抽象的
在Al(OH)3中的皮下注射卵泡致敏的几内亚猪并提高2周后,将标记的支气管高反应性与各种激动剂和CD4 + T淋巴细胞和嗜酸性粒细胞强烈的支气管壁浸润。我们已经比较了FK506一种新型免疫抑制剂,对T细胞和嗜酸性粒细胞的粘膜浸润的效果与具有良好成熟的药物,Nedocromil钠和地塞米松的粘膜浸润。敏化的Hartley豚鼠用FK506(100微克kg-1每日)皮下治疗5天,Nedocromil钠(每日30微克kg-1)或地塞米松(每日200微克kg-1)。On the day of the experiment, i.e. one week after the booster injection of antigen, the animals were killed, the lungs dissected, frozen and cryostat sections stained by immunohistochemical methods using monoclonal antibodies specific for total T-lymphocytes, CD4+ and CD8+ T-cells. Cyanide-resistant eosinophil peroxidase activity was used to stain the eosinophils. Sections were coded and positive cells enumerated in the lamina propria and adventitia of the bronchi. Sensitized and antigen-stimulated vehicle-treated guinea-pigs showed marked infiltration of the bronchial wall by CD4+ T-lymphocytes and eosinophils compared with sensitized, non-antigen stimulated animals. As compared to vehicle, FK506 or dexamethasone abolished the T-cell/eosinophil invasion in the bronchial wall, whereas nedocromil sodium was ineffective in protecting the lungs from T-lymphocyte or eosinophil infiltration. We conclude that both FK506 and dexamethasone are effective in curtailing bronchial inflammation in allergic guinea-pigs, whereas nedocromil sodium did not resolve the inflammation associated with T-lymphocytes or eosinophils.