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391.011 634.677 0.51 -283.975 re
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BT
/F1 1 Tf
14.944 0 0 14.944 77.159 724.308 Tm
0.0209 Tc
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0.5311 -1.0319 TD
[(colony-stimulating)-332.6(factor)-330.2(sequester)-334(in)-336.4(lung)-332.6(microvessels)]TJ
8.4372 -1.0319 TD
[(but)-332.7(a)0.2(re)-333.7(slow)-332.3(to)-332.4(migrate)]TJ
/F2 1 Tf
10.959 0 0 10.959 142.866 656.107 Tm
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/F3 1 Tf
8.966 0 0 8.966 58.28 625.209 Tm
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T*
[(Sato,)-332(Y)84.1(.)-338(Kitagawa,)-329.2(J.C.)-338.1(Hogg.)]TJ
/F4 1 Tf
12.6016 0 TD
0 Tw
(#)Tj
/F3 1 Tf
0.999 0 TD
[(ERS)-334.2(Journals)-335.6(Ltd)-328.2(2000.)]TJ
/F5 1 Tf
-13.6006 -1.0559 TD
[(ABSTRACT)66.7(:)-278.7(Inflammatory)-283.2(mediators)-289.4(such)-280.6(as)-278.4(granulocyte)-285.1(colony-stimulating)-284.9(factor)]TJ
T*
[(\(G-CSF\))-253.1(release)-239.4(polymorphonuclear)-254.1(leukocytes)-255.2(\(PMNL\))-249.9(from)-239.5(the)-257(bone)-247.8(marrow)63(.)-249.5(This)]TJ
T*
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T*
[(behaviour)-283.9(of)-297.1(leukocytes)-299.4(at)-297.1(the)-295(inflammatory)-297(site)-299.5(is)-293.4(unclear)80.6(.)-293.8(This)-295.7(study)-294(examined)-299(the)]TJ
T*
[(sequestration)-248(and)-248.6(migration)-246.5(of)-246.5(PMNL)-249(r)13.4(eleased)-250.8(fr)12.9(om)-252.4(the)-244.4(bone)-247.8(marrow)-234.2(by)-244.2(G-CSF)-252.3(in)-246.6(a)]TJ
T*
[(model)-333.8(of)-335.1(streptoc)9.4(occal)-334.5(pneumonia.)]TJ
0.999 -1.0559 TD
[(Eight)-364.5(hours)-356(following)-365.8(the)-358.2(administration)-365.8(of)-360.4(either)-364.9(human)-354.1(G-CSF)-366.1(\(n)]TJ
/F6 1 Tf
29.5533 0 TD
(=)Tj
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[(6\))-360.4(or)-365.5(saline)]TJ
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(\(n)Tj
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[(3\))-341.4(in)-347.7(rabbits,)-342.2(a)-340.9(focal)]TJ
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[(Streptococcus)-344.5(pneumoniae)]TJ
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[(pneumonia)-340.1(was)-344.4(induced)-346.8(and)-343.4(the)]TJ
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/F8 1 Tf
25.7342 0 TD
(')Tj
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0.215 0 TD
(-bromo-2)Tj
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3.9455 0 TD
(')Tj
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(-deoxyuridine)Tj
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[(\(BrdU\))-416.2(was)-414(used)-413.4(to)-417.3(label)-411.6(PMNL)-413.4(\(PMNL)]TJ
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(BrdU)Tj
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0 Tc
[(\))-417.8(i)0(n)-411(the)-415.1(marrow)-404.9(and)-413(as)-411.2(a)-416.8(marker)-406.3(of)]TJ
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[(PMNL)-369.1(newly)-368.2(released)-363.9(by)-364.3(the)-370.9(bone)-368(marrow)63(.)-369.6(The)-368.3(PMNL)]TJ
6.725 0 0 5.977 276.945 506.154 Tm
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(BrdU)Tj
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0 Tc
[(in)-373(the)-370.9(lung)-364.2(and)-368.7(blood)]TJ
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[(wer)11.3(e)-334.4(identified)-340.7(using)-333.3(immunohistochemistry)48(.)]TJ
0.999 -1.0559 TD
[(G-CSF)-366.1(pr)15.3(etreatment)-356.3(elevated)-363.9(the)-364.5(cir)12.2(culating)-365.3(PMNL)-362.8(\(3.6\2130.4)-361.1(\(mean\213)]TJ
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(SEM)Tj
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[(\))-360.9(t)0(o)-360.4(8.3\213)]TJ
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(1)Tj
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(3)Tj
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(10)Tj
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(9)Tj
/F10 1 Tf
8.966 0 0 8.966 82.091 475.767 Tm
(.)Tj
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(L)Tj
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(-1)Tj
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0.2874 Tc
(,p)Tj
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1.0875 0 TD
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[(0.05\))-286.4(and)-280.2(PMNL)]TJ
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(BrdU)Tj
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[(\(5.4\2132.1)-285.2(to)-284.5(12.5\2133.1%,)-286.1(p)]TJ
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10.3822 0 TD
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[(0.05\))-286.4(counts)-286.9(at)-284.5(8)-284(h)-282.6(with)]TJ
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T*
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T*
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T*
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T*
[(circulating)-346.7(blood)-357.4(\(p)]TJ
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8.5043 0 TD
(<)Tj
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[(0.05\).)-352.5(In)-359.2(the)-358.2(G-CSF)-353.4(group,)-345(only)-359.3(1)51.1(1.2\2132.6%)-353.3(of)-354(the)-358.2(PMNL)-356.5(that)]TJ
-9.0544 -1.0559 TD
[(migrated)-263.4(into)-265.5(the)-263.4(airspaces)-263.6(wer)11.3(e)-258.5(PMNL)]TJ
6.725 0 0 5.977 210.331 420.945 Tm
0.0001 Tc
[(Br)-9.7(dU)]TJ
8.966 0 0 8.966 228.699 416.976 Tm
0 Tc
[(compar)10.8(ed)-262.9(to)-259.2(50.8\2138%)-261.9(PMNL)]TJ
6.725 0 0 5.977 341.235 420.945 Tm
0.0001 Tc
(BrdU)Tj
8.966 0 0 8.966 359.603 416.976 Tm
0 Tc
[(in)-259.2(the)]TJ
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[(pulmonary)-430(capillaries.)]TJ
/F7 1 Tf
10.1293 0 TD
[(In)-428.7(vitro)]TJ
/F5 1 Tf
3.661 0 TD
[(studies)-429.1(showed)-434.6(PMNL)]TJ
6.725 0 0 5.977 270.085 411.477 Tm
0.0001 Tc
[(Br)-9.7(dU)]TJ
8.966 0 0 8.966 289.984 407.509 Tm
0 Tc
[(r)13.4(eleased)-434.2(fr)12.9(om)-429.4(the)-434.1(bone)]TJ
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[(marrow)-322.7(by)-332.7(G-CSF)-334.5(are)-326.8(less)-331.3(deformable)-336.6(than)-337.5(unlabelled)-337.4(cir)12.2(culating)-333.7(PMNL)-337.5(\(p)]TJ
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33.0941 0 TD
(<)Tj
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[(It)-456.4(is)-457.8(concluded)-459.3(that)-460(granulocyte)-455.8(colony-stimulating)-468.3(factor)-453.7(treatment)-451.9(causes)-459(the)]TJ
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T*
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T*
[(pneumonic)-333.6(site.)]TJ
/F3 1 Tf
T*
[(Eur)-331.9(Respir)-333.8(J)-334.4(2000;)-333.5(15:)-334.6(1079\2611086.)]TJ
/F11 1 Tf
7.97 0 0 7.97 401.499 626.173 Tm
[(University)-387.3(of)-394.3(British)-396.9(Columb)12.3(ia,)-401.6(Pulmon-)]TJ
0 -1.0599 TD
[(ary)-1045.6(Research)-1037.9(Laboratory)67.4(,)-1044.6(S)0(t)-1048.7(Paul)]TJ
/F8 1 Tf
16.2108 0 TD
(')Tj
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0.2134 0 TD
(s)Tj
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[(Hospital,)-639.3(V)106.3(ancouver)41(,)-639.1(British)-638.7(Columbia.)]TJ
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(Canada.)Tj
0 -2.1197 TD
[(Corresponde)11.8(nce:)-255.1(S.F)80.8(.)-255(van)-255.9(Eeden,)-252.3(Pulmon-)]TJ
0 -1.067 TD
[(ary)-298.7(Research)-291.1(Laboratory)67.4(,)-297.7(S)0(t)-301.8(Paul)]TJ
/F8 1 Tf
13.2162 0 TD
(')Tj
/F11 1 Tf
0.2205 0 TD
[(s)-297.9(Hospi-)]TJ
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[(tal,)-458.8(V)106.3(ancouver)41(,)-461.3(B.C.)-458.3(V6Z)-454.1(1Y6,)-457.5(Canada.)]TJ
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[(Fax:)-325.1(1)-332.2(6048068351)]TJ
0 -2.1197 TD
[(Keywords)11.5(:)-334.6(Bone)-329.6(marrow)-323.8(release)]TJ
0 -1.067 TD
(5)Tj
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(-bromo-2)Tj
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(')Tj
/F11 1 Tf
0.2205 0 TD
[(-deoxy)13.4(uridine)]TJ
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[(polymorphon)13.2(uclear)-328.3(leukocytes)]TJ
T*
(migration)Tj
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[(str)30(eptococca)13.5(l)-334.6(pneumoni)13(a)]TJ
/F11 1 Tf
0 -2.1197 TD
[(Received:)-327.9(June)-326.1(20)-330.1(1999)]TJ
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[(Accepted)-325.4(after)-331.6(revision)-329.8(March)-321.1(8)-332.2(2000)]TJ
0 -2.1268 TD
[(This)-353.5(work)-349.9(was)-359.1(supported)-346.6(by)-358.6(the)-350.7(Medical)]TJ
0 -1.0599 TD
[(Research)-483.1(Council)-484.6(of)-493.9(Canada)-479.9(\(grant)-494.1(No.)]TJ
T*
[(4219\))-444.9(and)-447.9(the)-450.3(BC)-453.4(Lung)-449.5(Associatio)11(n.)-452.1(S.)]TJ
0 -1.067 TD
[(van)-405.3(Eeden)-402.7(was)-401.8(supported)-396.4(by)-408.4(a)-402.3(S)0(t)-408.5(Paul)]TJ
/F8 1 Tf
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(')Tj
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(s)Tj
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[(Hospital)-490.9(Foundation)-492.7(Scholarshi)12(p)-503(and)-497.7(E.)]TJ
T*
[(Lawrence)-290.3(by)-301.7(a)-295.7(fellowship)-294.9(from)-298.1(the)-301(Medi-)]TJ
0 -1.067 TD
[(cal)-214.6(Research)-205.7(Council)-207.1(of)-209.4(Canada)-209.5(and)-213.2(Gene-)]TJ
0 -1.0599 TD
[(tech)-326.3(Inc.)]TJ
9.963 0 0 9.963 68.258 324.51 Tm
[(Po)16.3(ly)18.9(mo)18.1(rp)13.9(ho)21.2(nu)15.5(cl)18.4(ea)14.7(r)-463.3(l)0(e)18.4(u)0(k)15.5(o)0(c)20.8(y)0(t)13.2(e)0(s)-458.4(\()0(P)20.4(M)0(N)15.3(L)0(\))-456.8(a)0(r)13.5(e)-462.1(a)-467.8(c)0(r)13.5(i)0(t)22.2(i)0(c)12.8(a)0(l)]TJ
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[(co)15.1(mp)18.1(on)21.2(en)15.1(t)-395.8(o)0(f)-390.1(t)13.9(he)-388.9(bo)15.5(dy)]TJ
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(')Tj
