抽象的
(D-ALA2,NMEPHE4,GLY-OL5)脑膜素(DAMPO),一种选择性u-Apioid受体激动剂先前已经证明抑制豚鼠气道中的胆碱能和非胆管能收缩。相比之下,当在8Hz刺激时,阿片类药物对上气管中的胆碱能神经递质没有抑制作用。我们研究了该抑制,一种选择性Mu-阿片受体激动剂[D-PEN 2,5]脑(DPDPE),一种选择性δ-阿片受体激动剂和U-69593,一种选择性的Kappa-ApioID受体激动剂可以调节胆碱能在不同频率的上气管中的收缩。此外,我们调查了该死的,DPDPE和U-69593还可以调节天文放松。该死的(1-100 microM)抑制了上气管中的胆碱能收缩,最大抑制为57 +/- 15%,在1 Hz(n = 4; p <0.05)。另一方面,DPDPE(10 microm)和U69593(10 microM)没有产生胆碱能收缩的任何显着抑制。纳洛酮,一种阿片类受体拮抗剂(100 microM),能够拮抗该损伤在2Hz的胆碱能收缩上的抑制作用(n = 5; p <0.01)。该变量没有使累积浓度反应关系(10nm-10mm)移位(n = 4; ns)。这提供了证据表明,前常阿片受体(而不是δ-阿片类药物或Kappa-ApioID受体)调节上气管中的胆碱能收缩。 In contrast, DAMGO (10 microM) had no significant inhibitory effect on the nonadrenergic relaxation (n=4; NS) in the upper trachea. Neither DPDPE nor U-69593 had any effect on the nonadrenergic relaxation. These findings suggest that DAMGO directly inhibits the cholinergic contraction and that the opioid receptor involved in the inhibition of the cholinergic contraction in the upper trachea is of the mu-opioid type. The finding that opioids inhibit cholinergic contraction without altering NANC relaxations suggests that distinct populations of nerves mediate these two effects.