RT期刊文章SR电子T1 Epigenome-wide协会研究DNA甲基化和成人哮喘在农业肺部健康研究摩根富林明欧洲呼吸杂志乔和J FD欧元欧洲呼吸学会SP 2000217 10.1183/13993003.00217 -2020签证官56是3 A1 Thanh t黄平君A1 Sinjini Sikdar A1陈健徐A1 Mi Kyeong李A1乔纳森Cardwell A1埃里克《A1188bet官网地址美狄亚Imboden A1小桢A1 anne - marie Madore A1康康舞齐王A1天元A1布莱恩·d·贝内特A1詹姆斯·m·沃德A1 Christine A1劳拉e . g .公园Beane-Freeman A1 Debra王A1艾莉森Motsinger-Reif A1 David m . Umbach A1 Annah b Wyss A1 David a . Schwartz A1胡安·c·拉普莱斯青瓷A1凯瑟琳A1卡罗尔欧博A1妮可Probst-Hensch A1伊凡娜杨诉A1杰拉德h . Koppelman A1斯蒂芬妮·J·伦敦年2020 UL //www.qdcxjkg.com/content/56/3/2000217.abstract AB Epigenome-wide甲基化的研究支持儿童哮喘的表观遗传机制的作用;然而,研究成人很少见,很少有研究non-atopic哮喘。我们进行了最大epigenome-wide协会研究(ewa)的血液DNA甲基化在成人关系non-atopic和过敏性哮喘。我们测量血液中DNA甲基化使用Illumina公司MethylationEPIC数组中当前病例对照研究的2286名参与者成人哮喘嵌套在美国农业队列。异位性被定义为特定血清免疫球蛋白E (IgE)。参与者被归类为特异反应性没有哮喘(n = 185), non-atopic哮喘(n = 673)、过敏性哮喘(n = 271),或者一个参考群特异反应性和哮喘(n = 1157)。使用逻辑回归进行了分析。没有观察到协会有特异反应性没有哮喘。众多cytosine-phosphate-guanine (CpG)网站不同甲基化在non-atopic哮喘(8 family-wise错误率(fw) p < 9×10−8, 524错误发现率(罗斯福)小于0.05),与382年的小说《基因。更多CpG网站被确定在过敏性哮喘(181年弗兰克-威廉姆斯,1086年罗斯福),与569年的小说《基因。 104 FDR CpG sites overlapped. 35% of CpG sites in non-atopic asthma and 91% in atopic asthma replicated in studies of whole blood, eosinophils, airway epithelium, or nasal epithelium. Implicated genes were enriched in pathways related to the nervous system or inflammation.We identified numerous, distinct differentially methylated CpG sites in non-atopic and atopic asthma. Many CpG sites from blood replicated in asthma-relevant tissues. These circulating biomarkers reflect risk and sequelae of disease, as well as implicate novel genes associated with non-atopic and atopic asthma.Distinct methylation signals are found in non-atopic and atopic asthma. Most are related to gene expression and are replicated in asthma-relevant tissues, confirming the value of blood DNA methylation for identifying novel genes linked in asthma pathogenesis. https://bit.ly/2VnbJg3