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[(s)-394.6(d)0(e)15.1(f)0(e)19.2(n)0(c)15.1(e)-393.8(ag)15.1(ai)18.4(ns)20(t)-395.8(a)0(c)14.7(u)0(t)18.9(e)-399.5(ba)20.8(ct)18.4(er)13.5(ia)18.4(l)]TJ
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[(in)18.9(fe)13.5(ct)18.5(io)18.9(n)-308.1([)0(1)13.9(])0(.)-321.4(Mature)-324.3(PMN)-7.4(L)-323.6(respond)-321.5(to)-322.6(mic)-8.2(robial)-324.3(invas)-7.8(-)]TJ
T*
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T*
[(lular)-306.5(killing)-305.1(of)-299(the)-301(microbes)]TJ
/F3 1 Tf
11.5461 0 TD
0.0069 Tc
[(vi)6.9(a)]TJ
/F11 1 Tf
1.5137 0 TD
0 Tc
[(the)-301(release)-302.3(of)-304.7(hydrolyti)-9.9(c)]TJ
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[(enzym)-7.8(es)-401.5(and)-401(generat)-10.5(ion)-397.3(o)-6.5(f)-395(oxy)-8(gen)-401(free)-405.4(radicals)-407.5([1,)-401.8(2].)]TJ
T*
[(Since)-233.8(PMN)-7.4(L)-226.8(have)-231.5(a)-228.8(lim)-10.2(ited)-230.2(lifespan)-236.4(in)-231.5(the)-232.7(circu)-8.6(lation)-234.1(\(6\261)]TJ
T*
[(10)-280.4(h\),)-282.3(thei)-8.2(r)-281.2(suppl)-7.4(y)-279.6(must)-282.9(con)-8.4(stantly)-287.2(be)-280.8(reple)-8.6(nished)-287.4(by)-280.4(the)]TJ
0 -1.0698 TD
[(bone)-447.3(marr)-9.8(ow)-449.3([3].)-449.7(The)-445(bone)-453(marrow)-453.4(norm)-7.8(ally)-446.4(relea)-9(ses)]TJ
0 -1.0641 TD
(~1)Tj
/F9 1 Tf
1.0357 0 TD
(3)Tj
/F11 1 Tf
0.8024 0 TD
(10)Tj
7.472 0 0 6.641 86.57 233.461 Tm
-0.0296 Tc
(11)Tj
9.963 0 0 9.963 96.265 229.039 Tm
0 Tc
[(PMN)-7.4(L)-243.9(per)-249(day)-253.1([3\2615])-251.7(and)-247.4(the)-249.8(abil)-8.2(ity)-246.1(of)-253.5(the)-249.8(bone)]TJ
-3.8127 -1.0641 TD
[(marro)-10.6(w)-397.3(t)0(o)-402.2(increase)-405.5(this)-407.3(output)-402(durin)-7.8(g)-399.1(a)0(n)-400.3(infec)-8.6(tion)-400.5(is)-403.4(a)]TJ
T*
[(cruci)-8.6(al)-368.5(facto)-8.6(r)-372.2(i)0(n)-368.1(the)-375(hosts)-372.8(abil)-8.2(ity)-371.2(to)-373.8(control)-376.3(and)-372.6(survi)-9(ve)]TJ
T*
[(infec)-8.6(tions.)-436.9(An)-437.9(inabi)-9(lity)-437(to)-436.4(mount)-441.8(this)-435.8(host)-439.1(respon)-7.8(se)-435.6(to)]TJ
T*
[(infec)-8.6(tion)-417.5(is)-414.8(associ)-10.1(ated)-416(wi)-7.8(th)-413.6(a)-416.6(high)-420.8(morbidi)-9.1(ty)-413.6(and)-418.1(mor-)]TJ
T*
[(tality)-338.9(parti)-10.6(cularly)-336.9(in)-339.7(patients)-339.6(wi)-7.8(th)-334(neutrope)-10.9(nia)-335.1([6,)-333.6(7].)]TJ
1.0015 -1.0527 TD
[(Th)17.1(e)-359.7(u)0(s)14.4(e)-359.7(of)-355.9(th)18.9(e)-359.7(h)0(a)15.1(e)0(m)17.7(a)0(t)18.5(o)0(p)15.5(o)0(i)18.9(e)0(t)18.4(i)0(c)-357.1(g)0(r)19.6(o)0(w)17.3(t)0(h)-356.7(f)0(a)19.2(c)0(t)18.4(o)0(r)48.1(,)-358.9(gr)13.9(an)20.8(ul)13.2(o-)]TJ
-1.0015 -1.0641 TD
[(cy)15.1(te)-465.2(co)20.8(lo)13.2(ny)21.2(-s)18.4(ti)16.5(mu)12.4(l)13.9(a)0(t)12.8(i)0(n)18.9(g)-473.1(fa)13.5(ct)18.4(or)-464.1(\(G)15.7(-C)14.9(S)11.4(F)0(\))14.7(,)-472.7(to)-470.5(in)18.9(cr)19.2(ea)14.7(se)-464.1(th)13.2(e)]TJ
T*
[(nu)15.5(mb)18.1(er)-259.6(of)-264.9(ci)18.4(rc)19.2(ul)18.9(at)12.8(i)13.9(n)0(g)-263.3(P)11.4(MN)15.4(L)-272.3(a)0(n)15.1(d)-268.2(im)15.7(pr)19.6(ov)15.5(e)-268.6(t)0(h)18.9(e)-268.6(ou)15.5(tc)18.4(om)18.1(e)-274.3(i)0(n)]TJ
T*
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T*
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T*
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T*
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T*
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T*
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T*
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T*
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T*
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T*
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(m)Tj
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(g)Tj
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(.)Tj
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(kg)Tj
7.472 0 0 6.641 257.102 294.293 Tm
(-1)Tj
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[(s.)20.4(c.)]TJ
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(mL)Tj
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[(in)-242.9(py)15.5(ro)19.6(ge)15.1(n-)19.6(fr)18(ee)]TJ
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0 -1.0471 TD
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[(CSF)-403.8(\(12.5)]TJ
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(-1)Tj
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[(6\))-401.5(and)-401(rabbi)-8.2(ts)-403.4(in)-402.3(the)-403.4(contr)-8.2(ol)]TJ
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(3)Tj
/F11 1 Tf
0.8365 0 TD
(10)Tj
7.472 0 0 6.641 445.323 755.149 Tm
(8)Tj
9.963 0 0 9.963 451.899 750.727 Tm
(organisms)Tj
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(mL)Tj
7.472 0 0 6.641 508.875 755.149 Tm
(-1)Tj
9.963 0 0 9.963 515.055 750.727 Tm
[(\))-286.9(into)]TJ
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[(one)-446.6(lung)-449.3(and)-440.9(an)-445.8(equal)-450.9(volume)-448.7(of)-447(the)-443.2(vehicle)-449.5(into)-446(the)]TJ
0 -1.0528 TD
[(con)-8.4(tralateral)-238.2(lung.)-227.7(Blood)-232.8(samples)-236(\(3)-225.1(mL)-230.7(each\))-228.6(wer)-8.2(e)-223.1(taken)]TJ
0 -1.0471 TD
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0 -1.0471 TD
[(teri)-9.8(a.)]TJ
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[(S.)-206(pneu)-9.2(moniae)]TJ
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6.0434 0 TD
[(was)-212.7(instil)-9.6(led)-209.9(into)-212.7(the)-215.6(lungs)-212.2(foll)-9.4(owing)]TJ
-8.2513 -1.0527 TD
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T*
[(ana)-8.8(esthetize)-11.1(d)-325.1(with)-330.4(ketamine)-333.9(hydroch)-7.3(loride)-330(\(80\26110)-11.9(0)-325.1(m)0(g)]TJ
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22.5289 0.2049 TD
(.)Tj
/F11 1 Tf
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(kg)Tj
7.472 0 0 6.641 319.294 671.471 Tm
(-1)Tj
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(i.m.)Tj
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1.4909 0 TD
[(\))-275.5(a)0(n)-7.6(d)-273.9(xylazi)-10.1(ne,)-275.5(\(10\26115)]TJ
/F6 1 Tf
9.3097 0 TD
(m)Tj
/F11 1 Tf
0.5747 0 TD
(g)Tj
/F10 1 Tf
0.5008 0.1992 TD
(.)Tj
/F11 1 Tf
0.2731 -0.1992 TD
(kg)Tj
7.472 0 0 6.641 459.269 671.471 Tm
(-1)Tj
/F3 1 Tf
9.963 0 0 9.963 468.17 667.049 Tm
(i.m.)Tj
/F11 1 Tf
1.4909 0 TD
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[(S.)-274.3(p)-6.5(neumo-)]TJ
-18.2894 -1.0528 TD
(niae)Tj
/F11 1 Tf
2.2762 0 TD
(\(5)Tj
/F9 1 Tf
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(3)Tj
/F11 1 Tf
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(10)Tj
7.472 0 0 6.641 358.469 661.039 Tm
(8)Tj
9.963 0 0 9.963 367.824 656.56 Tm
[(organisms\))-559.7(was)-565.5(instil)-9.6(led)-562.8(into)-565.5(the)-562.8(lower)]TJ
-5.8726 -1.0471 TD
[(lobe)-432.6(of)-429.9(the)-431.9(anaest)-9.4(hetized)-432.4(rabbi)-8.2(t)-429.9(under)-432.6(fluoroscopy)52.7(.)-427.2(A)0(n)]TJ
0 -1.0527 TD
[(equ)-8.4(al)-465.2(volume)-465.8(of)-469.7(vehicle)-466.6(was)-468.8(instil)-9.6(led)-466(into)-463.1(the)-471.7(contra-)]TJ
0 -1.0471 TD
[(late)-8.6(ral)-234.3(low)-8.6(er)-231.2(lobe)-239.2(of)-236.4(the)-232.7(same)-241.5(rabbit)-239(as)-230.8(a)-234.5(contr)-8.2(ol.)-237.6(Animals)]TJ
0 -1.0527 TD
[(wer)-8.2(e)-473.5(kept)-478.1(sedat)-8.2(ed)-474.3(in)-476.2(a)-473.5(prone)-478.1(position)-481.1(with)-478.4(addit)-9(ional)]TJ
0 -1.0471 TD
[(ana)-8.8(esthetic)-396.9(as)-395.8(required)-399.4(throu)-7.8(ghout)-393.2(the)-392(ex)-7.6(periment)-10.7(.)-387.3(Fol-)]TJ
0 -1.0528 TD
[(low)-8.6(ing)-209.5(the)-215.6(last)-214.3(blood)-216.7(samp)-7.8(le,)-215.2(the)-209.9(anim)-9(als)-211.1(wer)-8.2(e)-211.7(killed)-217(with)]TJ
0 -1.0471 TD
[(an)-280.8(overdose)-277.1(o)-6.5(f)-269.8(sodiu)-7.4(m)-277.1(pentob)-7.4(arbitone,)-281.1(the)-278.2(chest)-281.3(was)-275.3(op-)]TJ
0 -1.0527 TD
[(ene)-8.8(d)-273.9(rapid)-8.2(ly)-277.1(and)-281.5(the)-278.2(base)-278.2(of)-282(the)-278.2(heart)-281.7(liga)-8.2(ted)-278.2(to)-277.1(mai)-8.2(ntain)]TJ
0 -1.0471 TD
[(the)-255.5(pulm)-8.6(onary)-250.5(b)-6.5(lood)-250.1(volum)-9.3(e.)-251.9(The)-251.5(trach)-8.6(ea)-252.7(and)-253.1(both)-250.1(lungs)]TJ
0 -1.0528 TD
[(wer)-8.2(e)-354(separa)-8.6(ted)-352.2(from)-362.2(other)-361(organs,)-349.6(weighed)-359.6(and)-355.5(inflated)]TJ
0 -1.0471 TD
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0 -1.0527 TD
[(buffer)-430.9(at)-436.8(25)-434(cmH)]TJ
7.472 0 0 6.641 382.45 529.398 Tm
(2)Tj
9.963 0 0 9.963 386.192 531.042 Tm
[(O)-437.2(press)-8.2(ure,)-437.1(reweighed)-442.8(and)-435.2(then)-438.3(im-)]TJ
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0 -1.0528 TD
[(wer)-8.2(e)-308.5(sliced)-312.9(horizontal)-313.1(to)-311.2(the)-306.7(plane)-314.3(of)-304.7(g)-6.5(ravity)-312.9(and)-304.3(blocks)]TJ
0 -1.0471 TD
[(of)-469.7(tissue)-473.1(were)-470.3(random)-9.7(ly)-464.8(selec)-8.6(ted)-466(from)-470.3(the)-466(pneu)-9.1(monic)]TJ
0 -1.0527 TD
[(veh)-8.4(icle-treated)-484.8(sites)-472.3(and)-475(untreated)-475.4(areas.)-480.5(These)-470.9(blocks)]TJ
0 -1.0471 TD
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0 -1.0528 TD
[(ing)-340.4(3-)]TJ
/F6 1 Tf
2.4356 0 TD
(m)Tj
/F11 1 Tf
0.569 0 TD
[(m-t)-9.4(hick)-335.9(sections.)]TJ
-2.0031 -1.0471 TD
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-1.0015 -1.0528 TD
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0 -1.0471 TD
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0 -1.0527 TD
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0 -1.0471 TD
[(we)16.9(re)-527.1(pe)15.1(rf)18(or)19.6(me)12(d)-530(u)0(s)14.4(i)13.9(ng)-525.1(a)-536.1(m)0(o)18.1(d)0(e)15.1(l)-532.4(S)11.4(S)0(8)16.3(0)-535.7(C)11.6(o)0(u)15.5(l)0(t)16.5(e)0(r)-527.1(C)11.6(ou)15.5(nt)18.9(er)]TJ
0 -1.0527 TD
[(\(C)14.9(ou)15.5(lt)16.5(er)-384.8(El)14.7(ec)20.4(tr)17.3(on)15.5(ic)18.4(s,)-389.3(Hi)14.9(al)18.4(ea)20.4(h,)-388.1(FL)17.9(,)-398.7(U)0(S)18.1(A)0(\))-394(a)0(n)20.8(d)-399.1(di)18.9(f)20.4(f)0(e)13.5(r)0(e)19.2(n)0(t)18.9(i)0(a)18.5(l)]TJ
