TY -的T1 CX3CR1-fractalkine轴驱动器动力学变化的单核细胞在纤维间质性肺疾病JF -欧洲呼吸杂志》乔和J - 10.1183/13993003.00460 -2019欧元六世- 55 - 2 SP - 1900460 AU Greiffo弗r . AU - Viteri-Alvarez也好盟——私生子,马里昂AU - Dietel,丹妮拉盟——Ortega-Gomez雅慕黛娜圣母AU -李,乔伊斯s . AU - Hilgendorff安妮AU - Behr Jurgen盟——Soehnlein奥利弗盟——Eickelberg奥利弗AU -费尔南德斯摘要N2 -循环免疫细胞群已被证实可导致肺间质性疾病(ILD)。在这项研究中，我们分析了循环和肺常驻单核细胞群，并评估了它们的表型和从血液到肺的招募。采用流式细胞术对105例受试者的血样进行定量分析，其中83例为单核细胞，83例为单核细胞(非特异性间质性肺炎，28例为单核细胞性肺炎，19例为与结缔组织疾病相关的单核细胞性肺炎)，22例为对照组。用免疫荧光和流式细胞术检测肺组织单核细胞的定位和丰度。单核细胞群的培养可以单独进行，也可以与内皮细胞一起进行，以评估碎形蛋白依赖的迁移模式。我们发现，与对照组相比，循环中的经典单核细胞(CM)在ILD中增加，而非经典单核细胞(NCM)则减少。CM丰度与肺功能呈负相关，NCM丰度与肺功能呈正相关。固体瘤患者血浆和肺中CCL2和CX3CL1浓度均升高。Fractalkine与纤毛支气管上皮细胞共定位，从而产生对肺的趋化梯度。 Fractalkine enhanced endothelial transmigration of NCM in ILD samples only. Immunofluorescence, as well as flow cytometry, showed an increased presence of NCM in fibrotic niches in ILD lungs. Moreover, NCM in the ILD lungs expressed increased CX3CR1, M2-like and phagocytic markers. Taken together, our data support that in ILD, fractalkine drives the migration of CX3CR1+ NCM to the lungs, thereby perpetuating the local fibrotic process.The compartmental imbalance of fractalkine mediates the migration of nonclassical monocytes into fibrotic lung tissues. Furthermore, nonclassical monocyte-derived cells show a M2-like and phagocytic phenotype in ILD lungs. http://bit.ly/2CMFWex ER -