摘要
的存在下,亚细胞分布,物种特异性和经典的蛋白的可能缺氧刺激诱导易位激酶C在颈动脉体进行了检查(cPKC)同工酶。颈动脉体从体内暴露于常氧或急性缺氧条件下的猫和鼠常氧解剖。对于同种型特异性的免疫组化单克隆抗血清PKCα的,PKCbetaI,PKCbetaII和PKCgamma使用。所述免疫反应由异硫氰酸荧光素(FITC)标记可视化。FITC /德克萨斯红双标记标本为cPKC同工酶使用/酪氨酸羟化酶证明化学感受器细胞定位的cPKC同工酶。使用激光扫描共聚焦图像技术检测免疫荧光。结果表明,PKCα的和PKCgamma的表达,但不PKCbeta在颈动脉体化学感受器细胞的细胞质同种型。为cPKC的化学感受器细胞定位提供了证据,双标记同种型检测。免疫染色是最激烈的胞体,核膜,偶尔,核质的外围。没有大的差异,在PKCα的和PKCgamma的免疫定位常氧和缺氧条件下或物种之间发现的。 However, the immunoreactivity tended to accumulate more in the peripheral cytoplasm and away from the nucleus in the hypoxic chemoreceptor cell. This study demonstrates the presence of classical protein kinase C enzymes in chemoreceptor cells. The intensity of the immunoreactivity may suggest a role for the classical protein kinase C signalling pathway in shaping the hypoxic response at the carotid body. However, this study failed to provide firm evidence of this.