0 -1.0471 TD
[(co)15.1(un)15.5(t)13.9(s)-286.5(we)16.9(re)-276.7(ma)17.7(de)-275.1(on)-274.7(W)37.9(r)0(i)17.3(g)0(h)15.5(t)]TJ
/F8 1 Tf
11.4039 0 TD
(')Tj
/F11 1 Tf
0.2049 0 TD
[(s-)12.8(st)17.7(ai)18.4(ne)20.8(d)-285.3(b)0(l)18.9(o)0(o)15.5(d)-285.3(sm)16.9(ea)20.4(rs)12.8(.)-279.2(B)0(l)14.1(o)0(-)]TJ
-11.6087 -1.0528 TD
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[(nu)15.5(mb)18.1(er)-236.8(of)-236.4(po)15.5(in)18.9(ts)-238.4(fa)19.2(ll)16.5(in)18.9(g)-251.2(o)0(n)-234.8(a)0(i)18.4(r)0(s)18.4(p)0(a)15.1(c)0(e)-235.7(a)0(n)15.1(d)-245.5(ti)16.5(ss)18.9(ue)-240.9(P)11.4(M)0(N)15.3(L)]TJ
7.472 0 0 6.641 269.235 455.301 Tm
0.0001 Tc
[(Br)24.5(dU)]TJ
9.963 0 0 9.963 58.28 440.39 Tm
0 Tc
[(an)15.1(d)-421.9(u)0(n)15.5(l)0(a)18.4(b)0(e)20.8(l)0(l)16.5(e)0(d)-417.3(P)11.4(MN)15.3(L)-426(i)0(n)-413.6(t)0(h)13.2(e)-422.3(ai)18.4(rs)18.4(pa)15.1(ce)-417.7(or)-412.8(in)-419.3(ti)16.5(ss)18.9(ue)-411.7(we)11.2(re)]TJ
T*
[(co)15.1(un)21.2(te)18.4(d)-438.9(a)0(n)20.8(d)-433.3(th)18.9(e)-439.3(V)12.4(v)-438.9(o)0(f)-424.2(e)0(a)20.4(c)0(h)-428.7(c)0(o)20.8(m)0(p)12.4(o)0(n)21.2(e)0(n)15.1(t)-435.6(w)12.4(a)0(s)-429.9(e)0(s)19.6(t)0(i)16.5(m)0(a)17.7(t)0(e)18.4(d)0(.)]TJ
0 -1.0528 TD
[(T)40.6(i)0(s)17.7(s)0(u)14.4(e)-376.7(i)13.9(n)-382(t)13.9(h)0(i)13.2(s)-371.8(co)15.1(nt)18.9(ex)15.1(t)-373(c)0(o)15.1(n)0(s)20(i)0(s)17.7(t)0(e)12.8(d)-376.3(la)18.4(r)20.4(g)0(e)15.1(l)0(y)-368.1(o)0(f)-367.3(a)0(l)18.5(v)0(e)15.1(o)0(l)18.9(a)0(r)-367.7(w)0(a)16.9(l)0(l)16.5(s)]TJ
0 -1.0471 TD
[(an)15.1(d)-279.6(i)0(n)18.9(c)0(l)18.4(u)0(d)15.5(e)0(d)-269.4(c)0(a)14.7(p)0(i)18.9(l)0(l)16.5(a)0(r)19.2(i)0(e)18.4(s)-280.8(an)15.1(d)-279.6(i)0(n)18.9(t)0(e)12.8(r)0(s)18.4(t)0(i)16.5(t)0(i)16.5(a)0(l)-271.8(s)0(p)20(a)0(c)14.7(e)0(s)19.6(.)-279.2(Re)16.1(su)20(lt)16.5(s)-280.8(a)0(r)13.5(e)]TJ
0 -1.0527 TD
[(ex)15.1(pr)19.6(es)19.6(se)13.9(d)-251.2(a)0(s)-242.1(t)0(h)13.2(e)-251.6(pe)20.8(rc)13.5(en)20.8(ta)18.4(ge)-246.6(of)-242.1(PM)14.4(NL)-242.9(in)-242.9(ai)12.8(rs)18.4(pa)20.8(ce)-247(or)-242.1(ti)16.5(ss)18.9(ue)]TJ
0 -1.0471 TD
[(th)18.9(at)-317.3(ar)19.2(e)-325.5(B)0(r)14.9(d)0(U)17.3(-)0(l)17.3(a)0(b)20.8(e)0(l)12.8(l)0(e)18.4(d)0(.)]TJ
1.0015 -1.0527 TD
[(Th)17.1(e)-319.8(n)0(u)21.2(m)0(b)12.4(e)0(r)-310.8(o)0(f)-310.4(P)11.4(MN)15.3(L)]TJ
7.472 0 0 6.641 158.003 382.053 Tm
0.0001 Tc
[(Br)24.5(dU)]TJ
9.963 0 0 9.963 177.392 377.631 Tm
0 Tc
[(or)-310.4(to)18.9(ta)18.4(l)-321.8(P)11.4(MN)15.3(L)-323.6(\()0(P)20.4(M)0(N)15.3(L)]TJ
7.472 0 0 6.641 269.631 382.053 Tm
[(to)26.4(ta)24.2(l)]TJ
9.963 0 0 9.963 282.217 377.631 Tm
(\))Tj
-22.4777 -1.0471 TD
[(in)-476.2(th)18.9(e)-490.6(a)0(i)18.5(r)0(s)18.4(p)0(a)15.1(c)0(e)-474.7(o)0(r)-481.1(t)0(i)16.5(s)0(s)18.9(u)0(e)-474.2(f)0(r)12.4(o)0(m)-477(t)0(h)18.9(e)-484.9(pn)15.5(eu)15.1(m)13.2(o)0(n)15.5(i)0(c)18.4(,)-489.8(ve)20.8(hi)18.9(cl)12.8(e-)]TJ
0 -1.0528 TD
[(tr)17.3(ea)14.7(te)18.4(d)-450.3(a)0(n)20.8(d)-456(un)21.2(tr)17.3(ea)14.7(te)18.4(d)-450.3(r)0(e)19.2(g)0(i)13.2(o)0(n)21.2(s)-451.5(we)16.9(re)-441.7(al)12.8(so)-440.9(ca)20.4(lc)12.8(ul)18.9(at)18.4(ed)15.1(.)-449.9(T)12.2(he)]TJ
T*
[(ca)14.7(l)13.9(c)0(u)15.1(l)0(a)18.4(t)0(i)16.5(o)0(n)-234.8(f)0(o)13.9(r)-247.1(th)18.9(e)-245.9(n)0(u)15.5(m)13.2(be)15.1(r)-247.1(o)0(f)-236.4(P)0(M)14.4(N)12.4(L)]TJ
7.472 0 0 6.641 200.863 350.702 Tm
0.0001 Tc
[(Br)24.5(dU)]TJ
9.963 0 0 9.963 219.515 346.224 Tm
0 Tc
[(in)-237.2(th)18.9(e)-245.9(a)0(i)12.8(r)0(s)18.4(p)0(a)20.8(c)0(e)-241.3(i)0(n)]TJ
-16.1839 -1.0471 TD
[(th)18.9(e)-325.5(p)0(n)15.5(e)0(u)20.8(m)0(o)18.1(n)0(i)13.2(c)-319.8(re)13.5(gi)18.9(on)-314.5(is)-318.1(sh)20(ow)17.3(n)-325.1(a)0(s)-316.1(a)0(n)-314.9(e)0(x)20.8(a)0(m)12(p)0(l)18.9(e)0(:)]TJ
ET
63.099 305.064 217.531 -0.454 re
f*
BT
/F12 1 Tf
9.963 0 0 9.963 63.099 320.258 Tm
[(Nu)14.3(mb)20(er)-327.5(of)-412.1(P)12.6(M)0(N)12.8(L)]TJ
6.974 0 0 6.974 144.454 323.887 Tm
0.0001 Tc
[(Br)33.1(dU)]TJ
9.963 0 0 9.963 161.461 320.258 Tm
0 Tc
[(in)-321.4(pn)12.2(eu)16.3(mo)20(ni)14.3(c)-331.2(a)0(i)13.2(r)0(s)18.9(p)0(a)16.7(c)0(e)]TJ
/F13 1 Tf
9.7479 0 TD
(\210)Tj
/F12 1 Tf
-19.621 -1.2007 TD
[(lu)14.3(ng)-324.7(vo)16.7(lu)14.3(me)]TJ
/F14 1 Tf
5.2353 0 TD
(|)Tj
/F12 1 Tf
1.3373 0 TD
[(V)12.4(v)-336.5(pn)17.9(eu)16.3(mo)14.4(n)11.8(i)0(c)-328.7(r)0(e)19.6(g)0(i)13.2(o)11.8(n)]TJ
/F14 1 Tf
9.1163 0 TD
(|)Tj
/F12 1 Tf
1.0015 0 TD
-0.0124 Tc
(Vv)Tj
/F13 1 Tf
6.974 0 0 6.974 241.398 311.924 Tm
0 Tc
(\205)Tj
/F12 1 Tf
0.3902 0 TD
-0.0158 Tc
[(PM)-15.9(N)6.8(L)]TJ
4.981 0 0 4.981 265.209 314.476 Tm
-0.0001 Tc
[(Br)39.3(dU)]TJ
/F13 1 Tf
6.974 0 0 6.974 277.398 311.924 Tm
0 Tc
(\206)Tj
/F12 1 Tf
9.963 0 0 9.963 157.946 295.54 Tm
[(14)21.2(3)-336.5(f)0(L)]TJ
/F11 1 Tf
-10.004 -1.2633 TD
[(wh)17.3(er)19.2(e)-365.4(1)0(4)15.5(3)-365(fL)-354.4(is)-357.9(th)18.9(e)-365.4(a)0(s)19.6(s)0(u)14.4(m)0(e)17.7(d)-365(vo)21.2(lu)13.2(me)-357.9(of)-355.9(a)-359.7(r)0(a)13.5(b)0(b)21.2(i)0(t)-359.1(P)11.4(MN)15.4(L)]TJ
0 -1.0471 TD
[([2)19.6(9].)-384.8(Results)-393.2(are)-391.7(expres)-8.2(sed)-390.8(as)-384.4(the)-392(tota)-8.2(l)-384.4(numbe)-7.4(r)-389.3(o)0(f)-390.1(PM-)]TJ
0 -1.0528 TD
(NL)Tj
7.472 0 0 6.641 71.49 266.457 Tm
(BrdU)Tj
/F10 1 Tf
9.963 0 0 9.963 87.987 264.019 Tm
(.)Tj
/F11 1 Tf
0.7398 -0.1992 TD
(mL)Tj
7.472 0 0 6.641 109.077 266.457 Tm
(-1)Tj
9.963 0 0 9.963 119.906 262.035 Tm
[(o)-6.5(f)-469(airspace)-479.5(or)-469.7(tissue)-478.8(in)-470.5(the)-471.7(pneum)-7.4(onic,)]TJ
-6.1856 -1.0528 TD
[(vehicl)-10.1(e-treated)-340.8(or)-338.9(untreated)-344.5(region.)]TJ
/F3 1 Tf
0 -3.4542 TD
[(Immuno)-8.3(fluo)-7.8(r)32.2(escence)-338.6(flow)-342.6(cytometr)-11.5(ic)-334.4(analys)-8.6(is)]TJ
/F11 1 Tf
1.0015 -2.0998 TD
[(Ex)17.1(pr)19.6(es)14(si)17.7(on)-246.2(of)-247.8(P)11.4(M)0(N)15.3(L)-261(su)20(rf)18(ac)14.7(e)]TJ
6.974 0 0 6.974 184.705 196.214 Tm
(L)Tj
9.963 0 0 9.963 188.844 196.214 Tm
[(-s)18.4(el)18.5(ec)14.7(ti)16.5(n)-251.2(a)0(n)15.1(d)-256.8(CD)18.3(18)-246.2(we)11.2(re)]TJ
-13.1053 -1.0527 TD
[(de)15.1(te)18.4(rm)16.5(in)18.8(ed)-417.3(by)-416.9(fl)17.3(ow)-415.2(cy)20.8(to)13.2(me)17.7(tr)17.3(y)-421.9(o)0(n)-416.9(w)0(h)17.3(o)0(l)18.9(e)-428(bl)18.9(oo)21.2(d)-427.6(s)0(a)19.6(m)0(p)12.4(l)0(e)18.4(s)]TJ
0 -1.0471 TD
[(co)15.1(ll)22.2(ec)14.7(te)18.4(d)-461.7(i)0(n)-447.8(E)0(D)18.9(T)80.5(A)-459.9(u)0(s)20(i)0(n)13.2(g)-456(me)17.7(th)18.9(od)15.5(s)-457.2(p)0(r)13.9(e)0(v)20.8(i)0(o)18.9(u)0(s)14.4(l)0(y)-447.8(d)0(e)15.1(s)0(c)19.6(r)0(i)17.3(b)0(e)15.1(d)]TJ
0 -1.0528 TD
[([1)19.6(9,)-342.6(21].)-357.1(A)-351.8(mous)-7.4(e)-354(monoclonal)-363.5(CD18)-353.1(\(DAK)-10.7(O\),)-354.6(DREG)-8.4(-)]TJ
0 -1.0471 TD
[(200)-224.2(\(kindly)-227.9(donate)-7.8(d)-217(b)0(y)-223.5(E.C.)-227.4(Butcher)25(,)-222.3(Stanfo)-8.2(rd)-219.4(Uni)-8.6(versity)]TJ
0 -1.0528 TD
[(School)-296.8(of)-287.7(Medic)-9(ine,)-290(Stanfo)-8.2(rd,)-288(CA,)-289.4(USA)-9.4(\))-286.9(o)0(r)-287.6(nonim)-9.3(mune)]TJ
0 -1.0471 TD
[(mous)-7.4(e)-274.3(IgG)-275.3(antibodies)-284.2(were)-276.8(used)-277.8(follow)-9.2(ed)-275.1(by)-274.7(fluorescei)-12.3(n)]TJ
0 -1.0528 TD
[(isothioc)-9.1(yanate)-8.2(-conj)-8.2(ugated)-332.1(goat)-324.5(an)-7.6(timouse)-333.5(seconda)-7.7(ry)-327.5(an-)]TJ
0 -1.0471 TD
[(tibody)-288.2(\(S)-8(igma\).)-290.6(Flow)-287.4(cytom)-9.7(etry)-286.3(was)-292.3(performed)-294.4(using)-291.9(an)]TJ
25.1977 66.1583 TD
[(Epi)-8.8(cs)]TJ
/F4 1 Tf
2.2022 0 TD
(1)Tj
/F11 1 Tf
1.0015 0 TD
[(-P)-8(rofile)-445.4(II)-448.6(\(Coult)-10(er)-441.7(Elec)-10.4(tronics\))-448.1(and)-446.6(resul)-9.4(ts)-443.3(are)]TJ
-3.2038 -1.0471 TD
[(exp)-8.4(ressed)-339.4(as)-333.2(mean)-337.1(fluor)-9.4(escence)-338.6(inte)-8.2(nsity)-339.8(of)-333.2(3,000)-339.2(cells.)]TJ
0 -3.1582 TD
[(In)-481.1(vitro)]TJ
/F3 1 Tf
3.642 0 TD
[(defor)-7.8(mability)-480.9(of)-476.2(5)]TJ
/F8 1 Tf
7.5627 0 TD
(')Tj
/F3 1 Tf
0.2219 0 TD
[(-br)29.1(omo-)-8.6(2)]TJ
/F8 1 Tf
3.7273 0 TD
(')Tj
/F3 1 Tf
0.2162 0 TD
[(-deoxyu)-8.1(ridine-l)-10.7(ab-)]TJ
-15.3702 -1.0471 TD
[(elle)-8.6(d)-330.8(polym)-10.5(orphonu)-6.5(clear)-338.2(leukoc)-8.2(ytes)]TJ
/F11 1 Tf
1.0015 -2.0998 TD
[(T)69.1(o)-302.4(te)12.8(st)-289.6(th)13.2(e)-302.8(d)0(e)20.8(f)0(o)13.9(r)0(m)16.5(a)0(b)20.8(i)0(l)16.5(i)0(t)16.5(y)-302.4(of)-299(B)11.6(r)0(d)13.9(U)12.4(-l)11.6(ab)20.8(el)18.5(le)18.4(d)-308.1(P)11.4(MN)15.3(L)-306.5(r)0(e)19.2(l)0(a)18.4(-)]TJ
-1.0015 -1.0527 TD
[(ti)16.5(ve)-235.2(to)-237.2(un)15.5(la)18.4(be)15.1(ll)16.5(ed)-235.2(P)11.4(M)0(N)15.3(L)0(,)-238.6(l)0(e)18.4(u)0(k)21.2(o)0(c)15.1(y)0(t)18.9(e)0(s)-236.4(w)0(e)16.9(r)0(e)-236.8(f)0(i)17.3(l)0(t)16.5(e)0(r)13.5(e)0(d)-235.2(t)0(h)18.8(r)0(o)19.6(u)0(g)15.5(h)]TJ
0 -1.0471 TD
[(po)15.5(ly)18.9(ca)14.7(rb)19.6(on)15.5(at)18.5(e)-354(f)0(i)17.3(l)0(t)16.5(e)0(r)19.2(s)-349.1(as)-344.6(pr)13.9(ev)20.8(io)18.9(us)14.4(ly)-345.3(de)20.8(sc)19.6(ri)11.6(be)20.8(d)-353.6([)0(3)19.6(0].)-356.3(Blood)]TJ
0 -1.0527 TD
[(samp)-7.8(les)-438.7(wer)-8.2(e)-439.3(obtained)-444.2(from)-441.9(the)-443.2(rabbits)-445.8(8)-433.3(h)-438.9(after)-442.7(they)]TJ
0 -1.0471 TD
[(wer)-8.2(e)-467.8(injected)-475.4(with)-472.7(either)-472.7(G-CSF)-473.4(or)-469.8(sali)-9.4(ne)-468.6(\(before)-471.9(the)]TJ
0 -1.0527 TD
[(pne)-8.4(umococci)-291.7(were)-288.2(inst)-9(illed)-284.5(in)-288.4(the)-289.6(lung\),)-287(and)-287.2(again)-291.5(at)-283.1(the)]TJ
T*
[(end)-315.7(of)-304.7(the)-312.4(study)-314.7(perio)-8.2(d)-308.1(\()0(2)-310.4(h)-308.1(after)-311.8(the)-312.4(pneum)-7.4(ococci)-315.5(were)]TJ
0 -1.0471 TD
[(inst)-9(illed)-386.9(into)-383.4(the)-386.3(lung\))-7.8(.)-381.6(LRP)-385.9(was)-383.4(p)-6.5(repared)-385.3(a)-6.9(s)-377.5(de)-7.6(scribed)]TJ
0 -1.0528 TD
[(abo)-8.4(ve,)-491.7(and)-497.8(filtered)]TJ
/F3 1 Tf
8.3879 0 TD
[(in)-499(vitr)30.4(o)]TJ
/F11 1 Tf
3.5964 0 TD
[(accordi)-10.5(ng)-490.9(to)-493.3(the)-500.2(filtration)]TJ
-11.9843 -1.0471 TD
[(met)-8.2(hod)-286.8(descri)-10.5(bed)-287.2(by)-286.1(L)]TJ
6.974 0 0 6.974 401.442 604.233 Tm
