开放病毒学杂志




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ISSN：1874-3579 - 第15卷，2021年

The Insertion of Fluorescent Proteins in a Variable Region of Respiratory Syncytial Virus L Polymerase Results in Fluorescent and Functional Enzymes But with Reduced Activities



Jenna Fix ，，，， 玛丽·加鲁克斯（Marie Galloux） ，，，， Marie-Lise Blondot ，，，， Jean-FrançoisEléouët*
INRA, Unité de Virologie Immunologie Moléculaires UR892, F-78350 Jouy-en-Josas, France

抽象的

呼吸综合病毒（RSV）大蛋白L是RNA依赖性RNA聚合酶复合物的催化亚基。当前，RSV L. RSV的L蛋白的序列比对尚无RSV的结构信息，揭示了存在两个可变区域VR1和VR2的存在。在与Morbillivirus和Rhabdovirus L基因进行比较之后，位于域V和VI之间的VR2被选为编码表位标签HA或荧光蛋白EGFP和MCHERRY的序列的插入位点。重组标记的L蛋白与转染细胞中的RSV N和P蛋白共定位。这些重组聚合酶使用病毒微型系统测定法显示出功能性，与未修饰的L聚合酶相比，它们的活性降低了约70％。我们还通过位置定向诱变表明，GDNQ基序（对于HRSV的长应变为810-813）对于L活性至关重要。

Keywords:Respiratory syncytial virus, L protein, RNA-dependent RNA polymerase, HA tag, eGFP, mCherry..

Article Information


标识符和分页：

年：2011
Volume:5
第一页：103
Last Page:108
发布者ID： TOVJ-5-103
DOI: 10.2174/1874357901101010103

文章历史：

Received Date:16/5/2011
收到的修订日期：29/6/2011
接受日期：13/7/2011
电子出版日期： 2011年6月9日
Collection year: 2011

© Fixet al.; LicenseeBentham Open

开放访问许可证：This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http：//creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.


*Address correspondence to this author at the INRA, Unité de Virologie Immunologie Moléculaires UR892, F-78350 Jouy-en-Josas, France; Tel: (33) 1 34 65 26 04; Fax: (33) 1 34 65 26 21; E-mail:jean-francois.eleouet@jouy.inra.fr


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