抽象
痰由雾化高渗盐水吸入引起越来越多地用于监测哮喘的呼吸道炎症。本研究的目的是评估由痰诱导患者既轻,中度至重度哮喘获得测量整个痰样品中的炎症细胞和可溶性的标记的可重复性。12例轻度,变应性哮喘没有吸入性类固醇治疗和九例中度至重度,吸入类固醇治疗变应性哮喘研究了两个独立的天间隔至少2天。整个痰样本,由高渗(4.5%)盐水吸入诱导,均化,并分析细胞分类计数和白蛋白,纤维蛋白原浓度,白介素-8(IL-8),和嗜酸细胞阳离子蛋白(ECP)。重复性表示为组内相关系数(RI),并且如在百分比细胞重复性(CR)的系数或倍增中浓度。从两个轻度哮喘患者样品中含有80%以上的鳞状细胞和从分析中排除。对于两组组合式电池分类计数可重复性:嗜中性粒细胞,RI = 0.57和CR = 31.0;嗜酸性粒细胞,RI = 0.85,CR = 12.4;和淋巴细胞,RI = 0.76和CR = 6.9。流体相测量的可重复性是:对于白蛋白,RI = 0.71和CR = 3.2; for fibrinogen, Ri = 0.88 and CR = 2.8; for IL-8, Ri = 0.66 and CR = 2.2; and for ECP, Ri = 0.82 and CR = 1.1. We conclude that the repeatability of cellular and soluble markers of inflammation in induced sputum from patients with mild and moderate-to-severe asthma is satisfactory. Hence, induced sputum, processed by using the whole expectorated sample, seems to be a valuable method to monitor airway inflammation in asthma.