0.0001 Tc
[(ENNI)-11.5(E)]TJ
/F3 1 Tf
9.963 0 0 9.963 425.027 604.233 Tm
0 Tc
[(et)-288.8(al.)]TJ
/F11 1 Tf
2.3047 0 TD
[([31].)-291.1(Briefl)-12(y)61.8(,)-284.9(a)-285.7(20-m)-7.8(L)]TJ
-13.9191 -1.0528 TD
[(polypr)-8.6(opylen)-7.4(e)-251.6(syri)-8.2(nge)-253.1(\(Sherwood)-264(Medical)-258(Co.)-6.6(,)-250.8(S)0(t)-253.5(Louis,)]TJ
0 -1.0471 TD
[(MO)-7.4(,)-222.3(USA\))-228(was)-224.1(fil)-8.6(led)-221.3(wi)-7.8(th)-220.2(samp)-7.8(le)-226.2(solution)-230.7(\(LRP)101.1(,)-222.3(4)]TJ
/F9 1 Tf
20.3665 0 TD
(3)Tj
/F11 1 Tf
1.0584 0 TD
(10)Tj
7.472 0 0 6.641 532.743 587.735 Tm
(5)Tj
9.963 0 0 9.963 309.316 572.825 Tm
[(cell)-8.6(s)]TJ
/F10 1 Tf
1.8096 0.2049 TD
(.)Tj
/F11 1 Tf
0.2675 -0.2049 TD
(mL)Tj
7.472 0 0 6.641 343.729 577.247 Tm
(-1)Tj
9.963 0 0 9.963 349.909 572.825 Tm
[(\))-235.7(and)-241.7(filtered)-244.9(throu)-7.8(gh)-234.8(polyca)-7.8(rbonat)-9(e)-234.5(filters)-244.9(\(Po-)]TJ
-4.0744 -1.0471 TD
[(reti)-9.8(cs,)-572.6(Liverm)-10.5(ore,)-573.7(CA,)-579.6(USA\))-575.1(wi)-7.8(th)-573(defined)-579.2(pore)-574.1(size)]TJ
0 -1.0528 TD
[(\(pore)-297.6(diam)-9(eter)-292.4(5)]TJ
/F6 1 Tf
6.914 0 TD
(m)Tj
/F11 1 Tf
0.5747 0 TD
[(m;)-291.6(lengt)-9(h)-291(1)0(0)]TJ
/F6 1 Tf
5.4003 0 TD
(m)Tj
/F11 1 Tf
0.5747 0 TD
[(m)-288.4(and)-292.9(pore)-295.3(densi)-7.8(ty)-288.4(4)]TJ
/F9 1 Tf
8.5017 0 TD
(3)Tj
/F11 1 Tf
-21.9655 -1.0471 TD
(10)Tj
7.472 0 0 6.641 319.294 545.896 Tm
(5)Tj
/F10 1 Tf
9.963 0 0 9.963 323.036 543.458 Tm
(.)Tj
/F11 1 Tf
0.2731 -0.1992 TD
(cm)Tj
7.472 0 0 6.641 337.833 545.896 Tm
(-2)Tj
9.963 0 0 9.963 347.357 541.474 Tm
[(\(man)-8.2(ufact)-8.6(urer)]TJ
/F8 1 Tf
5.5995 0 TD
(')Tj
/F11 1 Tf
0.2162 0 TD
[(s)-343.4(data\))-8.6(\))-338.1(u)-6.5(sing)-342.4(a)-342.6(syri)-8.2(nge)-344.1(infusion)]TJ
-9.6341 -1.0528 TD
[(pump)-324.9(\(P)-8(ump)-324.1(22;)-323.3(Harvard)-326.2(Appa)-7.4(ratus,)-322.7(Mil)-9(lis,)-321.6(MA)-7.4(,)-319.1(USA\))]TJ
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[(whi)-8.6(ch)-223.9(provi)-7.8(ded)-230.3(a)-223.1(const)-7.8(ant)-227(flow)-232.9(rate)-229.8(\(3)-230.7(mL)]TJ
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[(th)18.9(e)-359.7(n)0(u)15.5(m)0(b)18.1(e)0(r)-356.3(o)0(f)-350.2(c)0(i)18.4(r)0(c)13.5(u)0(l)18.9(a)0(t)18.5(i)0(n)18.9(g)-359.3(PM)14.4(NL)-351(\(f)12.4(i)13.9(g)0(.)-354(2)0(\))-361.6(8)-370.7(h)-370.7(after)-374.4(treat)-11(-)]TJ
0 -1.0528 TD
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0 -1.0471 TD
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0 -1.0527 TD
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0 -1.0471 TD
[(percent)-10.5(age)-435.6(of)-435.6(PMNL)]TJ
7.472 0 0 6.641 145.417 470.721 Tm
(BrdU)Tj
9.963 0 0 9.963 166.224 466.299 Tm
[(in)-436.4(the)-437.6(circulati)-13.5(on)-434(\(fig.)-435.8(3\))-435.6(in-)]TJ
-10.8348 -1.0527 TD
[(creased)-373.9(signifi)-9.6(cantly)-368.7(from)-373.6(5.4\2132.1%)-370.7(to)-368.1(12.5\213)-8(3.1%)-369.2(with)]TJ
0 -1.0471 TD
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0 -1.0528 TD
[(with)-324.7(littl)-10(e)-319.8(furt)-8.6(her)-323(incre)-8.6(ase)-323(during)-327.2(the)-323.7(2)-319.4(h)]TJ
/F3 1 Tf
16.8554 0 TD
[(S.)-319.8(pneum)-8.2(oniae)]TJ
/F11 1 Tf
-16.8554 -1.0471 TD
[(pneum)-7.4(onia.)-472.8(In)-469.8(the)-477.4(saline)-472.3(contr)-8.2(ols,)-472.9(a)-473.5(small)-474.2(incre)-8.6(ase)-470.9(in)]TJ
0 -1.0528 TD
[(PMN)-7.4(L)]TJ
7.472 0 0 6.641 85.833 418.394 Tm
(BrdU)Tj
9.963 0 0 9.963 105.562 413.972 Tm
[(\(2.6\2130)-10.3(.9%)-322.9(to)-328.3(5.6\2132.3%\))-333.2(occurr)-8.6(ed)-320.6(d)-6.5(uring)-326.5(the)]TJ
-4.7459 -1.0471 TD
[(10-h)-238(study)-235(perio)-8.2(d)-234.1(\(fig.)-236.6(3\).)-236.8(The)-234.4(values)-242.3(in)-231.5(the)-238.4(G-CSF)-240.1(group)]TJ
0 -1.0527 TD
[(were)-259.8(signifi)-9.6(cantly)-260.5(higher)-259.3(than)-261.9(values)-259.3(in)-254.3(the)-261.2(control)-262.5(group)]TJ
0 -1.0471 TD
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/F6 1 Tf
18.0277 0 TD
(<)Tj
/F11 1 Tf
0.552 0 TD
(0.01\).)Tj
/F3 1 Tf
-18.5796 -2.151 TD
[(Expr)25.4(ession)-339.8(of)-334(CD18)-341.7(and)]TJ
6.974 0 0 6.974 161.688 361.19 Tm
(L)Tj
9.963 0 0 9.963 165.543 361.19 Tm
[(-sel)-8.6(ectin)]TJ
/F11 1 Tf
-9.765 -1.6503 TD
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6.974 0 0 6.974 194.91 344.75 Tm
0 Tc
(L)Tj
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-14.1296 -1.0527 TD
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25.1977 15.8027 TD
0.3487 Tc
[(f)348.7(o)362.6(l)348.7(l)365.2(o)348.7(w)366(i)348.7(n)367.6(gG)348.7(-)364.4(C)360.3(S)348.7(F)7.3(\()348.7(8)362.6(.)348.7(9)370.3(\213)348.7(2)365.4(.)348.7(4)370.3(%a)348.7(n)363.8(d)0.8(9)348.7(.)364.6(9)348.7(\213)371.1(0)348.7(.)364.6(4)348.7(%)367.6(\)a)348.7(n)363.8(d2hf)348.7(o)368.3(l)348.7(-)]TJ
0 -1.0471 TD
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0 -1.0527 TD
[(1)39.1(1)0(.)15.9(3)0(\213)16.7(0)0(.)21.6(6)0(%)13.2(\))-321(re)13.5(sp)20(ec)14.7(t)13.9(i)0(v)13.2(e)0(l)18.5(y)-325.1(di)18.9(d)-325.1(n)0(o)21.2(t)-327.5(ch)20.8(an)15.1(ge)20.8(.)]TJ
/F3 1 Tf
0 -3.1298 TD
[(Morph)-7.4(omet)-9.8(ry)-333.2(of)-334(lung)]TJ
/F11 1 Tf
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[(Th)17.1(e)-325.5(v)0(o)15.5(l)0(u)18.9(m)0(e)-318.1(o)0(f)-316.1(p)0(n)15.5(e)0(u)20.8(m)0(o)18.1(n)0(i)13.2(a)-325.5(i)13.9(n)-325.1(th)13.2(e)-325.5(G)12.4(-C)14.9(S)11.4(F)-330(gr)19.6(ou)15.5(p)-319.4(\()0(1)13.9(5)0(.)21.6(5)0(\213)]TJ
-1.0015 -1.0471 TD
[(2.)15.9(3)-268.2(m)0(L)14(\))-269.8(wa)16.9(s)-269.4(s)0(i)17.7(m)0(i)15.7(l)0(a)18.4(r)-275.5(to)-260(th)18.9(at)-260.4(in)-265.7(th)18.9(e)-268.6(c)0(o)15.1(n)0(t)18.9(r)0(o)19.6(l)-270.6(gr)13.9(ou)21.2(p)-273.9(\()0(1)19.6(2)0(.)15.9(5)0(\213)16.7(3)0(.)21.6(3)]TJ
0 -1.0528 TD
[(mL)14(\).)-196.2(Th)17.1(er)13.5(e)-206(w)0(e)16.9(r)0(e)-202.7(1)44.8(1\213)16.7(1.)15.9(7)-205.6(m)0(L)-202.3(o)0(f)-196.6(a)0(i)12.8(r)0(s)18.4(p)0(a)20.8(c)0(e)-201.5(a)0(n)15.1(d)-205.6(4.)21.6(2\213)16.7(0.)15.9(5)-205.6(m)0(L)-202.3(o)0(f)]TJ
0 -1.0471 TD
[(al)12.8(ve)20.8(ol)18.9(ar)-339.3(wa)16.9(ll)-336.3(in)-334(th)18.9(e)-348.3(p)0(n)21.2(e)0(u)15.1(m)0(o)18.1(n)0(i)18.9(c)-348.3(ar)19.2(ea)-338.1(in)-334(th)18.9(e)-348.3(G)12.4(-C)14.9(SF)-335.7(gr)19.6(ou)15.5(p)]TJ
ET
0 0 0 1 K
0 J 0 j 0.5 w 3.864 M []0 d
/GS3 gs
1 i 
87.03 190.602 m
87.03 313.851 l
87.03 313.851 m
84.78 313.851 l
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104.53 190.602 m
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122.53 190.602 m
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139.03 190.602 m
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156.28 190.602 m
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173.53 190.602 m
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242.779 190.602 m
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260.029 190.602 m
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277.529 190.602 m
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S
BT
/F19 1 Tf
10 0 0 10 73.78 309.101 Tm
0 0 0 1 k
/GS4 gs
(20)Tj
0.0061 -6.1444 TD
(10)Tj
0.45 -6.125 TD
(0)Tj
5.811 -0.875 TD
(1)Tj
-2.2018 0.0153 TD
[(0.25)-2374.8(2)-1305.8(5)-1269(8)]TJ
8.9564 -0.0038 TD
(12)Tj
1.694 0.0038 TD
(24)Tj
1.7255 -0.0178 TD
(72)Tj
3.3274 0.0178 TD
(220)Tj
-1.847 0 TD
(144)Tj
-15.1 0 TD
(0)Tj
7.8959 -1.322 TD
[(T)18(ime  h)]TJ
0 10 -10 0 68.571 227.352 Tm
(PMNL  )Tj
/F20 1 Tf
2.734 0 TD
( )Tj
/F19 1 Tf
0.25 0 TD
(10)Tj
0 8 -8 0 65.736 266.311 Tm
(9)Tj
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(\341L)Tj
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(-1)Tj
/F21 1 Tf
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(l)Tj
-4.3868 -8.5434 TD
(l)Tj
-4.375 -6.75 TD
(l)Tj
-4.268 -0.5985 TD
(l)Tj
-4.107 -0.714 TD
(l)Tj
-4.554 4.5 TD
(l)Tj
26.1165 20.8125 TD
(l)Tj
4.25 -5 TD
(l)Tj
4.3125 -16.6875 TD
(l)Tj
4.3125 2.5625 TD
(l)Tj
4.3125 -0.8125 TD
(l)Tj
ET
/GS3 gs
104.53 210.102 m
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S
BT
/F11 1 Tf
7.97 0 0 7.97 58.28 147.798 Tm
0 g
/GS2 gs
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/F6 1 Tf
27.158 0 TD
(m)Tj
/F11 1 Tf
0.5762 0 TD
(g)Tj
/F10 1 Tf
0.4979 0.249 TD
(.)Tj
/F11 1 Tf
-28.232 -1.3088 TD
(kg)Tj
5.977 0 0 5.313 66.217 142.866 Tm
(-1)Tj
7.97 0 0 7.97 71.15 139.351 Tm
[(\))-218.6(o)0(n)-216.3(the)-215.6(circulating)-218.3(polymor)11.9(phonuclear)-213.2(leukocyte)-210.4(\(PMNL\))-217.4(counts)-210.5(of)]TJ
-1.6147 -1.067 TD
[(rabbits)-232.3(\(n)]TJ
/F6 1 Tf
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(=)Tj
/F11 1 Tf
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[(3\).)-236.8(An)-233(initial)-238.1(transient)-236.5(drop)-233.6(\(within)-237(1)-232.7(h)0(\))-237.8(i)0(n)-240.1(PMNL)-228.4(counts)-238.9(was)]TJ
-4.2892 -1.0599 TD
[(followed)-309.7(by)-315.9(a)-317(steep)-311.1(rise)-314.4(that)-315.4(peaked)-311.6(at)-317.3(12)-308.8(h,)-316.9(at)-317.3(which)-306.3(point)-314.4(the)-315.2(counts)]TJ
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[(were)-333.7(six)-324.5(times)-336.2(the)-329.4(baseline)-327.5(levels.)-324.6(V)106.3(alues)-332.5(are)-333.3(mean\213)]TJ
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(SEM)Tj
7.97 0 0 7.97 239.811 113.896 Tm
(.)Tj
ET
/GS3 gs
390.566 671.881 m
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S
BT
/F19 1 Tf
10 0 0 10 330.566 770.831 Tm
0 0 0 1 k
/GS4 gs
(15)Tj
-0.0564 -4.2535 TD
(10)Tj
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(5)Tj
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(0)Tj
0.1 -1.3918 TD
(-24)Tj
5.1915 0.0091 TD
4.237 Tc
[(089)124.8(1)4237(0)]TJ
3.7575 -1.2852 TD
0 Tc
[(T)18(ime  h)]TJ
0 10 -10 0 319.748 685.043 Tm
(PMNL  10)Tj
0 8 -8 0 316.913 721.503 Tm
(9)Tj
0 10 -10 0 319.748 725.151 Tm
(\341L)Tj
0 8 -8 0 316.913 731.991 Tm
(-1)Tj
/F21 1 Tf
5 0 0 5 435.566 715.881 Tm
(l)Tj
-9.4377 -7.9887 TD
(l)Tj
18.95 9.5 TD
(l)Tj
9.35 -6.8 TD
(l)Tj
ET
/GS3 gs
532.315 691.131 m
