% 0期刊文章%莫龙,卡梅拉% Smirnova,纳塔莉亚f % Jeridi,艾莎% Kneidinger,尼古拉斯% Hollauer,克里斯汀%思楚普,乔纳斯基督教%卡明斯基,Naftali来说% Jenne, Dieter % Eickelberg, Oliver % Yildirim阿里出席% T组织蛋白酶B促进胶原蛋白合成,使闭塞性细支气管炎综合征2021% % D J R 10.1183/13993003.01416 -2020%欧洲呼吸杂志% P 2001416 % V 57% N 5% X闭塞性细支气管炎综合征(BOS)是肺移植术后主要并发症(第)。BOS的特点是大量的支气管旁纤维化,导致空气trapping-induced肺功能障碍。溶酶体组织蛋白酶B,半胱氨酸蛋白酶,可以加强纤维通路在一些疾病。然而,组织蛋白酶B在BOS进展的相关性尚未解决。这项研究的目的是阐明组织蛋白酶B在BOS发病机制的功能。我们决定组织蛋白酶B水平支气管肺泡灌洗液(BALF)和肺组织健康的捐赠者(HD)和BOS肝移植患者。组织蛋白酶B活动评估通过荧光共振能量transfer-based测定和蛋白表达决心利用免疫印迹,ELISA和免疫染色。探讨组织蛋白酶B在BOS的病理生理学的影响,我们使用了一个体内原位肝移植小鼠模型。机械的研究进行体外使用巨噬细胞和成纤维细胞细胞系。我们发现显著增加组织蛋白酶B BOS患者BALF和肺组织的活动,以及在我们的小鼠淋巴细胞性细支气管炎模型。 Moreover, cathepsin B activity was associated with increased biosynthesis of collagen and had a negative effect on lung function. We observed that cathepsin B was mainly expressed in macrophages that infiltrated areas characterised by a massive accumulation of collagen deposition. Mechanistically, macrophage-derived cathepsin B contributed to transforming growth factor-β1-dependent activation of fibroblasts, and its inhibition reversed the phenotype.Infiltrating macrophages release active cathepsin B, thereby promoting fibroblast activation and subsequent collagen deposition, which drive BOS. Cathepsin B represents a promising therapeutic target to prevent the progression of BOS.CatB is a new biomarker and therapeutic target for early bronchiolitis obliterans syndrome after lung transplantation https://bit.ly/36vOXHc %U //www.qdcxjkg.com/content/erj/57/5/2001416.full.pdf