抽象的
长期存活的肺移植受者受到灭错支气管炎的出现和不可逆的气道阻塞的限制,例如,支气管炎梗塞综合征(BOS)。本研究研究了炎症细胞和它们的活化标志物是否在Bosboalveolar灌洗(BAL)和跨血栓的活组织检查(TBB)中增加了炎症细胞和它们的活化标志物。还评估了BAL流体中的抗氧化剂水平。与13名移植受者进行比较来自六个单肺,两种双侧肺和五种心肺移植患者的BAL流体和TBB,与13名没有BOS的移植受体。使用髓氧化酶(MPO),嗜酸性粒细胞阳离子蛋白(ECP)和白细胞介素(IL)-8的BAL流体水平分别用作中性粒细胞和嗜酸性粒细胞的活化和吸引的标志物。进行与MPO和ECP(EG2)的单克隆抗体的TBB的免疫组织化学染色。在博士患者中发现了嗜中性粒细胞和MPO水平的显着增加。该研究结果与TBB中MPO的单克隆染色程度相关。BOS患者在ECP和IL-8的BAL水平显着增加。 BAL concentrations of the water-soluble antioxidants ascorbate, urate and glutathione were generally lower in BOS patients. The results indicate that neutrophil infiltration and activation, as well as oxidative stress, may play a role in the development and/or progression of bronchiolitis obliterans syndrome. Markers for neutrophil activation could have a potential role in monitoring disease activity in patients with this syndrome.