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S
0 0 0 0 k
530.065 666.881 m
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530.849 665.131 530.065 665.914 530.065 666.881 c
b
483.002 665.687 m
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b
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439.002 682.971 438.219 682.187 437.252 682.187 c
436.286 682.187 435.502 682.971 435.502 683.937 c
b
388.752 671.937 m
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391.469 673.687 392.252 672.904 392.252 671.937 c
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b
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392.199 682.471 m
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S
BT
/F24 1 Tf
8 0 0 8 480.095 738.502 Tm
0 0 0 1 k
/GS4 gs
(*, #)Tj
-5.9687 -0.8125 TD
(*, #)Tj
12.3125 -3.4375 TD
(#)Tj
/F11 1 Tf
7.97 0 0 7.97 309.316 602.589 Tm
0 g
/GS2 gs
[(Fig.)-334.3(2.)-324.1(\261)-332.2(The)-329.7(eff)14.1(ect)-328.4(of)-330.3(granulocyt)11(e)-331.2(colony-)13.2(stimulating)-323.6(factor)-329.5(\(12.5)]TJ
/F6 1 Tf
27.1651 0 TD
(m)Tj
/F11 1 Tf
0.569 0 TD
(g)Tj
/F10 1 Tf
0.4979 0.249 TD
(.)Tj
/F11 1 Tf
-28.232 -1.3159 TD
(kg)Tj
5.977 0 0 5.313 317.31 597.656 Tm
(-1)Tj
7.97 0 0 7.97 322.186 594.085 Tm
[(\))-503.1(followed)-487.5(by)-500.8(pneumonia)-486.7(on)-493.7(the)-500.1(circulating)-495.7(polymor)11.9(phonuclear)]TJ
-1.6147 -1.0599 TD
[(leukocyte)-210.4(\(PMNL\))-203.2(counts)-210.5(\(n)]TJ
/F6 1 Tf
11.2885 0 TD
(=)Tj
/F11 1 Tf
0.5548 0 TD
[(6\).)-208.3(There)-210.8(was)-209.8(an)-215.3(increase)-203.4(in)-211.6(PMNL)-207.1(counts)]TJ
-11.8434 -1.0599 TD
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(-1)Tj
9.963 0 0 9.963 241.001 536.995 Tm
[(of)-401.5(airs)-8.6(pace)]TJ
-18.3406 -1.0527 TD
[(showing)-428.2(significant)-425.5(mig)-7.8(ration)-421.1(of)-424.2(PMNL.)-422.4(That)-419.7(was)-423.2(not)]TJ
0 -1.0471 TD
[(different)-387.1(from)-390.7(values)-395.9(in)-385.2(contr)-8.2(ol)-385.2(anim)-9(als)-387.5(\(27.2\2135)]TJ
/F9 1 Tf
20.3153 0 TD
(3)Tj
/F11 1 Tf
0.8365 0 TD
(10)Tj
7.472 0 0 6.641 278.986 520.497 Tm
(7)Tj
/F10 1 Tf
9.963 0 0 9.963 282.727 518.06 Tm
(.)Tj
/F11 1 Tf
-22.5289 -1.2519 TD
(mL)Tj
7.472 0 0 6.641 72 510.066 Tm
(-1)Tj
9.963 0 0 9.963 82.885 505.587 Tm
[(of)-469.7(airs)-8.6(pace\).)-479.3(V)103.4(ery)-476.6(few)-474.8(PMNL)-478.9(migrated)-483(into)-474.4(the)]TJ
-2.4697 -1.0471 TD
[(alveol)-10.1(ar)-225.5(space)-228.2(in)-220.2(the)-227(vehicl)-10.1(e-treated)-232.7(regio)-8.2(ns)-224.6(\(table)-228(1\).)-225.4(The)]TJ
0 -1.0528 TD
[(numbe)-7.4(r)-355.2(o)0(f)-355.9(PMNL)]TJ
7.472 0 0 6.641 131.357 489.09 Tm
(BrdU)Tj
9.963 0 0 9.963 151.37 484.668 Tm
[(in)-356.7(circu)-8.6(lating)-359.2(blood)-359(and)-355.5(lung)-358.2(are)]TJ
-9.3439 -1.0471 TD
[(shown)-253.6(in)-248.6(table)-248.4(2.)-245.8(Signi)-7.8(fican)-8.6(tly)-246.1(more)-252.9(PMN)-7.4(L)]TJ
7.472 0 0 6.641 232.044 478.658 Tm
(BrdU)Tj
9.963 0 0 9.963 251.036 474.236 Tm
(\(p)Tj
/F6 1 Tf
0.8194 0 TD
(<)Tj
/F11 1 Tf
0.5577 0 TD
(0.01\))Tj
-20.725 -1.0527 TD
[(were)-476(presen)-8.2(t)-469.8(i)0(n)-476.2(all)-474.1(lung)-472.1(regio)-8.2(ns)-469.3(in)-476.2(the)-471.7(G-CSF-)-9.1(treat)-11(ed)]TJ
0 -1.0471 TD
[(anima)-10.1(ls.)]TJ
1.0015 -1.0527 TD
[(Fi)19.6(gu)15.5(re)-271(4a)-263.7(show)-8.2(s,)-286.9(that)-287.1(in)-288.4(the)-289.6(G-CSF)-297(group,)-290.3(the)-289.6(percen-)]TJ
-1.0015 -1.0471 TD
[(tage)-455.8(of)-458.4(PMNL)]TJ
7.472 0 0 6.641 119.509 436.819 Tm
(BrdU)Tj
9.963 0 0 9.963 140.542 432.397 Tm
[(in)-453.5(the)-454.6(circu)-8.6(lating)-456(blood)-461.4(was)-457.4(signi-)]TJ
-8.257 -1.0528 TD
[(fican)-8.6(tly)-314.3(low)-8.6(er)-316.5(than)-318.8(the)-318.1(percent)-10.5(age)-321.8(of)-316.1(PMN)-7.4(L)]TJ
7.472 0 0 6.641 237.26 426.331 Tm
(BrdU)Tj
9.963 0 0 9.963 256.932 421.909 Tm
[(in)-316.9(lung)]TJ
-19.9397 -1.0471 TD
[(microvess)-11.5(els)-222.5(at)-226.2(the)-221.3(pneum)-7.4(onic)-222.1(sit)-8.2(e,)-217.8(as)-225.1(well)-222.7(as)-225.1(the)-221.3(vehicl)-10.1(e-)]TJ
0 -1.0528 TD
[(treated)-416.9(or)-412.8(untreated)-418.5(lung)-409.5(regio)-8.2(ns)-406.7(\(p)]TJ
/F6 1 Tf
14.7499 0 TD
(<)Tj
/F11 1 Tf
0.552 0 TD
[(0)-6.5(.05\).)-408.7(The)-410.8(contr)-8.2(ol)]TJ
-15.3019 -1.0471 TD
[(group)-221.7(show)-8.2(ed)-218.2(simila)-10.8(r)-218.6(dif)10.8(ference)-10.1(s)-218.2(\(data)-224.8(not)-220.9(shown\).)-222.2(The)-6.8(se)]TJ
0 -1.0528 TD
[(findings)-275.4(are)-266.5(compa)-7.8(tible)-267.4(with)-267.8(prefer)-10.2(enti)-8.2(al)-266.1(sequestrat)-11.9(ion)-266.4(of)]TJ
0 -1.0471 TD
[(PMN)-7.4(L)]TJ
7.472 0 0 6.641 85.833 374.06 Tm
(BrdU)Tj
9.963 0 0 9.963 104.995 369.638 Tm
[(in)-271.4(the)-266.8(infec)-8.6(ted)-266.8(and)-270.2(noninf)-8.6(ected)-269.2(regio)-8.2(ns)-264.5(of)-270.6(the)]TJ
-4.689 -1.0527 TD
[(lung)-335.5(in)-339.7(both)-335.5(groups)-7.4(.)]TJ
1.0015 -1.0471 TD
[(Fi)19.6(gu)15.5(re)-367.7(4b)-360(show)-8.2(s)-383.2(the)-386.3(percent)-10.5(age)-384.4(of)-384.4(PMN)-7.4(L)]TJ
7.472 0 0 6.641 241.512 353.14 Tm
(BrdU)Tj
9.963 0 0 9.963 261.865 348.718 Tm
[(in)-385.2(the)]TJ
-20.4348 -1.0527 TD
[(alveol)-10.1(ar)-288.1(walls)-292.9(and)-287.2(alveol)-10.1(ar)-288.1(airs)-8.6(pace)-288.8(in)-288.4(the)-289.6(G-CSF)-297(group.)]TJ
0 -1.0471 TD
[(There)-431.4(were)-430.5(approxi)-9.7(mately)-434.4(5)-421.9(times)-434.4(more)-429.3(PMN)-7.4(L)]TJ
7.472 0 0 6.641 256.989 332.22 Tm
(BrdU)Tj
9.963 0 0 9.963 277.739 327.798 Tm
(in)Tj
-22.0281 -1.0527 TD
[(alveol)-10.1(ar)-356.3(wal)-9(ls)-357.9(than)-364.3(in)-362.4(alveolar)-366.5(spaces)-366.7(in)-356.7(the)-363.6(pneum)-7.4(onia)]TJ
ET
1 i 
57.827 282.047 227.679 -0.454 re
f
126.709 267.137 79.257 -0.283 re
f
201.713 267.137 83.792 -0.283 re
f
57.827 246.727 227.679 -0.51 re
f
57.827 192.416 227.679 -0.51 re
f
BT
/F2 1 Tf
8.966 0 0 8.966 57.827 295.88 Tm
[(Table)-331.9(1.)-336.7(\261)-908.6(Total)-571.3(number)-577.7(of)-570.7(polymorphonuclear)-573.7(leuko-)]TJ
0 -1.0559 TD
[(cytes)-335.2(\(PMNL\))-333.3(in)-330.2(the)-334.8(lungs)]TJ
/F11 1 Tf
0 -1.7072 TD
[(Regions)-6112.4(G-CSF)-332.4(group)-3039.7(Control)-335.7(group)]TJ
8.0744 -2.1118 TD
[(Alv-walls)-232.4(Alv-space)-483.1(Alv-walls)-491.6(Alv-space)]TJ
-8.0744 -1.998 TD
(Lung)Tj
0.3351 -1.0559 TD
[(Pneumonic)-3197.2(25.6\2134.3)-891.2(39.5\2137)-1173.4(1)32.1(1.3\2132.2)]TJ
6.725 0 0 5.977 236.296 228.756 Tm
(#)Tj
8.966 0 0 8.966 248.825 224.787 Tm
(27.2\2135)Tj
-20.9668 -1.0559 TD
[(V)104.7(ehicle-treated)-1703.8(17.6\2133)-2140.8(3.5\2130.6)-891.7(6.9\2131.4)]TJ
6.725 0 0 5.977 236.296 219.288 Tm
(#)Tj
8.966 0 0 8.966 253.304 215.32 Tm
(1.1\2130.5)Tj
-21.4663 -1.0559 TD
[(Untreated)-3772.2(13.2\2131.2)]TJ
6.725 0 0 5.977 161.291 209.82 Tm
(+)Tj
8.966 0 0 8.966 209.027 205.852 Tm
(3.4\2131.7)Tj
6.725 0 0 5.977 236.296 209.82 Tm
0.0001 Tc
[(#,)-8.6(+)]TJ
8.966 0 0 8.966 57.827 196.384 Tm
0 Tc
[(Circulating)-337.9(blood)-948.3(0.52\2130.09)-4324(0.24\2130.02*)]TJ
0 -1.9411 TD
[(The)-298.9(numbers)-303.7(are)-296.2(PMNL)]TJ
/F9 1 Tf
9.8132 0 TD
(3)Tj
/F11 1 Tf
0.8346 0 TD
(10)Tj
6.725 0 0 5.977 162.255 182.948 Tm
(7)Tj
/F10 1 Tf
8.966 0 0 8.966 165.6 180.964 Tm
(.)Tj
/F11 1 Tf
0.2719 -0.2213 TD
(mL)Tj
6.725 0 0 5.977 180.34 182.948 Tm
(-1)Tj
8.966 0 0 8.966 188.617 178.979 Tm
[(of)-297.1(alveolar)-304.5(wall)-296.7(\(Alv-wall\))]TJ
-14.587 -1.0559 TD
[(or)-373(alveolar)-374(space)-371(\(Alv-space\).)-379.4(Granulocyte)-375.8(colony-stimulating)]TJ
T*
[(factor)-380.2(\(G-CSF\))-377.5(group:)-379.7(n)]TJ
/F6 1 Tf
9.9712 0 TD
(=)Tj
/F11 1 Tf
0.5501 0 TD
[(6,)-375.5(control)-375(group:)-379.7(n)]TJ
/F6 1 Tf
7.7709 0 TD
(=)Tj
/F11 1 Tf
0.5501 0 TD
[(3.)-375.5(*:)-380.7(circulating)]TJ
-18.8423 -1.0559 TD
[(PMNL)-484.6(counts)-480.8(were)-488.6(lower)-484.4(in)-481.9(the)-487.5(control)-482.5(group)-485.3(\(p)]TJ
/F6 1 Tf
21.3082 0 TD
(<)Tj
/F11 1 Tf
0.5501 0 TD
(0.05\);)Tj
6.725 0 0 5.977 279.156 154.545 Tm
(#)Tj
8.966 0 0 8.966 282.557 150.576 Tm
(:)Tj
-25.064 -1.0559 TD
[(PMNL)-212.7(counts)-208.9(in)-210(all)-211.7(three)-215.3(lung-regions)-210.2(in)-210(the)-209.3(alveolar)-216(walls)-215.1(were)]TJ
T*
[(lower)-427.5(in)-425(the)-424.3(control)-425.6(group)-422.1(\(p)]TJ
/F6 1 Tf
12.2917 0 TD
(<)Tj
/F11 1 Tf
0.5564 0 TD
(0.05\);)Tj
6.725 0 0 5.977 197.802 135.609 Tm
(+)Tj
8.966 0 0 8.966 201.6 131.641 Tm
[(:)-425.5(PMNL)-421.3(counts)-430.3(in)-425(the)]TJ
-16.0349 -1.0559 TD
[(untreated)-285(lung)-284.9(region)-284.6(were)-286.3(lower)-288.4(than)-284.7(in)-285.9(the)-285.2(pneumonic)-283.7(region)]TJ
T*
(\(p)Tj
/F6 1 Tf
0.822 0 TD
(<)Tj
/F11 1 Tf
0.5501 0 TD
(0.04\).)Tj
ET
309.316 749.367 227.679 -0.51 re
f
358.072 734.4 82.885 -0.283 re
f
435.855 734.4 101.14 -0.283 re
f
309.316 713.99 227.679 -0.51 re
f
309.316 640.743 227.679 -0.454 re
f
BT
/F2 1 Tf
8.966 0 0 8.966 309.316 772.667 Tm
[(Table)-338.2(2.)-330.4(\261)-1009.8(Total)-678.8(number)-672.5(of)-678.2(5)]TJ
/F8 1 Tf
14.5553 0 TD
(')Tj
/F2 1 Tf
0.2213 0 TD
(-bromo-2)Tj
/F8 1 Tf
4.0403 0 TD
(')Tj
/F2 1 Tf
0.215 0 TD
(-deoxyuridine-)Tj
-19.032 -1.0559 TD
[(labelled)-638.9(polymorphonuclear)-630.7(leukocytes)-635.7(\(PMNL\))-630.5(in)-633.7(the)]TJ
T*
(lungs)Tj
/F11 1 Tf
0 -1.7072 TD
[(Regions)-4070.1(G-CSF)-332.4(group)-4114.6(Control)-335.7(group)]TJ
6.0573 -2.1119 TD
[(Alv-walls)-390.5(Alv-space)-906.7(Alv-walls)-1408.4(Alv-space)]TJ
-6.0573 -2.0043 TD
(Lung)Tj
0.3351 -1.0559 TD
[(Pneumonic)-1667(13\2132.08)-1065.4(4.4\2131)-2312(2.8\2130.5)]TJ
6.725 0 0 5.977 474.916 696.075 Tm
(#)Tj
8.966 0 0 8.966 497.877 692.05 Tm
(2.6\2130.9)Tj
-20.6949 -1.0496 TD
[(V)104.7(ehicle-)]TJ
0.3351 -1.0559 TD
(treated)Tj
5.5515 1.0559 TD
(6.8\2131.2)Tj
6.725 0 0 5.977 392.372 686.608 Tm
(+)Tj
8.966 0 0 8.966 401.896 682.639 Tm
[(0.08\2130.01)-1061.4(1.2\2130.3)]TJ
6.725 0 0 5.977 474.916 686.608 Tm
0.0001 Tc
(#,+)Tj
8.966 0 0 8.966 488.92 682.639 Tm
0 Tc
(0.009\2130.001)Tj
-19.6959 -2.1119 TD
[(Untreated)-1995.5(4.9\2131.5)]TJ
