抽象
铜绿假单胞菌感染常见的囊性纤维化患者和支气管扩张呼吸道。它产生几个毒素慢纤毛摆动,黏液刺激生产和破坏上皮。它坚持上皮细胞,受损的粘膜(在动物模型),和粘液。然而,鲜为人知的是铜绿假单胞菌与完整的人呼吸道黏膜的相互作用。我们研究了与空气粘膜相间绿脓杆菌的新型器官培养模型绿脓杆菌腺样组织的nonmucoid临床分离物的相互作用(5.9 +/- 0.9×10(6)菌落形成单位(cfu))在加湿气氛中移液至器官培养表面,并且温育15分钟,1,2,4,8,12,16,和24小时,在37摄氏度,在5%CO 2。评估已通过透射电镜和扫描电子显微镜制作。透射电子显微镜(TEM)表明,未感染的器官培养具有正常的超微结构。在8小时感染器官培养物的TEM显示显著上皮损伤:细胞从上皮表面挤压的43.9 +/- 10%,而纤毛的损失细胞的17.7 +/- 3%,与线粒体细胞的32.9 +/- 10.2%损坏,并与细胞质空泡化细胞的11.6 +/- 3%。铜绿假单胞菌仅偶尔粘附于正常上皮细胞,但粘附到上皮损伤的领域,并基底膜。 Scanning electron microscopy (SEM) of organ cultures up to 2 h found P. aeruginosa only infrequently associated with mucus. SEM at 4 h revealed P. aeruginosa predominantly associated with mucus and extruded damaged epithelial cells, but also occasionally associated with cilia, and very occasionally with unciliated cells.(ABSTRACT TRUNCATED AT 250 WORDS)