@article {Chanez1002作者= {Chanez, P和削弱,G和汤川,T和巴恩斯PJ涌,KF}, title ={代氧自由基从血液中嗜酸性粒细胞哮喘患者刺激后拥堵或佛波醇酯},体积={3}={9},页面={1002 - 1007}={1990},出版商={欧洲呼吸学会},188bet官网地址嗜酸性粒细胞(EOS)可能在支气管哮喘的病理生理中发挥重要作用，因为它们能释放对支气管上皮细胞有细胞毒性的氧自由基和几种碱性蛋白。我们研究了从有哮喘症状(AS, n = 7)或鼻炎症状(AR, n = 4)或无症状(AA, n = 5)的特应性受试者(AA, n = 5)和高嗜酸性粒细胞综合征(HES, n = 5)受试者的血液中分离的EOS的反应。采用甲酰胺密度梯度分离EOS，采用血小板活化因子(PAF, 100 nM)或佛波12-肉豆杉-13-乙酸酯(PMA, 100 nM)体外活化。氧自由基的生成通过荧光素增强化学发光(CL)测定。EOS纯度为83 +/- 1.7\%(平均值+/- SEM)，存活率大于95\%。HES对EOS的CL反应显著高于AA (p < 0.01)和AR (p < 0.05)。与AR (17 +/- 13 mV, p < 0.01)和AA (23 +/- 14 mV, p < 0.01)相比，AS (PAF诱导的CL = 90 +/- 27 mV)的态密度EOS对PAF的反应更明显。PMA也得到了类似的结果。从HES受试者得到的低密度EOS与从as得到的正常密度EOS对PMA和PFA的反应相同。 Thus, EOS from AS have an enhanced potential for activation, particularly by PAF; this may represent an important mechanism for the perpetuation of the inflammatory response in asthma, since EOS can also release PAF.}, issn = {0903-1936}, URL = {//www.qdcxjkg.com/content/3/9/1002}, eprint = {//www.qdcxjkg.com/content/3/9/1002.full.pdf}, journal = {European Respiratory Journal} }