6.725 0 0 5.977 392.372 667.672 Tm
(+)Tj
8.966 0 0 8.966 443.168 663.704 Tm
(0.49\2130.04)Tj
6.725 0 0 5.977 479.395 667.672 Tm
0.0001 Tc
(#,+)Tj
8.966 0 0 8.966 309.316 654.236 Tm
0 Tc
(Circulating)Tj
0.6702 -1.0559 TD
(blood)Tj
5.0836 1.0559 TD
[(0.1)36(1\2130.002)-4169.9(0.012\2130.003*)]TJ
-5.7538 -3.0034 TD
[(The)-311.6(numbers)-310.1(are)-302.5(PMNL)]TJ
6.725 0 0 5.977 397.531 631.275 Tm
0.0001 Tc
(BrdU)Tj
/F9 1 Tf
8.966 0 0 8.966 415.162 627.307 Tm
0 Tc
(3)Tj
/F11 1 Tf
0.8346 0 TD
(10)Tj
6.725 0 0 5.977 431.603 631.275 Tm
(7)Tj
/F10 1 Tf
8.966 0 0 8.966 434.948 629.291 Tm
(.)Tj
/F11 1 Tf
0.2719 -0.2213 TD
(mL)Tj
6.725 0 0 5.977 449.688 631.275 Tm
(-1)Tj
8.966 0 0 8.966 458.022 627.307 Tm
[(of)-303.5(alveolar)-310.8(wall)-309.3(\(Alv-)]TJ
-16.585 -1.0559 TD
[(wall\))-297.1(or)-297.1(alveolar)-298.2(space)-295.1(\(Alv-space\).)-297.2(Granulocyte)-299.9(colony-stimu-)]TJ
T*
[(lating)-623.5(factor)-626.8(\(G-CSF\))-624.1(group:)-626.3(n)]TJ
/F6 1 Tf
13.5816 0 TD
(=)Tj
/F11 1 Tf
0.5501 0 TD
[(6,)-622.1(control)-621.6(group:)-626.3(n)]TJ
/F6 1 Tf
8.5106 0 TD
(=)Tj
/F11 1 Tf
0.5501 0 TD
[(3.)-622.1(*:)]TJ
-23.1924 -1.0559 TD
[(circulating)-364.6(PMNL)]TJ
6.725 0 0 5.977 375.08 602.929 Tm
0.0001 Tc
(BrdU)Tj
8.966 0 0 8.966 393.109 598.904 Tm
0 Tc
[(counts)-360.7(were)-355.9(lower)-358(in)-349.1(the)-361(control)-356(group)]TJ
-9.3453 -1.0559 TD
(\(p)Tj
/F6 1 Tf
0.8283 0 TD
(<)Tj
/F11 1 Tf
0.5501 0 TD
(0.05\);)Tj
6.725 0 0 5.977 345.94 593.461 Tm
(#)Tj
8.966 0 0 8.966 349.285 589.436 Tm
[(:)-362.3(PMNL)]TJ
6.725 0 0 5.977 379.672 593.461 Tm
0.0001 Tc
(BrdU)Tj
8.966 0 0 8.966 397.814 589.436 Tm
0 Tc
[(counts)-360.7(in)-361.8(all)-363.4(three)-367.1(lung-regions)-361.9(in)-361.8(the)]TJ
-9.8701 -1.0559 TD
[(alveolar)-494.2(walls)-493.4(were)-488.6(lower)-490.7(in)-488.2(the)-487.5(control)-495.1(group)-485.3(\(p)]TJ
/F6 1 Tf
21.2576 0 TD
(<)Tj
/F11 1 Tf
0.5564 0 TD
(0.05\);)Tj
6.725 0 0 5.977 530.249 583.994 Tm
(+)Tj
8.966 0 0 8.966 534.047 579.968 Tm
(:)Tj
-25.064 -1.0496 TD
(PMNL)Tj
6.725 0 0 5.977 334.091 574.526 Tm
0.0001 Tc
(BrdU)Tj
8.966 0 0 8.966 351.666 570.557 Tm
0 Tc
[(counts)-297.5(in)-304.9(both)-297.5(the)-297.8(vehicle-treated)-306(and)-301.8(the)-304.1(untreated)]TJ
-4.7232 -1.0559 TD
[(lung)-240.6(regions)-241(were)-242(lower)-244.1(than)-240.4(in)-235.3(the)-240.9(pneumonic)-239.5(region)-240.3(\(p)]TJ
/F6 1 Tf
22.459 0 TD
(<)Tj
/F11 1 Tf
0.5564 0 TD
(0.04\).)Tj
ET
0 0 0 1 K
0 J 0 j 0.48 w 3.864 M []0 d
/GS3 gs
347.698 405.183 m
347.698 525.182 l
347.698 525.182 m
345.778 525.182 l
347.218 494.702 m
345.778 494.702 l
347.458 464.462 m
345.778 464.462 l
347.458 434.942 m
345.778 434.942 l
345.778 405.183 m
532.737 405.183 l
382.737 405.183 m
382.737 403.263 l
419.217 405.183 m
419.217 403.263 l
455.697 405.183 m
455.697 403.263 l
491.457 405.183 m
491.457 403.263 l
365.457 434.702 36.24 -29.52 re
401.697 479.582 36.48 -74.4 re
438.177 461.342 36.48 -56.16 re
474.657 454.382 37.2 -49.2 re
493.377 462.062 m
493.377 454.622 l
456.177 472.382 m
456.177 461.342 l
420.657 492.302 m
420.657 479.582 l
383.937 442.862 m
383.937 435.182 l
465.057 472.622 m
447.777 472.622 l
392.756 443.396 m
375.476 443.396 l
429.106 492.356 m
411.826 492.356 l
502.196 462.116 m
484.916 462.116 l
S
BT
/F24 1 Tf
7.68 0 0 7.68 419.217 495.278 Tm
0 0 0 1 k
/GS4 gs
(*)Tj
4.6327 -2.618 TD
(*)Tj
4.8125 -1.4375 TD
(*)Tj
/F19 1 Tf
9.6 0 0 9.6 334.978 521.647 Tm
(80)Tj
-0.0064 -3.1468 TD
(60)Tj
0 -3.1817 TD
(40)Tj
0 -3.0784 TD
(20)Tj
0.475 -3.1033 TD
(0)Tj
10.7582 -0.9783 TD
[(V)45(ehicle-)]TJ
0.1821 -1 TD
(treated)Tj
3.2101 1.0091 TD
(Untreated)Tj
-7.7452 0.0034 TD
(Pneumonia)Tj
-2.8503 -0.0034 TD
(Blood)Tj
ET
/GS3 gs
347.586 262.779 m
347.586 379.983 l
345.666 262.779 m
532.625 262.779 l
382.626 262.779 m
382.626 260.859 l
419.105 262.779 m
419.105 260.859 l
455.585 262.779 m
455.585 260.859 l
492.728 262.779 m
492.728 260.859 l
347.287 280.143 m
345.367 280.143 l
347.287 296.703 m
345.367 296.703 l
347.287 313.503 m
345.367 313.503 l
347.287 330.303 m
345.367 330.303 l
347.586 379.983 m
345.367 379.983 l
347.287 363.663 m
345.367 363.663 l
347.287 347.103 m
345.367 347.103 l
365.527 347.274 35.76 -84.48 re
401.287 281.343 36 -18.549 re
437.286 316.143 36.48 -53.349 re
473.766 264.783 36 -1.989 re
456.21 323.103 m
456.21 316.383 l
419.766 285.903 m
419.766 281.343 l
384.007 360.783 m
384.007 347.103 l
392.887 361.263 m
374.887 361.263 l
428.705 286.437 m
410.706 286.437 l
465.149 323.637 m
447.149 323.637 l
501.425 266.277 m
483.425 266.277 l
492.006 265.983 m
492.006 265.023 l
S
BT
/F24 1 Tf
7.68 0 0 7.68 417.665 291.093 Tm
/GS4 gs
(#)Tj
9.5 -2.5 TD
(#)Tj
/F19 1 Tf
9.6 0 0 9.6 363.86 249.924 Tm
(Capillaries)Tj
4.3826 0.0045 TD
(Airspace)Tj
3.4898 -0.0023 TD
(Capillaries)Tj
4.2576 0.0045 TD
(Airspace)Tj
-10.3169 -1.7045 TD
(Pneumonia)Tj
0.9342 -1 TD
(region)Tj
6.127 0.9909 TD
[(V)45(ehicle-treated)]TJ
1.5947 -1 TD
(region)Tj
ET
/GS3 gs
365.287 244.863 m
435.366 244.863 l
440.646 244.863 m
510.966 244.863 l
S
BT
9.6 0 0 9.6 334.917 376.717 Tm
/GS4 gs
(70)Tj
0 -1.7034 TD
(60)Tj
0 -1.7783 TD
(50)Tj
0.0038 -1.7283 TD
(40)Tj
-0.0038 -1.75 TD
(30)Tj
0 -1.7467 TD
(20)Tj
0 -1.725 TD
(10)Tj
0.475 -1.7784 TD
(0)Tj
-2.55 12.0683 TD
(b\))Tj
-0.025 15.2 TD
(a\))Tj
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[(il)16.5(it)16.5(y)-342.2(o)0(f)-333.2(P)11.4(MN)15.3(L)-340.6(t)0(o)-334(s)0(e)19.6(q)0(u)15.5(e)0(s)19.6(t)0(e)18.4(r)-343.8(in)-334(lu)18.8(ng)-331.6(mi)15.7(cr)19.2(ov)15.5(es)19.6(se)19.6(ls)-335.2(an)15.1(d)-336.5(m)0(i)15.7(-)]TJ
0 -1.0528 TD
[(gr)13.9(at)18.5(e)-359.7(i)0(n)18.9(t)0(o)-356.7(t)0(h)18.9(e)-359.7(al)18.5(ve)15.1(ol)18.9(us)-349.8(du)15.5(ri)17.3(ng)-354.3(pn)21.2(eu)15.1(mo)18.1(co)20.8(cc)14.7(al)-351.4(pn)15.5(eu)20.8(mo)12.4(ni)18.9(a.)]TJ
T*
[(Th)17.1(e)-302.8(r)0(e)19.2(s)0(u)14.4(l)0(t)16.5(s)-297.9(sh)14.4(ow)-290(th)18.9(at)-294.5(G-)15.7(CS)17.3(F)-301.6(r)0(a)19.2(p)0(i)13.2(d)0(l)18.9(y)-302.4(re)19.2(le)18.4(as)13.9(e)-297.1(P)0(M)14.4(N)0(L)-294.1(f)0(r)18(o)0(m)]TJ
0 -1.0471 TD
[(th)13.2(e)-291.4(b)0(o)15.5(n)0(e)-286.5(m)0(a)17.7(r)0(r)18(o)0(w)-290(a)0(n)20.8(d)-296.7(in)18.9(cr)19.2(ea)14.7(se)-287.7(th)18.9(e)-291.4(t)0(o)13.2(t)0(a)18.4(l)-293.4(nu)15.5(mb)18.1(er)-288.1(of)-282(PM)14.4(NL)]TJ
0 -1.0527 TD
[(pr)13.9(es)19.6(en)15.1(t)-270.6(i)0(n)-260(t)0(h)13.2(e)-268.6(lu)18.8(ng)-263.3(co)15.1(mp)18.1(ar)19.2(ed)-263.7(to)-265.7(co)20.8(nt)18.9(ro)13.9(ls)17.7(.)-267.8(T)0(h)17.1(e)-274.3(ne)20.8(w)-272.2(P)0(M)14.4(N)0(L)]TJ
0 -1.0471 TD
[(re)13.5(le)18.4(as)19.6(ed)-286.5(fr)12.4(om)-289.2(t)13.9(h)0(e)-292.2(b)0(o)21.2(n)0(e)-286.5(m)0(a)12(r)0(r)18(o)0(w)-284.3(p)0(r)13.9(e)0(f)19.2(e)0(r)19.2(e)0(n)15.1(t)0(i)16.5(a)0(l)18.5(l)0(y)-288.4(s)0(e)19.6(q)0(u)15.5(e)0(s)19.6(t)0(e)18.4(r)-298.3(in)]TJ
0 -1.0527 TD
[(mi)15.7(cr)13.5(ov)21.2(es)13.9(se)19.6(ls)-295.3(in)-294.1(bo)15.5(th)-294.1(th)18.9(e)-302.8(i)0(n)18.8(f)0(e)13.5(c)0(t)18.4(e)0(d)-292.2(a)0(n)15.1(d)-302.4(no)15.5(ni)18.9(nf)19.6(ec)14.7(te)18.4(d)-302.4(r)0(e)19.2(g)0(i)13.2(o)0(n)21.2(s)]TJ
0 -1.0471 TD
[(of)-208(th)18.9(e)-211.7(l)0(u)18.8(n)0(g)15.5(.)-210.9(Th)17.1(es)13.9(e)-211.7(n)0(e)20.8(w)0(l)14.9(y)-211.3(re)13.5(le)18.4(as)19.6(ed)-206.8(P)11.4(M)0(N)15.3(L)-215.4(we)16.9(re)-202.7(al)12.8(so)-201.9(sl)17.7(ow)-204.6(to)]TJ
0 -1.0527 TD
[(mi)15.7(gr)13.9(at)18.5(e)-280(i)0(n)18.9(t)0(o)-271.4(t)0(h)18.8(e)-280(al)18.5(ve)20.8(ol)13.2(us)20(.)-279.2(T)12.2(he)-275.1(au)20.8(th)18.8(or)13.9(s)-275.1(s)0(p)14.4(e)0(c)20.4(u)0(l)18.9(a)0(t)12.8(e)-274.3(th)13.2(at)-266.1(th)13.2(es)19.6(e)]TJ
0 -1.0471 TD
[(PM)14.4(NL)-271.3(ar)19.2(e)-280(s)0(t)17.7(r)0(u)19.6(c)0(t)12.8(u)0(r)19.6(a)0(l)18.4(l)0(y)-271.4(a)0(n)20.8(d)-279.6(fu)19.6(nc)15.1(ti)16.5(on)21.2(al)12.8(l)13.9(y)-279.6(st)17.7(il)16.5(l)-276.3(i)0(m)15.7(m)0(a)12(t)13.9(ur)14(e,)-269(re)19.2(-)]TJ
0 -1.0528 TD
[(su)14.4(lt)16.5(in)18.8(g)-393.4(i)13.9(n)-393.4(p)0(r)13.9(e)0(f)19.2(e)0(r)19.2(e)0(n)15.1(t)0(i)16.5(a)0(l)-379.9(s)0(e)14(q)0(u)21.2(e)0(s)13.9(t)0(r)17.3(a)0(t)18.4(i)0(o)18.8(n)-393.4(in)-379.5(lu)13.2(ng)-382.8(mi)15.7(cr)19.2(ov)21.2(es)13.9(se)19.6(ls)]TJ
0 -1.0471 TD
[(an)15.1(d)-325.1(a)0(n)-314.9(i)0(n)18.9(a)0(b)15.1(i)0(l)22.2(i)0(t)16.5(y)-325.1(to)-316.9(mi)15.7(gr)19.6(at)18.5(e)-325.5(i)0(n)18.9(t)0(o)-316.9(a)0(n)-314.9(i)0(n)18.8(f)0(e)13.5(c)0(t)18.4(i)0(o)18.9(u)0(s)-315.7(s)0(i)17.7(t)0(e)18.5(.)]TJ
1.0015 -1.0527 TD
[(T)69.1(o)-325.1(te)18.4(st)-318.1(th)18.9(e)-325.5(h)0(y)15.5(p)0(o)21.2(t)0(h)18.9(e)0(s)13.9(i)0(s)-312.4(t)0(h)13.2(a)0(t)-311.6(G)0(-)15.7(C)0(S)17.3(F)-324.3(ch)15.1(an)20.8(ge)15.1(s)-326.3(t)0(h)18.9(e)-325.5(se)19.6(qu)15.5(es)19.6(-)]TJ
-1.0015 -1.0471 TD
[(tr)17.3(at)12.8(io)18.9(n)-421.9(a)0(n)15.1(d)-421.9(mi)15.7(gr)19.6(at)12.8(i)13.9(o)0(n)-416.9(o)0(f)-418.5(P)11.4(MN)15.4(L)-426(r)0(e)19.2(l)0(e)18.4(a)0(s)13.9(e)0(d)-411.7(f)0(r)18(o)0(m)-420.1(t)0(h)18.8(e)-422.3(bo)15.5(ne)]TJ
0 -1.0527 TD
[(ma)12(rr)18(ow)79.9(,)-398.7(t)0(h)18.9(e)-393.8(au)15.1(th)18.9(or)13.9(s)-394.6(d)0(e)15.1(c)0(i)18.4(d)0(e)20.8(d)-399.1(to)-390.9(st)17.7(ud)21.2(y)-399.1(l)0(u)18.9(n)0(g)-388.5(s)0(e)13.9(q)0(u)21.2(e)0(s)13.9(t)13.9(r)0(a)13.5(t)0(i)16.5(o)0(n)]TJ
T*
[(an)15.1(d)-404.8(m)13.2(ig)13.2(ra)19.2(ti)16.5(on)-394.2(of)-390.1(th)13.2(es)19.6(e)-405.2(c)0(e)20.4(l)0(l)16.5(s)-400.3(10)-394.2(h)-404.8(a)0(f)19.2(t)0(e)18.4(r)-406.4(G)12.4(-)0(C)14.9(S)0(F)-392.6(a)0(d)15.1(m)0(i)15.7(n)0(i)18.8(s)0(-)]TJ
0 -1.0471 TD
[(tr)17.3(at)12.8(io)18.9(n)-438.9(a)0(n)20.8(d)-444.6(2)-438.9(h)-438.9(f)0(o)19.6(l)0(l)16.5(o)0(w)17.3(i)0(n)18.9(g)-438.9(an)-434.4(i)13.9(n)0(f)13.9(l)0(a)18.5(m)0(m)14.9(a)0(t)18.5(o)0(r)13.9(y)-438.9(st)17.7(im)15.7(ul)18.9(us)-429.5(in)18.8(-)]TJ
0 -1.0527 TD
[(st)17.7(il)16.5(le)12.8(d)-211.3(i)0(n)-203.1(t)0(h)18.8(e)-217.4(lu)18.9(ng)15.5(.)-210.9(T)0(h)17.1(e)-211.7(re)13.5(as)19.6(on)15.5(in)18.8(g)-211.3(f)0(o)13.9(r)-212.9(th)18.8(is)-204.3(ap)15.1(pr)19.6(oa)15.1(ch)-206.8(w)12.4(a)0(s)-208(t)0(h)18.9(a)0(t)]TJ
