@article {Greiffo1900460，作者= {Greiffo，Flavia的R.和比特里-Alvarez的，瓦莱里娅和Frankenberger，马里恩和Dietel，丹妮拉和特加-Gomez的，阿尔穆德纳和Lee，S.乔伊斯和Hilgendorff，Anne和洱，J {\”U】的RGen和Soehnlein，Oliver和Eickelberg，Oliver和费尔南德斯，伊希斯E.}，标题= {CX3CR1 {\ textendash} CXXXC趋轴线驱动纤维化间质性肺病的单核细胞的动力学变化}，体积= {55}，数= {2}，elocation-ID = {1900460}，年= {2020}，DOI = {10.1183 / 13993003.00460-2019}，出版商= {欧洲呼吸协会}，已经示出的188bet官网地址抽象= {循环免疫细胞群，以有助于间质性肺。疾病（ILD）在本研究中，我们分析了循环和肺驻地单核细胞群，并评估了其从血液中的表型和招募到在血液样本的ILD流式细胞分析的肺用于定量循环单核细胞在105名受试者中进行的：83与ILD（N = 36，N = 28和n = 19非特异性interstiTiAl金属肺炎，过敏性肺炎和结缔组织疾病相关的ILD，分别地），以及22所控制。单核细胞的定位和丰度是使用免疫荧光和肺组织的流式细胞术评估。单核细胞群中单独的或与内皮细胞，以评估CXXXC趋依赖性轮回图案培养。我们表明，循环的单核细胞古典（CM）在ILD分别增加与对照组相比，虽然非经典的单核细胞（NCM）明显降低。CM丰肺功能呈负相关，而NCM丰正相关。这两个CCL2和CX3CL1浓度血浆和ILD患者的肺部增加。 Fractalkine co-localised with ciliated bronchial epithelial cells, thereby creating a chemoattractant gradient towards the lung. Fractalkine enhanced endothelial transmigration of NCM in ILD samples only. Immunofluorescence, as well as flow cytometry, showed an increased presence of NCM in fibrotic niches in ILD lungs. Moreover, NCM in the ILD lungs expressed increased CX3CR1, M2-like and phagocytic markers. Taken together, our data support that in ILD, fractalkine drives the migration of CX3CR1+ NCM to the lungs, thereby perpetuating the local fibrotic process.The compartmental imbalance of fractalkine mediates the migration of nonclassical monocytes into fibrotic lung tissues. Furthermore, nonclassical monocyte-derived cells show a M2-like and phagocytic phenotype in ILD lungs. http://bit.ly/2CMFWex}, issn = {0903-1936}, URL = {//www.qdcxjkg.com/content/55/2/1900460}, eprint = {//www.qdcxjkg.com/content/55/2/1900460.full.pdf}, journal = {European Respiratory Journal} }