哮喘患者诱导痰中细胞和可溶性炎症标志物的重复性研究[J] 1996 %R 10.1183/09031936.96.09122441 %P 2441-2447 %V 9% N 12% X吸入雾化高渗盐水诱导痰越来越多地用于哮喘气道炎症监测。本研究的目的是评估通过诱导轻度和中度至重度哮喘患者的全痰样本中测量炎症细胞和可溶性标志物的可重复性。12例轻度特应性哮喘患者未接受吸入性类固醇治疗，9例中度至重度特应性哮喘患者接受吸入性类固醇治疗，研究时间分别为两天，间隔至少2天。吸入高渗盐水(4.5%)诱导的整个痰样本均质化，并分析差异细胞计数和白蛋白、纤维蛋白原、白细胞介素-8 (IL-8)和嗜酸性粒细胞阳离子蛋白(ECP)的浓度。重复性用类内相关系数(Ri)表示，用百分比细胞重复性系数(CR)或加倍浓度重复性系数表示。两名轻度哮喘患者的样本中含有超过80%的鳞状细胞，因此被排除在分析之外。两组细胞差异计数的可重复性联合为:中性粒细胞Ri = 0.57, CR = 31.0;嗜酸性粒细胞Ri = 0.85, CR = 12.4;淋巴细胞Ri = 0.76, CR = 6.9。流体相测量的重复性为:白蛋白，Ri = 0.71, CR = 3.2; for fibrinogen, Ri = 0.88 and CR = 2.8; for IL-8, Ri = 0.66 and CR = 2.2; and for ECP, Ri = 0.82 and CR = 1.1. We conclude that the repeatability of cellular and soluble markers of inflammation in induced sputum from patients with mild and moderate-to-severe asthma is satisfactory. Hence, induced sputum, processed by using the whole expectorated sample, seems to be a valuable method to monitor airway inflammation in asthma. %U //www.qdcxjkg.com/content/erj/9/12/2441.full.pdf