抽象的
树突状细胞是人白细胞抗原(HLA)-DR阳性辅助细胞,在细胞介导的免疫反应的发展中起关键作用。由于胸膜空间经常参与细胞介导的免疫反应,因此我们试图将树突状细胞与胸膜液分离。胸膜积液单核细胞(PEMC)是通过从19例恶性胸膜炎患者中回收的透明性胸腔积液的Ficoll离心得出的。双步依从性之后,回收了牢固遵守的单核细胞(FAMS)和松散粘附的单核细胞(LAM)。将后一种细胞在牛血浆白蛋白梯度上离心,以获得松散的低密度分数。用绵羊红细胞(SRBC)玫瑰色细胞(SRBC)玫瑰细胞以获得非贴遗迹的非屈服(NANR)细胞分数。丝裂霉素C处理的PEMC,NANR,FAMS和LAM在混合白细胞反应实验中用作刺激性细胞。通过纯化的正常同种异体血液T淋巴细胞作为辅助细胞功能的指数评估,通过纯化的正常同种异体血液T淋巴细胞掺入3H-胸腺苷。NANR,FAM和LAM细胞的表型使用单克隆抗体(MoAbs)的单一和双染色表征。附件单元格(AC)活性(每分钟计数(CPM)x 10(3)(+/- se); 2.5 x 10(4)lam(148 +/- 24)和nanr(148 +/- 24)和NANR(108.4+/-11.2) was greater than that observed for FAM (59.3+/-9.4) and for unfractioned PEMC (13.8+/-4.9). The FAM fraction was virtually entirely composed of CD68+ HLA-DR+ mononuclear phagocytes. NANR and LAM contained 51+/-12% and 65+/-6% HLA-DR+ cells, respectively, and most HLA-DR positive cells were negative for CD3, CD19, and CD68, markers for T-, B-lymphocytes and mononuclear phagocytes. Moreover, both NANR and LAM fractions contained significant numbers of cells bearing the RFD1 surface marker, expressed on dendritic cells. These results suggest that dendritic cells are present in exudative pleural effusions, and that they may be involved in the development of cell-mediated immune reactions in the pleural space.