文摘

背景:在哮喘气道炎症被认为是重要但相对难以接近的研究。微创的方法获取患者的痰液无法产生自发应该提供一个有用的哮喘的临床实验的工具。方法:方法与吸入高渗盐水诱导痰修改用于17岁哮喘病人和17名正常受试者不能自发地产生痰。成功率和安全性的方法,再现性的细胞计数和细胞计数差异哮喘和正常组织检查。高渗盐溶液3 - 5%是吸入沙丁胺醇吸入后30分钟。受试者被要求每五分钟咯血痰。样本的质量得分上的插头和唾液污染的程度。插头的下呼吸道被选为总细胞数和微分细胞嗜酸性粒细胞计数和异染性的细胞(肥大细胞和嗜碱粒细胞)直接涂片。结果:足够的样本下呼吸道获得76%的第一次尝试。意味着落在一秒用力呼气量(FEV1)在吸入生理盐水为5.3%,最大下降20%。 Eosinophil and metachromatic cell counts were reproducible (reliability coefficient 0.8 and 0.7 respectively). When compared with sputum from normal subjects sputum from asthmatic patients contained a significantly higher proportion of eosinophils (mean 18.5% (SE 3.8%) v 1.9% (0.6%)) and metachromatic cells (0.50% (0.18%) v 0.039% (0.014%)). In the asthmatic group the differential eosinophil count correlated with the baseline FEV1. CONCLUSION: Induced sputum is capable of detecting differences in cell counts between normal and asthmatic subjects and merits further development as a potential means of assessing airway inflammation in asthma.