TY - JOUR T1 -各种脂多糖释放肺上皮细胞和成纤维细胞单核细胞趋化活性的潜力JF -欧洲呼吸杂志JO - Eur Respir J SP - 545 LP - 552 VL - 14 IS - 3 AU -小山，S AU -佐藤，E AU -野村，H AU -久保，K AU -三浦，M AU -山下，T AU -永井，S AU -泉，T Y1 - 99/09/01 UR - //www.qdcxjkg.com/content/14/3/545.abstract N2 -尽管来自铜绿假单胞菌的脂多糖(LPS)的细胞毒性，即limus amoebobocyte lysate活性，低于大肠杆菌0127:B8，铜绿假单胞菌诱导显著的持续肺部炎症，如囊性纤维化。本研究旨在检测从大肠杆菌和铜绿假单胞菌中获得的几种lps释放肺细胞单核细胞趋化活性(MCA)的潜力。lps差异刺激A549细胞、BEAS-2B细胞和肺成纤维细胞释放MCA (P. aeruginosa >055:B5 from Sigma >026:B6 (Sigma))。大肠杆菌0127:B8(西格玛)和0111:B4(西格玛)不刺激这些细胞。MCA采用棋盘分析法确定。分子筛柱层析发现了4个趋化峰。环己酰亚胺和脂氧合酶抑制剂抑制MCA的释放。阻断抗体实验表明，MCA的大部分是继发于单核细胞趋化蛋白-1 (MCP-1)和粒细胞-巨噬细胞集落刺激因子(GM-CSF)。 Thus, the concentrations of these chemoattractants were examined and it was found that the potency of the various LPSs to stimulate MCA closely paralleled their potency in releasing MCP-1 and GM-GSF. Serum augmented the release of MCP-1 and GM-CSF. However, the differences among LPSs from E. coli and P. aeruginosa in stimulating A549 cells were observed. These data suggest that Pseudomonas aeruginosa lipopolysaccharide may stimulate lung cells to release more monocyte chemotactic activity than lipopolysaccharides derived from Escherichia coli, leading to sustained prominent lung inflammation. ER -