抽象
在炎症反应的差异和隐原性纤维化肺泡炎(CFA)的预后以及与全身性硬化症相关联(FASSc)已开始出现。据推测,这些差异可能在T辅助(TH)-1和Th-2型细胞因子的不同的图案被反射。从CFA和FASSc的临床证据充分的情况下都获得开放肺活检和,作为对照,与来自吸烟者肺中,其(每组N = 5)被切除癌症获得大体和组织学正常薄壁组织进行比较。原位杂交(ISH)是适用于使用反义和感测样品35 S标记的核糖核酸探针来检测白介素信使核糖核酸(mRNA)的(IL)-2,IL-4,IL-5和干扰素(IFN) -伽马。表达所研究的细胞因子的细胞之间52-91%存在于肺泡间质,而不是在腔内的细胞或肺泡上皮衬里。Ťhe highest values for all four cytokines were present in the patients with FASSc, i.e., 22-39 ISH positive cells x mm(-2) alveolar tissue compared with 1-19 cells x mm(-2) and 4-5 cell x mm(-2) in CFA and control subjects, respectively. Whereas the proportions of the four cytokines in FASSc were similar to the control subjects, IL-4 and IL-5 predominated significantly in CFA (p<0.001). For example, the ratio of IL-5 to IFN-gamma was 22:1 in CFA, significantly higher than in the cases with FASSc (2:1) or the control subjects (4:1) (p<0.05). In conclusion, cryptogenic fibrosing alveolitis is an inflammatory condition which is characterized, like asthma, by a predominance of gene expression for T-helper-2-type regulatory cytokines, whereas cryptogenic fibrosing alveolitis associated with systemic sclerosis appears to have a distinct mixed T-helper-1/T-helper-2 functional phenotype and a greater number of cells expressing each of these pro-inflammatory cytokines.