RT杂志文章SR电子T1哮喘患者诱导痰中炎症细胞和可溶性标志物的可重复性JF欧洲呼吸杂志JO Eur Respir J FD欧洲呼吸学会SP 2441 OP 2447 VO 9 IS 12 A1 in 't Veen, JC A1 de Gouw, HW A1 Smits, HH A1 Sont, JK A1 Hiemstra, PS A1 Sterk, PJ A1 Bel，188bet官网地址EH YR 1996 UL //www.qdcxjkg.com/content/9/12/2441.abstract AB吸入雾化高渗盐水引起的痰越来越多地用于监测哮喘气道炎症。本研究的目的是评估通过诱导轻度和中度至重度哮喘患者的全痰样本中测量炎症细胞和可溶性标志物的可重复性。12例轻度特应性哮喘患者未接受吸入性类固醇治疗，9例中度至重度特应性哮喘患者接受吸入性类固醇治疗，研究时间分别为两天，间隔至少2天。吸入高渗盐水(4.5%)诱导的整个痰样本均质化，并分析差异细胞计数和白蛋白、纤维蛋白原、白细胞介素-8 (IL-8)和嗜酸性粒细胞阳离子蛋白(ECP)的浓度。重复性用类内相关系数(Ri)表示，用百分比细胞重复性系数(CR)或加倍浓度重复性系数表示。两名轻度哮喘患者的样本中含有超过80%的鳞状细胞，因此被排除在分析之外。两组细胞差异计数的可重复性联合为:中性粒细胞Ri = 0.57, CR = 31.0;嗜酸性粒细胞Ri = 0.85, CR = 12.4;淋巴细胞Ri = 0.76, CR = 6.9。 The repeatability of the fluid phase measurements was: for albumin, Ri = 0.71 and CR = 3.2; for fibrinogen, Ri = 0.88 and CR = 2.8; for IL-8, Ri = 0.66 and CR = 2.2; and for ECP, Ri = 0.82 and CR = 1.1. We conclude that the repeatability of cellular and soluble markers of inflammation in induced sputum from patients with mild and moderate-to-severe asthma is satisfactory. Hence, induced sputum, processed by using the whole expectorated sample, seems to be a valuable method to monitor airway inflammation in asthma.