TY -的T1 -验证炎症介质的化验痰JF -欧洲呼吸杂志》乔欧元和J SP - 778 LP - 781 - 10.1034 / j.1399-3003.2000.15d24。x六世- 15 - 4非盟- RA Stockley盟- DL贝利Y1 - 2000/04/01 UR - //www.qdcxjkg.com/content/15/4/778.abstract N2 -商用免疫分析研究中越来越频繁的使用肺部炎症。然而,他们的表现在复杂生物液体很少评估。作者希望评估其可靠性,以确定结果的痰样本很容易解释。几个这样的化验的再现性因此决定一起恢复已知数量的纯试剂的能力。痰液溶胶阶段是获得一些慢性肺部疾病患者和一起使用的试剂在一系列的“飙升”和dilutional实验。结果确认髓过氧物酶和免疫酶测定interleukin-8化验,白三烯B4和分泌leukoproteinase抑制剂都重现(intra-assay变异系数3.8 -7.7%)。Furthermore, each of these assays gave >85% recovery of a "spike" with pure reagent. However, the immune assay for myeloperoxidase (although reproducible) gave poor recovery and was dependent on the degree of sample dilution and elastase content. These studies confirm that the reliabilities of fluid phase measurements should be assessed before being widely applied to complex biological samples. ER -