0 -1.0471 TD
[(mi)15.7(gr)13.9(at)18.5(io)18.9(n)-387.7(o)0(f)-378.7(P)11.4(MN)15.3(L)-391.8(f)0(r)18(o)0(m)-380.3(t)0(h)18.9(e)-388.1(pu)21.2(lm)15.7(on)15.5(ar)19.2(y)-387.7(c)0(a)14.7(p)0(i)18.9(l)0(l)16.5(a)0(r)19.2(i)0(e)18.4(s)-388.9(in)18.8(to)]TJ
0 -1.0527 TD
[(th)13.2(e)-280(a)0(l)18.4(v)0(e)20.8(o)0(l)13.2(a)0(r)-271(s)0(p)20(a)0(c)20.4(e)-280(oc)15.1(cu)20.8(rs)-271.8(ea)14.7(rl)17.3(y)-279.6(\()0(1)19.6(\261)0(2)-269(h)0(\))-270.6(f)0(o)13.9(l)0(l)16.5(o)0(w)17.3(i)0(n)18.9(g)]TJ
/F3 1 Tf
19.5584 0 TD
[(S.)-268.6(pn)15.5(eu)20.8(-)]TJ
-19.5584 -1.0471 TD
[(mo)17.3(ni)13.2(ae)]TJ
/F11 1 Tf
3.1696 0 TD
[(in)18.9(st)17.7(il)16.5(la)18.4(ti)16.5(on)-274.7(in)-271.4(ra)13.5(bb)21.2(it)16.5(s)-286.5([)0(2)19.6(3].)-288(This)-295.3(was)-298(suppor)-7.4(ted)-295.3(by)]TJ
-3.1696 -1.0527 TD
[(the)-352.2(study)-354.5(\(mi)-9.4(gration)-353.5(39.5\213)-8(7)]TJ
/F9 1 Tf
11.3925 0 TD
(3)Tj
/F11 1 Tf
0.8308 0 TD
(10)Tj
7.472 0 0 6.641 441.127 545.896 Tm
(7)Tj
/F10 1 Tf
9.963 0 0 9.963 444.812 543.458 Tm
(.)Tj
/F11 1 Tf
0.2732 -0.1992 TD
(mL)Tj
7.472 0 0 6.641 461.253 545.896 Tm
(-1)Tj
9.963 0 0 9.963 470.891 541.474 Tm
[(in)-351(G-CSF)-359.6(27.2\213)]TJ
-16.2181 -1.0471 TD
(5)Tj
/F9 1 Tf
0.5008 0 TD
(3)Tj
/F11 1 Tf
0.8365 0 TD
(10)Tj
7.472 0 0 6.641 332.617 535.464 Tm
(7)Tj
/F10 1 Tf
9.963 0 0 9.963 336.359 533.027 Tm
(.)Tj
/F11 1 Tf
0.2732 -0.1992 TD
(mL)Tj
7.472 0 0 6.641 352.8 535.464 Tm
(-1)Tj
9.963 0 0 9.963 361.757 531.042 Tm
[(in)-282.7(contr)-8.2(ol)-282.7(group)-284.3(at)-283.1(the)-289.6(2-h)-282.7(time)-284.5(point)-8.6(\).)-281.6(This)]TJ
-5.2638 -1.0527 TD
[(occ)-8.8(ured)-187.1(before)-190.7(a)-183.3(signi)-9.8(ficant)-193.8(bone)-185.6(marro)-10.6(w)-181.1(relea)-9(se)-185.2(respon)-7.8(se)]TJ
0 -1.0471 TD
[(was)-229.7(induce)-7.8(d)-222.7(b)0(y)-229.2(the)-227(infection)-232.7([19,)-226.2(24].)-226.2(At)-229.8(this)-230.9(time)-227.6(point)-230.5(it)]TJ
0 -1.0528 TD
[(was)-337.9(assume)-10.9(d)-330.8(that)-332.6(G-CSF)-342.5(is)-329.5(respon)-7.8(sible)-340.2(for)-329.8(the)-335.1(maj)-8.2(ority)]TJ
T*
[(of)-242.1(the)-238.4(marrow)-242.9(respon)-7.8(se)-236.4(\(fig)-8.6(s.)-235.6(1)-234.1(and)-241.7(3\),)-236.8(which)-243.8(allowed)-242.5(the)]TJ
0 -1.0471 TD
[(author)-9(s)-377.5(t)0(o)-379.5(study)-383(the)-380.7(behavi)-7.8(our)-379.5(of)-378.7(PMNL)-382.2(released)-388.5(from)]TJ
0 -1.0528 TD
[(the)-340.8(bone)-333.5(marr)-9.8(ow)-335.5(by)-331.6(G-CSF)-342.5(at)-334.4(a)-336.9(site)-339.5(of)-333.2(inflamm)-12.7(ation.)]TJ
1.0015 -1.0471 TD
[(Se)15.9(ve)15.1(ra)19.2(l)-367.3(s)0(t)17.7(u)0(d)21.2(i)0(e)18.4(s)-366.2(ha)20.8(ve)-354.7(sh)14.4(ow)17.3(n)-365(t)0(h)18.8(a)0(t)-351.4(P)0(M)14.4(N)0(L)-356.7(r)0(e)19.2(l)0(e)18.5(a)0(s)14(e)0(d)-354.8(f)0(r)18(o)0(m)]TJ
-1.0015 -1.0528 TD
[(th)13.2(e)-200.3(b)0(o)15.5(n)0(e)-195.4(m)0(a)17.7(r)0(r)18(o)0(w)-198.9(b)0(y)-195(i)0(n)18.9(f)0(l)17.3(a)0(m)12(m)0(a)17.7(t)0(o)18.9(r)0(y)-196.6(s)0(t)17.7(i)0(m)15.7(u)0(l)18.9(i)-208(su)20(ch)-195.4(as)-196.6(pn)21.2(eu)15.1(mo)18.1(-)]TJ
0 -1.0471 TD
[(co)15.1(cc)14.7(al)-288.8(pn)21.2(eu)15.1(mo)18.1(ni)18.9(a)-302.8([)0(1)19.6(9],)-299.4(bacter)-9.8(aemic)-312.1(pn)-7.2(eumonia)-313.3([24])-313.5(and)]TJ
0 -1.0527 TD
[(end)-8.4(otoxaemia)-243.6([26])-239.6(prefer)-10.2(entially)-244.1(sequester)-242.1(in)-237.2(lung)-238.7(micro-)]TJ
0 -1.0471 TD
[(vessel)-10.1(s.)-303.9(The)-302.7(princ)-8.2(iple)-309.8(factors)-308.8(that)-304.1(regul)-8.2(ate)-307.1(the)-306.7(seques)-7.8(tra-)]TJ
0 -1.0527 TD
[(tion)-389.1(of)-384.4(PM)-8.4(NL)-385.2(in)-385.2(the)-386.3(lung)-386.7(are)-386(the)-392(discrepancy)-396.4(between)]TJ
0 -1.0471 TD
[(thei)-8.2(r)-372.2(size)-383(and)-378.3(the)-375(size)-377.3(of)-378.7(pulmonary)-384.3(capillary)-386.7(segments)]TJ
0 -1.0528 TD
[(and)-412.4(the)-409.1(abil)-8.2(ity)-405.4(of)-412.8(PMNL)-410.6(to)-407.9(deform)-415.3([29,)-414(32].)-408.3(Pneumo)-7.3(-)]TJ
T*
[(coc)-8.8(cal)-324.1(pneum)-7.4(onia)-324.5(prolo)-7.8(ngs)-322.2(the)-323.7(trans)-9.4(it)-325(time)-324.3(of)-327.5(PMNL)-325.3(in)]TJ
0 -1.0471 TD
[(the)-363.6(lung)-358.3(and)-361.2(causes)-366.7(sequestrat)-11.9(ion)-357.5(of)-361.6(PMNL)-359.4(in)-362.4(both)-358.2(the)]TJ
0 -1.0527 TD
[(infec)-8.6(ted)-454.6(and)-452.3(noninf)-8.5(ected)-457(regio)-8.2(ns)-452.3(of)-452.7(the)-454.6(lung)-455([19,)-453.8(32].)]TJ
0 -1.0471 TD
[(Prev)-10(ious)-251.3(studies)-257.6(have)-254.2(show)-8.2(n)-251.2(enrichment)-263(of)-247.8(n)-6.5(ewly)-254.5(releas-)]TJ
0 -1.0527 TD
[(ed)-349.1(PMNL)-348(at)-345.7(5)-342.2(and)-349.8(8)-342.2(h)-342.2(follo)-10.2(wing)-345.1(a)-342.6(focal)-350(pneum)-7.4(ococcal)]TJ
0 -1.0471 TD
[(pne)-8.4(umonia)-374.7([19].)-382.2(The)-371(presen)-8.2(t)-373(study)-377.3(show)-8.2(s)-371.8(that)-378.1(pretreat-)]TJ
0 -1.0528 TD
[(ment)-316.3(of)-316.1(animals)-316.8(wi)-7.8(th)-311.2(G-CSF)-319.8(did)-312(not)-312(change)-318.8(this)-316.3(pattern)]TJ
0 -1.0471 TD
[(\(fig)-8.6(.)-290.6(4\),)-288(sugges)-7.4(ting)-298(that)-292.7(G-CSF)-297(does)-294.9(not)-289.2(cha)-8.8(nge)-287.2(the)-295.3(phe-)]TJ
0 -1.0528 TD
[(notypi)-9.3(c)-376.8(o)0(r)-373(funct)-8.2(ional)-378.9(characteri)-11.9(stics)-381.2(respon)-7.8(sible)-374.4(for)-381.1(this)]TJ
0 -1.0471 TD
[(incre)-8.6(ase)-226.2(in)-220.2(seques)-7.8(tration)-225(o)-6.5(f)-218.6(newly)-226.8(relea)-9(sed)-220.1(PMN)-7.4(L)-221.1(i)0(n)-220.2(lung)]TJ
0 -1.0528 TD
[(mic)-8.2(rovess)-9(els.)]TJ
1.0015 -1.0527 TD
[(G-)15.7(CS)17.3(F)-239(c)0(a)14.7(u)0(s)20(e)0(d)-229.6(a)-240.2(sh)14.4(or)19.6(te)18.4(ni)13.2(ng)-229.2(of)-230.7(t)13.9(h)0(e)-235.2(t)0(r)17.3(a)0(n)20.8(s)0(i)17.7(t)-242.2(ti)16.5(me)-227(of)-230.7(PM)14.4(NL)]TJ
-1.0015 -1.0471 TD
[(th)13.2(ro)19.6(ug)15.5(h)-376.3(t)0(h)18.9(e)-376.7(ma)17.7(tu)18.8(ra)13.5(ti)16.5(on)-365.7(po)15.5(ol)-368.1(i)13.9(n)-382(t)13.9(h)0(e)-371.8(b)0(o)21.2(n)0(e)-371.8(m)0(a)17.7(r)0(r)18(o)0(w)-369.7(a)0(n)15.1(d)-376.3(an)]TJ
0 -1.0528 TD
[(ac)14.7(ce)14.7(l)13.9(e)0(r)13.5(a)0(t)18.5(e)0(d)-445.8(r)0(e)19.2(l)0(e)12.8(a)0(s)19.6(e)-456.4(of)-441.3(th)18.9(es)13.9(e)-456.4(P)11.4(MN)15.3(L)-454.4(i)0(n)18.9(t)0(o)-447.8(t)0(h)18.8(e)-456.4(ci)18.5(rc)19.2(ul)18.9(at)12.8(i)13.9(o)0(n)15.5(.)]TJ
0 -1.0471 TD
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0 -1.0528 TD
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[(et)-197.8(al)18.9(.)]TJ
/F11 1 Tf
2.1112 0 TD
[([3)19.6(8])-208(have)-220.1(shown)-219.5(that)-218.8(G-CSF)-217.3(pretr)-9.8(eatment)-225(of)-213.7(guinea)-7.8(-)]TJ
-2.1112 -1.0527 TD
[(pigs)-251.3(incre)-8.6(ases)-256.6(sequestrat)-11.9(ion)-249.4(of)-253.5(PMNL)-251.3(in)-254.3(the)-249.8(lung)-255.8(and)-253.1(ex-)]TJ
0 -1.0471 TD
[(acerbat)-10.9(es)-634.8(acute)-644.7(lung)-637.1(inju)-7.8(ry)-640.5(induced)-640.2(by)-638.9(intratra)-11.6(cheal)]TJ
0 -1.0527 TD
[(endoto)-7.4(xin,)-283.9(a)-6.9(n)0(d)-280.4(K)]TJ
6.974 0 0 6.974 127.672 405.468 Tm
0.0001 Tc
(ING)Tj
/F3 1 Tf
9.963 0 0 9.963 142.809 405.468 Tm
0 Tc
[(et)-283.1(al.)]TJ
/F11 1 Tf
2.299 0 TD
[([15])-285.1(show)-8.2(ed)-286.5(that)-287.1(pretrea)-12.1(tment)]TJ
-10.7836 -1.0471 TD
[(with)-222.3(G-CSF)-217.3(enha)-9.6(nces)-215.6(lung)-221.7(damage)-219.5(in)-214.5(tw)-7.8(o)-211.3(model)-9.7(s)-212.5(o)0(f)-219.4(non-)]TJ
0 -1.0528 TD
[(infec)-8.6(tious)-248.8(acute)-257.8(lung)-250.1(injury)57.4(.)-245.1(The)-6.8(se)-247.8(finding)-11.7(s)-246.7(are)-249.4(suppor)-7.4(ted)]TJ
T*
[(by)-297.4(clinical)-301.4(observ)-7.8(ations)-301.2(that)-298.4(G-CSF)-297(treat)-11(ment)-299.2(could)-296.8(pre-)]TJ
0 -1.0471 TD
[(cipitate)-534.7(and)-526.2(aggrava)-8.1(te)-522.2(acute)-530.9(lung)-529(injury)-528.8([39].)-524.5(G-CSF)]TJ
0 -1.0527 TD
[(causes)-366.7(a)-6.9(n)-359.3(incre)-8.6(ased)-363.6(burden)-370.8(of)-367.3(PMNL)-365.1(in)-368.1(pulmonary)-372.9(ca-)]TJ
0 -1.0471 TD
[(pillari)-11.2(es)-395.8(\(tabl)-10.6(e)-399.5(1)0(\))-395.8(and)-401(it)-398.9(is)-403.4(speculated)-407.8(that)-400.9(the)-403.4(systemi)-10.3(c)]TJ
0 -1.0527 TD
[(relea)-9(se)-549.4(of)-555.1(inflammato)-12.1(ry)-555.1(mediator)-10.7(s)-548.3(from)-555.7(the)-551.4(focus)-559(of)]TJ
0 -1.0471 TD
[(infec)-8.6(tion,)-281.3(could)-279.7(acti)-8.6(vate)-279.4(these)-281.3(younger)-286.2(PMN)-7.4(L)-278(trapped)-283.3(in)]TJ
0 -1.0528 TD
[(the)-283.9(mic)-8.2(rovascular)-288.7(b)-6.5(e)0(d)-280.8(and)-281.5(c)-6.9(ontribut)-9.1(e)-280(t)0(o)-282.7(the)-283.9(develo)-7.8(pment)]TJ
0 -1.0471 TD
[(of)-310.4(acute)-314.7(lung)-312.7(injury)-312.5(observ)-7.8(ed)-309.2(by)-308.8(other)-8.2(s)-309.2([15,)-311.6(33,)-309.2(38,)-309.2(39].)]TJ
1.0015 -1.0528 TD
[(Th)17.1(e)-308.5(u)0(s)14.4(e)-308.5(of)-299(gr)13.9(an)20.8(ul)18.9(oc)15.1(yt)18.9(e)-308.5(c)0(o)15.1(l)0(o)18.9(n)0(y)15.5(-)0(s)18.4(t)0(i)16.5(m)0(u)18.1(l)0(a)18.4(t)0(i)16.5(n)0(g)-303.1(f)0(a)19.2(c)0(t)18.5(o)0(r)-299(t)0(o)-305.5(i)0(n)18.9(-)]TJ
-1.0015 -1.0471 TD
[(cr)19.2(ea)14.7(se)-350.2(th)18.9(e)-354(n)0(u)15.5(m)0(b)18.1(e)0(r)-350.7(o)0(f)-350.2(c)0(i)18.5(r)0(c)19.2(u)0(l)13.2(a)0(t)18.4(i)0(n)18.9(g)-359.3(po)21.2(ly)18.9(mo)12.4(rp)19.6(ho)15.5(nu)21.2(cl)18.4(ea)14.7(r)-355.2(l)0(e)18.4(u)0(-)]TJ
0 -1.0528 TD
[(ko)15.5(cy)20.8(te)18.4(s)-542.6(a)0(n)20.8(d)-535.7(th)13.2(e)-536.1(o)0(u)15.5(t)13.9(co)15.1(me)-528.6(in)-533.1(ne)20.8(ut)18.9(ro)13.9(pe)20.8(ni)13.2(c)-536.1(p)0(a)20.8(t)0(i)16.5(e)0(n)15.1(t)0(s)-528.6(w)0(i)14.9(t)0(h)]TJ
T*
[(in)18.9(fe)13.5(ct)18.5(io)18.9(n)-205.6(i)0(s)-198.6(w)0(e)16.9(l)0(l)-199.8(e)0(s)19.6(t)0(a)18.4(b)0(l)13.2(i)0(s)17.7(h)0(e)20.8(d)-205.6([1)13.9(0,)-194.6(12)15.5(,)-205.2(1)0(6)21.2(])-207.2(an)15.1(d)-205.6(s)0(t)17.7(u)0(d)15.5(i)0(e)18.4(s)-206.8(ar)19.2(e)-206(o)0(n)15.5(-)]TJ
0 -1.0471 TD
