抽象的
甲苯二异氰酸酯(TDI)是西方国家职业哮喘最常见的原因。本研究的目的是研究遗传因素是否参与甲苯二异氰酸酯诱导的哮喘。我们研究了三组受试者中的人白细胞抗原(HLA)II类遗传标志物的频率:1)受TDI诱导的哮喘(n = 30)的受试者;2)没有TDI诱导的哮喘病史的暴露受试者(n = 12);3)未暴露于TDI的正常受试者(n = 126)。从三组收集静脉血样,通过聚合酶链式反应(PCR)法扩增DQA和DQB基因的多态性第二外显子。E.valuation of HLA class II gene products in TDI-induced asthma cases showed a positive association with HLA-DQB1 * 0503 and a negative association with HLA-DQB1 * 0501 alleles, which differed at residue 57 for a single amino acid, i.e. aspartic acid in DQB1 * 0503 and valine in DQB1 * 0501. No significant difference was found in the distribution of DQA1 alleles between asthmatics and controls. Our results confirm the hypothesis that HLA-DQB1 * 0503 has a role in conferring susceptibility to TDI-induced asthma and that residue 57 of HLA-DQB1 is a potentially critical location.