TY - T1的潜在的各种脂多糖释放单核细胞趋化现象的活动从肺上皮细胞和成纤维细胞JF -欧洲呼吸杂志》乔和J SP - 545 LP - 552欧元六世- 14 - 3盟小山,年代盟,佐藤E盟——野村证券(Nomura) H AU - Kubo说,K盟——三浦,M盟,山下式T非盟- Nagai年代非盟和泉,T Y1 - 1999/09/01 UR - //www.qdcxjkg.com/content/14/3/545.abstract N2 -尽管细胞毒性的脂多糖(LPS)来自铜绿假单胞菌,即鲎变形细胞溶解产物活动,不如从强有力的大肠杆菌0127:B8,铜绿假单胞菌诱发突出持续肺部炎症,如囊性纤维化。本研究旨在考察潜在的多个有限合伙人来自大肠杆菌和铜绿假单胞菌释放活动(MCA)从肺细胞单核细胞的趋化作用。LPSs differentially stimulated A549 cells, BEAS-2B cells and lung fibroblasts to release MCA (P. aeruginosa >E. coli 0127:B8 from Difco >055:B5 from Sigma >026:B6 (Sigma)). E. coli 0127:B8 (Sigma) and 0111:B4 (Sigma) did not stimulate these cells. MCA was determined by means of checkerboard analysis. Molecular sieve column chromatography revealed four chemotactic peaks. The release of MCA was inhibited by cycloheximide and lipoxygenase inhibitors. Experiments with blocking antibodies suggested that much of the MCA was secondary to monocyte chemoattractant protein-1 (MCP-1) and granulocyte-macrophage colony-stimulating factor (GM-CSF). Thus, the concentrations of these chemoattractants were examined and it was found that the potency of the various LPSs to stimulate MCA closely paralleled their potency in releasing MCP-1 and GM-GSF. Serum augmented the release of MCP-1 and GM-CSF. However, the differences among LPSs from E. coli and P. aeruginosa in stimulating A549 cells were observed. These data suggest that Pseudomonas aeruginosa lipopolysaccharide may stimulate lung cells to release more monocyte chemotactic activity than lipopolysaccharides derived from Escherichia coli, leading to sustained prominent lung inflammation. ER -