摘要
黏液的粘附可能会影响呼吸道的细菌定植。临床从呼吸道分离的非分型流感嗜血杆菌(NTHi)常呈流苏状。我们想知道有流苏的菌株是否与相关的非流苏菌株有不同的粘附性。一种微量滴定板试验已开发用于研究非类型流感嗜血杆菌粘附黏液。用无菌粘液样分泌物的溶胶相或纯化的粘液蛋白制剂孵育包被孔。培养至对数中期,清洗后加入孔中,两对实验室用的流苏状(F+)和非流苏状(F-)非分型流感嗜血杆菌,以及6株新鲜的临床分离的流苏状非分型流感嗜血杆菌,每个菌株都有非流苏状的伴侣,它们都是通过琼脂上的连续传代获得的。然后在37摄氏度下孵育30分钟,然后清洗以去除未结合的细菌。用0.5% Tween 80搅拌解吸粘附菌,并进行活菌计数。两株流苏状实验室菌株(n = 12和n = 17)和6株流苏状临床菌株中的5株比它们各自的非流苏状伙伴更粘附于sol相。两个非流苏状的临床分离株比其流苏状的伙伴对塑料的粘附性更强。 A fimbriated laboratory strain was more adherent than its nonfimbriated partner both to a purified preparation of high molecular mass mucin and to the glycopeptide fraction of the same. We conclude that fimbriated strains of nontypable H. influenzae have increased adherence to sol phase of mucus and purified human respiratory tract mucin. The interactions of fimbriae with mucus are likely to be complex, and may involve both nonspecific and specific interactions.