@article {Hoang2000217作者= {Thanh t黄平君和Sinjini Sikdar陈健徐和Mi Kyeong李和乔纳森·Cardwell和埃里克《美狄亚Imboden和小桢anne - marie Madore康康舞气和天元小王和布莱恩·d·班尼特和詹姆斯·m·沃德和克里斯汀·g .公园和劳拉e . Beane-Freeman Debra国王和艾莉森Motsinger-Reif David m . Umbach Annah b . Wyss和大卫·a·施瓦茨和胡安·c·肿物{\ ' o} n和凯瑟琳拉普莱斯和卡罗尔欧博和妮可Probst-Hensch伊凡娜诉杨和杰拉德·h·Koppelman斯蒂芬妮·j·伦敦},title = {Epigenome-wide协会研究DNA甲基化和成人哮喘在农业肺部健康研究},体积= {56}= {3},elocation-id = {2000217} = {2020}, doi ={10.1183/13993003.00217 -2020},出版商={欧洲呼吸学会},文摘= {Epigenome-wide甲基化的研究支持儿童哮喘的表观遗传机制的作用;188bet官网地址然而,研究成人很少见,很少有研究non-atopic哮喘。我们进行了最大epigenome-wide协会研究(ewa)的血液DNA甲基化在成人关系non-atopic和过敏性哮喘。我们测量血液中DNA甲基化使用Illumina公司MethylationEPIC数组中当前病例对照研究的2286名参与者成人哮喘嵌套在美国农业队列。异位性被定义为特定血清免疫球蛋白E (IgE)。参与者被归类为特异反应性没有哮喘(n = 185), non-atopic哮喘(n = 673)、过敏性哮喘(n = 271),或者一个参考群特异反应性和哮喘(n = 1157)。使用逻辑回归进行了分析。没有观察到协会有特异反应性没有哮喘。大量磷酸胞嘧啶{\ textendash} {\ textendash}鸟嘌呤(CpG)网站不同甲基化在non-atopic哮喘(8 family-wise错误率(fw) p \ < 9 {\ texttimes}可达,524错误发现率(罗斯福)小于0.05),与382年的小说《基因。更多CpG网站被确定在过敏性哮喘(181年弗兰克-威廉姆斯,1086年罗斯福),与569年的小说《基因。 104 FDR CpG sites overlapped. 35\% of CpG sites in non-atopic asthma and 91\% in atopic asthma replicated in studies of whole blood, eosinophils, airway epithelium, or nasal epithelium. Implicated genes were enriched in pathways related to the nervous system or inflammation.We identified numerous, distinct differentially methylated CpG sites in non-atopic and atopic asthma. Many CpG sites from blood replicated in asthma-relevant tissues. These circulating biomarkers reflect risk and sequelae of disease, as well as implicate novel genes associated with non-atopic and atopic asthma.Distinct methylation signals are found in non-atopic and atopic asthma. Most are related to gene expression and are replicated in asthma-relevant tissues, confirming the value of blood DNA methylation for identifying novel genes linked in asthma pathogenesis. https://bit.ly/2VnbJg3}, issn = {0903-1936}, URL = {//www.qdcxjkg.com/content/56/3/2000217}, eprint = {//www.qdcxjkg.com/content/56/3/2000217.full.pdf}, journal = {European Respiratory Journal} }