抽象
在人鼻气通气道中存在一氧化氮(NO),主要来自Paranasal鼻窦。原位杂交的免疫组织化学研究和信使核糖核酸(mRNA)表明,2型没有合成酶(NOS)在健康的鼻窦上皮中形成。通过研究L-精氨酸对来自窦粘膜的活组织检查的L-精氨酸对L-瓜氨酸的酶促转化,我们进一步表征了鼻窦活性。在重建面部手术期间从九个健康受试者获得上颌窦活检。此外,与用高系统剂量的糖皮质激素治疗的5名患者中发现的那些,鼻腔没有浓度。最后,I.V的效果。L-精氨酸输注在鼻腔上没有浓度在六个健康受试者中进行。在所有活组织检查中发现了CA(2 +) - 独立的NOS活性,分别比CA(2 +) - 依赖性活动高五倍(分别为179 +/- 64和36 +/17 pmol.g-1.min)。对照之间的鼻腔没有差异(344 +/- 21份每十亿(PPB))和类固醇治疗患者(342 +/- 36 ppb)。纳尔在i.v后没有增加高达35%。 infusion of L-arginine. We conclude that NOS activity in healthy sinus mucosa is predominantly Ca(2+)-independent and this NOS is not downregulated by systemic steroids. Furthermore, L-arginine infusion increases nasal airway NO excretion in vivo, indicating that the substrate concentration is a rate-limiting factor under basal conditions. These findings further support the notion that sinus NOS is identical or very closely related to the type-2 NOS; however, the regulation of expression seems to be fundamentally different from that described previously for this NOS isoform.