摘要
全身皮质类固醇治疗可影响膈肌结构和功能。我们假设,与每日口服甲强的松龙(MP)相比,隔天口服甲强的松龙(MP)对大鼠膈肌的功能、免疫组化和生化特征的影响较小,而反复使用MP则更大。60只成年大鼠随机分为四组:生理盐水组;MP连续(MP- c), 1 mg。MP日交替治疗(MP- a), 2 mg。每隔一天Kg-1;MP- b, mp2mg。每日1公斤,连续2周,生理盐水4周,mp2mg。每天1公斤,持续2周。总疗程为8周。测量了隔离膜片的收缩性能。采用I、IIa、IIx、IIb型肌球蛋白重链反应性抗体进行免疫组化分析。 Biochemical evaluation included markers of fast energy supply, glycogenolytic activity, beta-oxidation capacity and oxidative capacity. The force-frequency curve was depressed in all MP groups. Fibre type I, IIx and IIb cross-sectional area (CSA) decreased in all MP groups. Burst therapy decreased the contribution of type IIb fibres to total diaphragm muscle area. MP-A affected glycogenolytic activity less than MP-C. Burst MP therapy reduced creatine kinase (CK) activity and beta-oxidation capacity compared to MP-C. Oxidative capacity was increased in all MP groups. In conclusion, although the methylprednisolone treatment regimens affected diaphragm muscle morphology and bioenergetic enzyme activities in different ways, force generation decreased in all methylprednisolone treated groups to the same extent.