[(go)15.5(in)18.8(g)-467.4(t)0(o)-459.2(d)0(e)20.8(t)0(e)12.8(r)0(m)16.5(i)0(n)18.9(e)-467.8(th)18.9(e)-467.8(e)0(f)24.9(f)0(i)17.3(c)0(a)20.4(c)0(y)-457.2(o)0(f)-464.1(g)0(r)19.6(a)0(n)20.8(u)0(l)13.2(o)0(c)20.8(y)0(t)18.9(e)-467.8(co)15.1(lo)18.8(ny)15.5(-)]TJ
0 -1.0528 TD
[(st)17.7(im)15.7(ul)18.9(at)18.5(in)13.2(g)-222.7(f)0(a)13.5(c)0(t)18.5(o)0(r)-219.4(t)13.9(re)13.5(at)18.5(me)17.7(nt)-220.1(to)-214.5(no)15.5(n-)19.6(ne)15.1(ut)18.9(ro)13.9(pe)20.8(ni)18.9(c)-228.8(p)0(a)20.8(t)0(i)16.5(e)0(n)15.1(t)0(s)-215.7([)0(8)13.9(,)]TJ
0 -1.0471 TD
[(40].)-226.2(The)-6.8(se)-225.1(beneficial)-234.2(effects)-223.9(are)-226.6(though)-7(t)-225.1(t)0(o)-225.8(result)-228.8(from)-231.3(the)]TJ
0 -1.0528 TD
[(influence)-276.2(of)-270.6(granul)-9(ocyte)-274.5(colony)-7.4(-stimulating)-277.8(facto)-8.6(r)-269.8(o)0(n)-269(the)]TJ
0 -1.0471 TD
[(product)-9.7(ion)-283.5(o)-6.5(f)-281.2(polym)-9.3(orphonu)-6.9(clear)-293.5(leukocytes)-293.6(in)-288.4(the)-289.6(bone)]TJ
0 -1.0527 TD
[(marro)-10.6(w)-249.4(and)-253.1(the)-249.8(enhancem)-10.9(ent)-249.8(of)-253.5(the)-249.8(funct)-8.2(ional)-253.7(acti)-8.6(vity)-252.5(of)]TJ
0 -1.0471 TD
[(circu)-8.6(lating)-217(polymorph)-8.3(onucle)-7.8(ar)-214.1(leukoc)-7.8(ytes.)-212.3(Wheth)-7.8(er)-214.1(these)]TJ
0 -1.0527 TD
[(benefi)-9.4(cial)-606.1(effects)-605.1(hold)-608.6(true)-610.6(for)-608.7(pati)-8.2(ents)-604.5(when)-610.6(sepsi)-9(s)]TJ
0 -1.0471 TD
[(occurs)-218.7(with)-216.6(a)-217.4(normal)-217(funct)-8.2(ioning)-219.9(bone)-214(marr)-9.8(ow)-210.3(rema)-8.6(ins)-216.4(to)]TJ
0 -1.0527 TD
[(be)-423(determin)-10.7(ed.)-417.7(The)-422.2(inabi)-9(lity)-419.9(of)-424.2(granul)-9(ocyte)-422.4(colony)-7.4(-sti-)]TJ
T*
[(mulati)-9.6(ng)-416.9(facto)-8.6(r)-417.8(t)0(o)-425(increase)-428.3(migration)-428.9(of)-418.5(yo)-7.2(unger)-421.2(poly-)]TJ
0 -1.0471 TD
[(morp)-7.8(honucl)-7.4(ear)-323.4(leukoc)-7.8(ytes)-325.7(into)-332.2(the)-323.7(infec)-8.6(tious)-328.4(site)-328.1(results)]TJ
0 -1.0528 TD
[(in)-328.3(lar)10.4(g)-6.5(e)-325.5(numbers)-330.3(of)-327.5(less)-326.9(mat)-8.2(ure)-328.6(polymorph)-8.3(onucle)-7.8(ar)-327.9(leu-)]TJ
0 -1.0471 TD
[(kocyte)-7.8(s)-411.7(i)0(n)-413.6(the)-414.8(circu)-8.6(lation.)-416.5(The)-6.8(se)-412.8(younger)-417.1(polym)-9.3(orpho-)]TJ
25.1977 66.1583 TD
[(nuc)-8.4(lear)-485.8(leukoc)-7.8(ytes)-485(preferenti)-12.7(ally)-486.2(seques)-7.8(ter)-484.7(in)-481.9(the)-483.1(lung)]TJ
0 -1.0471 TD
[(and)-401(possi)-8.6(bly)-397.3(other)-400.8(organs)-394.7(and)-395.3(subseq)-7.4(uent)-404.2(activatio)-11.9(n)-393.4(o)0(f)]TJ
0 -1.0528 TD
[(the)-505.8(polym)-9.3(orphonu)-6.9(clear)-509.8(leukoc)-7.8(ytes)-507.8(within)-505(the)-505.8(v)-6.5(ascular)]TJ
0 -1.0471 TD
[(space)-512.7(could)-513.1(potential)-11.5(ly)-504.7(be)-508.4(harm)-8.2(ful)-507(for)-511.9(the)-511.5(host.)-507.8(This)]TJ
0 -1.0528 TD
[(balanc)-8.2(e)-302.8(between)-308.8(the)-301(benefi)-9.4(cial)-304.5(effect)-295.9(of)-299(granul)-9(ocyte)-302.9(co)-7.6(l-)]TJ
0 -1.0471 TD
[(ony)-8(-stimulating)-346.1(facto)-8.6(r)-332.4(o)0(n)-337.3(the)-335.1(funct)-8.2(ional)-339(capaci)-8.6(ty)-334(of)-333.2(ma-)]TJ
0 -1.0528 TD
[(ture)-479.8(circulatin)-14.3(g)-467.4(polym)-9.3(orphonu)-6.9(clear)-475.6(leukoc)-7.8(ytes)-473.7(an)-7.6(d)-467.4(the)]TJ
0 -1.0471 TD
[(potent)-9.7(ially)-352.8(harm)-8.2(ful)-347.7(effects)-343.4(of)-350.2(large)-344.3(numbe)-7.4(rs)-345.8(of)-350.2(less)-349.7(ma-)]TJ
0 -1.0527 TD
[(ture)-223.7(polym)-9.3(orphonu)-6.9(clear)-225.2(leukoc)-7.8(ytes)-223.3(in)-220.2(the)-221.3(circu)-8.6(lation)-222.7(may)]TJ
T*
[(deter)-8.6(mine)-521.1(the)-517.2(overal)-9.4(l)-515.3(benefi)-9.4(cial)-520.8(or)-515.3(harm)-8.2(ful)-518.4(effects)-514.1(of)]TJ
0 -1.0471 TD
[(granul)-9(ocyte)-462.2(colony)-7.4(-stimu)-9.6(lating)-467.4(factor)-466.2(adminis)-9.1(tration)-464(in)]TJ
0 -1.0528 TD
[(infec)-8.6(tious)-339.8(diseases.)]TJ
/F7 1 Tf
7.97 0 0 7.97 380.523 634.904 Tm
[(Acknowled)11.4(gements.)]TJ
/F11 1 Tf
8.5358 0 TD
[(The)-422.2(authors)-414.8(want)-422.2(to)-417.9(thank)]TJ
-9.5316 -1.1239 TD
[(S.)-563.7(Greene)-563.2(for)-570.2(photograph)12.4(y)66.1(,)-568(L.)-568.6(V)106.3(erbrugt)-563.2(for)-563.1(as-)]TJ
T*
[(sistance)-449.7(with)-454.1(the)-450.3(statistical)-452.9(analysis)-450.7(and)-447.9(J.)-460.4(Hards)]TJ
T*
[(for)-499.1(immunoc)12.8(ytochemistr)9.2(y)59(.)]TJ
/F5 1 Tf
8.966 0 0 8.966 402.349 577.757 Tm
[(Refer)11.6(ences)]TJ
/F11 1 Tf
-10.3759 -1.8842 TD
[(1.)-1766.5(Malech)-328.1(BL,)-319.9(Gallin)-329.6(JI.)-326.5(Neutrophils)-329.2(in)-323.9(human)-327.9(diseases.)]TJ
/F3 1 Tf
24.672 0 TD
(N)Tj
-22.1555 -1.1128 TD
[(Engl)-332.6(J)-334.4(Med)]TJ
/F11 1 Tf
5.071 0 TD
[(1987;)-334.9(317:)-329.1(687\261694.)]TJ
-7.5875 -1.1128 TD
[(2.)-1766.5(W)70.8(eiss)-211.2(SJ.)-210.3(T)28.6(issue)-210.7(destruction)-211.9(by)-214(neutrophils.)]TJ
/F3 1 Tf
19.9678 0 TD
[(N)-206.6(Engl)-212.5(J)-214.3(Med)]TJ
/F11 1 Tf
-17.4512 -1.1128 TD
[(1989;)-334.9(320:)-329.1(365\261376.)]TJ
-2.5165 -1.1065 TD
[(3.)-1766.5(Athens)-560(JW)82.7(,)-565.7(Haab)-563.5(OP)106.6(,)-559.3(Raab)-565(SO,)]TJ
/F3 1 Tf
17.2552 0 TD
[(et)-563.9(al)]TJ
/F11 1 Tf
2.0423 0 TD
[(.)-559.3(Leukokinetic)]TJ
-16.781 -1.1128 TD
[(studies)-464.9(IV)66.3(:)-463.5(the)-468.5(total)-466(blood,)-470.6(circulating)-465.8(and)-466.2(marginated)]TJ
T*
[(granulocyte)-442(pools)-437.3(and)-440.9(the)-443.2(granulocyte)-442(turnover)-436.3(rate)-443.5(in)]TJ
0 -1.1065 TD
[(normal)-337(subjects.)]TJ
/F3 1 Tf
6.9046 0 TD
[(J)-334.4(Clin)-336.2(Invest)]TJ
/F11 1 Tf
5.4946 0 TD
[(1961;)-334.9(40:)-336(989\261995.)]TJ
-14.9157 -1.1128 TD
[(4.)-1766.5(Boggs)-382.7(DR.)-389.3(The)-381.1(kinetics)-389.5(of)-385.7(neutrophilic)-383.3(leukocytosis)-388.7(in)]TJ
2.5165 -1.1128 TD
[(health)-336.4(and)-333.4(disease.)]TJ
/F3 1 Tf
7.9542 0 TD
[(Sem)-336.7(Hematol)]TJ
/F11 1 Tf
5.7412 0 TD
[(1967;)-328.6(4:)-336.5(359\261386.)]TJ
-16.2119 -1.1128 TD
[(5.)-1766.5(Maloney)-410(MA,)-404.2(Patt)-405.8(HM.)-410.5(Granulocyte)-407.4(transit)-410.2(from)-412.8(bone)]TJ
2.5165 -1.1065 TD
[(marrow)-332.6(to)-336.5(blood.)]TJ
/F3 1 Tf
7.3599 0 TD
(Blood)Tj
/F11 1 Tf
2.7125 0 TD
[(1968;)-328.6(3l;)-338.4(195\261201.)]TJ
-12.5889 -1.1128 TD
[(6.)-1766.5(Perlino)-550.6(CA,)-547.3(Rimland)-551.3(D.)-543.1(Alcoholism,)-554.8(leukopenia)-549.1(and)]TJ
2.5165 -1.1128 TD
[(pneumococcal)-212(sepsis.)]TJ
/F3 1 Tf
8.7636 0 TD
[(Am)-208.1(Rev)-210.2(Respir)-213.6(Dis)]TJ
/F11 1 Tf
7.5938 0 TD
[(1985;)-214.8(132:)-209(757\261)]TJ
-16.3574 -1.1128 TD
(760.)Tj
-2.5165 -1.1065 TD
[(7.)-1766.5(van)-548.4(Eeden)-544.3(SF)67.1(,)-546.7(Coetzee)-546.4(AR,)-553.7(Joubert)-544.5(JR.)-551.6(Community)]TJ
2.5165 -1.1128 TD
[(acquired)-466.5(pneumonia:)-469(factors)-475.7(influencing)-469.9(intensive)-464.9(care)]TJ
T*
(admission.)Tj
/F3 1 Tf
4.6031 0 TD
[(S)-334.6(Afr)-334.3(J)-328.1(Med)]TJ
/F11 1 Tf
5.286 0 TD
[(1988;)-334.9(73:)-336(77\26181.)]TJ
-12.4056 -1.1065 TD
[(8.)-1766.5(Dale)-224.5(DC,)-224.9(Liles)-221.6(WC,)-227.2(Summer)-221.3(WR,)-227.2(Nelson)-224.9(S.)-222.3(Granulocyte)]TJ
2.5165 -1.1128 TD
[(colony)-612.4(stimulating)-616.1(factor)-601.5(-)-607.5(role)-614.4(and)-611.6(relationships)-612.8(in)]TJ
T*
[(infectious)-338.9(diseases.)]TJ
/F3 1 Tf
8.0491 0 TD
[(J)-334.4(Infect)-337.3(Dis)]TJ
/F11 1 Tf
5.052 0 TD
[(1995;)-334.9(172:)-329.1(1061\2611075.)]TJ
-15.6176 -1.1128 TD
[(9.)-1766.5(Demetri)-304.1(GD,)-293(Griffin)-291.9(JD.)-297.2(Granulocyte)-299.9(colony)-302.6(stimulating)]TJ
2.5165 -1.1065 TD
[(factor)-335.9(and)-333.4(its)-333.2(receptor)44.7(.)]TJ
/F3 1 Tf
9.4148 0 TD
(Blood)Tj
/F11 1 Tf
2.7062 0 TD
[(1991;)-334.9(78:)-329.7(2791\2612808.)]TJ
-14.6375 -1.1128 TD
[(10.)-1266.5(Lieschke)-501.8(GJ,)-505.9(Burgess)-491.1(A)73.1(W)77.1(.)-502.4(Granulocyte)-502.2(colony-stimu-)]TJ
2.5165 -1.1128 TD
[(lating)-358(factor)-354.9(and)-358.7(granulocyte-macrophage)-357.1(colony)-353.2(stimu-)]TJ
T*
[(lating)-332.7(factor)49.8(.)]TJ
/F3 1 Tf
5.4061 0 TD
[(N)-333(Engl)-332.6(J)-334.4(Med)]TJ
/F11 1 Tf
6.07 0 TD
[(1992;)-334.9(327:)-329.1(28\26135.)]TJ
-13.9926 -1.1065 TD
[(1)25.8(1)0(.)-1292.3(Rennick)-243.4(D,)-246(Y)92(ang)-244.9(G,)-246(Gemmell)-248.1(L,)-246.2(Lee)-248.1(F)71.5(.)-243.2(Control)-247.1(of)-246.6(hema-)]TJ
2.5165 -1.1128 TD
[(topoiesis)-529.5(by)-523.8(a)-524.1(bone)-522.5(marrow)-528.6(stromal)-529.8(cell)-527.1(clone:)-526.1(lipo-)]TJ
T*
[(polysaccharides)-233.3(and)-232.2(interleukin-1)-232(inducible)-229.7(production)-234.4(of)]TJ
0 -1.1065 TD
[(colony)-416.4(stimulating)-420.1(factors)-418.8(G-CSF)-414.6(and)-415.6(GM-CSF)72.1(.)]TJ
/F3 1 Tf
20.442 0 TD
(Blood)Tj
/F11 1 Tf
-20.442 -1.1128 TD
[(1987;)-334.9(69:)-329.6(682\261691.)]TJ
-2.5165 -1.1128 TD
[(12.)-1266.5(Lord)-312.2(BI,)-317.4(Bronchud)-317.5(MH,)-315.7(Owens)-314.8(S,)]TJ
/F3 1 Tf
16.9138 0 TD
[(et)-317.3(al)]TJ
/F11 1 Tf
1.7894 0 TD
[(.)-319.1(The)-311.6(kinetics)-320(of)]TJ
-16.1867 -1.1128 TD
[(human)-112.9(granulopoiesis)-123(following)-114.3(granulocyte)-119.6(colony)-112.9(stimu-)]TJ
0 -1.1065 TD
[(lating)-282.1(factor)]TJ
/F3 1 Tf
5.1089 0 TD
[(in)-279.6(vivo)]TJ
/F11 1 Tf
2.6999 0 TD
(.)Tj
/F3 1 Tf
0.5311 0 TD
[(Pr)34.3(oc)-283.3(Natl)-285.7(Acad)-285.8(Sci)-285.2(U)-281(S)-284(A)]TJ
/F11 1 Tf
10.6541 0 TD
[(1989;)-284.4(86:)]TJ
-18.994 -1.1128 TD
(9499\2619503.)Tj
-2.5165 -1.1128 TD
[(13.)-1266.5(De)-621.7(Haas)-615.3(M,)-622.7(Kerst)-618.4(JM,)-623.4(van)-617.9(der)-618.9(Schoot)-617.3(CE,)]TJ
/F3 1 Tf
22.9901 0 TD
[(et)-620.8(al)]TJ
/F11 1 Tf
2.0992 0 TD
(.)Tj
-22.5728 -1.1128 TD
[(Granulocyte)-401.1(colony)-403.8(stimulating)-407.5(factor)-399.2(administration)-406.7(to)]TJ
0 -1.1065 TD
[(normal)-394(volunteers:)-395.3(analysis)-394.6(of)-385.7(the)-392.7(immediate)-397.6(activating)]TJ
0 -1.1128 TD
[(effects)-260.5(on)-270.9(circulating)-269.8(neutrophils.)]TJ
/F3 1 Tf
13.6006 0 TD
(Blood)Tj
/F11 1 Tf
2.643 0 TD
[(1994;)-265.4(84:)-272.7(3885\261)]TJ
-16.2436 -1.1128 TD
(3894.)Tj
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T*
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