文摘
本研究的目的是:评估痰诱导方法的安全使用在儿童急性哮喘吸入生理盐水;并调查之间哮喘急性加重痰细胞计数的变化和它的分辨率。超声喷雾使用生理盐水诱导痰从儿童急性哮喘(n = 8)到1 h内再一次至少14天后,决议后恶化。儿童接受了预处理与支气管扩张剂和最大呼气流量(PEF)监控整个过程。样本分析细胞总数、微分细胞计数,并使用特定的免疫化学染色嗜酸性粒细胞、中性粒细胞。痰诱导了每个孩子急性哮喘没有不利影响。均值下降PEF从基线在痰诱导5.3%和3.4%急性发作的决议。雾化更短时间被要求导致急性哮喘痰比在随访(7.8 vs 13.9分钟;p = 0.04)。在急性哮喘,有一个强烈的细胞渗透(平均细胞总数34 x 10 (6) cells.mL-1),复苏后解决(1.9 x 10 (6) cells.mL-1) (p = 0.04)。 The infiltrate was heterogenous, comprising eosinophils (6.7 x 10(6) cells.mL-1), neutrophils (5.4 x 10(6) cells.mL-1) and mast cells (0.47 x 10(6) cells.mL-1). Resolution of the exacerbation was accompanied by a significant fall in eosinophils and neutrophils (p < or = 0.04). Normal saline induction of sputum can be used to assess airway inflammation in acute asthma. Children with acute asthma have intense airway inflammation that is heterogeneous and involves neutrophils, eosinophils and mast cells.