - Ferdeghini, M AU - Gattai, V AU - Pistolesi，M Y1 - 1996/04/01 UR - //www.qdcxjkg.com/content/9/4/758.abstract N2 -我们研究了抗氧化剂lazaroid U-74389F对肉豆蔻酸磷蓬醋酸酯(PMA)致兔急性肺损伤的影响。服用U-74389F(15毫克)后30分钟。kg-1 i.v.)或U-74389F灌胃，各组家兔(n = 60)给予PMA(60微克)。公斤注射)。PMA灌胃剂注射家兔(n = 20)作为对照。在PMA或PMA载体注射后5小时内，我们测量了动脉pH值、动脉氧张力(Pa,O2)、动脉二氧化碳张力(Pa,CO2)和中性粒细胞趋化剂白介素-8 (IL-8)的血浆浓度。在死后，检查肺的宏观损伤和组织学检查。丙二醛水平测定肺组织作为脂质过氧化的指标。在支气管肺泡灌洗(BAL)中，测量总细胞计数和差异细胞计数、蛋白质和IL-8浓度。与正常对照组相比，仅用PMA刺激的家兔出现动脉酸中毒、高碳酸血症和低氧血症，同时血浆IL-8浓度显著升高。 U-74389F pretreated animals did not develop significant arterial blood gas abnormalities and had significantly lower IL-8 concentration in plasma. U-74389F did not prevent PMA-induced lipid peroxidation. However, macroscopic signs of lung injury and the degree of alveolar haemorrhage and protein extravasation were significantly less severe in pretreated rabbits than in those given PMA alone. In addition, U-74389F significantly reduced IL-8 concentration and neutrophil number in BAL. By histological assessment, 80% of lung neutrophils were localized in alveolar spaces of animals receiving PMA alone. Conversely, in U-74389F pretreated animals, 75% of neutrophils were distributed within extra-alveolar blood vessels and alveolar septa. We conclude that lazaroid U-74389F attenuates lung injury in rabbits given PMA by preventing neutrophil migration into pulmonary alveoli. This effect may, in part, be related to downregulation of IL-8 production. ER -