RT期刊文章SR电子T1肺动脉内皮细胞培养肺动脉导管气球的培养物：用于肺血管疾病的考虑因素188bet官网地址A1 Ventetuolo, Corey E. A1 Aliotta, Jason M. A1 Braza, Julie A1 Chichger, Havovi A1 Dooner, Mark A1 McGuirl, Donald A1 Mullin, Christopher J. A1 Newton, Julie A1 Pereira, Mandy A1 Princiotto, Amy A1 Quesenberry, PeterJ. A1 Walsh，Thomas A1 Whittenhall，Mary A1 Klinger，James R. A1 Harrington，Elizabeth O. YR 2020 UL //www.qdcxjkg.com/content.com/content/55/3/3/3/1901313肺动脉高压（PAH），但没有建立的方法来研究活着的患者肺动脉内皮细胞（PAEC）。我们试图从右心脏导管（RHC）中使用的肺动脉导管（PAC）气球（RHC）来培养培训，以表征成功的培养尝试并描述PAEC行为。在原发性培养物中生长至融合和内皮细胞表型。在三到八段之间进行了凋亡，迁移和管形成的标准测定。我们从45名受试者中收集了45个PAC提示，并从19种气球中获得了成功的PAEC培养物（39％）。在成功的尝试与失败尝试中，受试者人口统计细节或RHC程序细节没有差异。但是，对于符合PAH血液动力学标准的受试者，在成功尝试（19，53％中的10个中，有10个）与失败的尝试（30％，30％，30％）之间的尝试较高但不显着（p = 0.10）。成功的培养在患有较低心脏指数（P = 0.03）和较高肺血管耐药性（P = 0.04）的受试者中更有可能。与商业PAEC相比，具有特发性PAH受试者的PAEC具有抗凋亡性（P = 0.04），并且与具有高心输出量的portopulmonary高血压的PAEC相比，迁移降低（P = 0.01）。 PAECs from a subject with HIV-associated PAH formed fewer (p=0.01) and shorter (p=0.02) vessel networks compared to commercial PAECs.Sustained culture and characterisation of PAECs from RHC balloons is feasible, especially in PAH with high haemodynamic burden. This technique may provide insight into endothelial dysfunction during PAH pathogenesis.Pulmonary artery endothelial cells (PAECs) from pulmonary artery catheter balloons used during routine right heart catheterisation can be cultured and sustained to study endothelial cell dysfunction at various stages of pulmonary hypertension http://bit.ly/2RL6dTc