Ty-Jour T1 - 转录组分析显示，严重哮喘的苦味受体的上调JF - 欧洲呼吸杂志Jo - Eur Respir J SP - 65 LP - 78 Do - 10.1183 / 09031936.0007712 VL - 42是 - 1 Au - Ou - Orsmark-Pietras，ChristinaAna Au - Konradsen，Jon R. Au - Nordlund，BjörnAu--Söderhäll，Cilla Au - Pulkkinen，Ville Au - Pedroletti，Christophe Au - Daham，Kameran Au - Kupczyk，Maciek Au - Dahlén，Barbro Au - Kere，Juha Au - Dahlén，Sven-Erik Au - Hedlin，Gunilla Au - Melén，erik y1 - 2013/07/01 Ur - //www.qdcxjkg.com/content/42/1/65.abstract n2 - the严重儿童哮喘的原因很差。我们的目标是定义具有严重治疗和控制哮喘的儿童的全球基因表达模式。分离白细胞，并使用严重治疗哮喘（n = 17）的儿童中的Affymetrix人类Gene ST 1.0芯片表征了全局转录组曲线，受控哮喘（n = 19）和健康对照（n = 18）。在来自哮喘成人的分离的白细胞级分中研究了受体表达（n = 12）。总体而言，1378个基因在具有严重/控制的哮喘和对照的儿童之间差异表达。三种显着富集的基因和基因组型途径的京都植物途径：自然杀手细胞介导的细胞毒性（在受控哮喘上上调）;N-聚糖生物合成（在严重哮喘下下调）;和苦味转导受体（TAS2R）（在严重的哮喘上上调）。 Quantitative PCR experiments confirmed upregulation of TAS2Rs in severe asthmatics. TAS2R expression was replicated in leukocytes from adult asthmatics, in which TAS2R agonists also inhibited LPS-induced cytokine release. Significant correlations between expression of TAS2Rs and clinical markers of asthma severity were found in both adults and children. In conclusion, specific gene expression patterns were observed in children with severe, therapy-resistant asthma. The increased expression of bronchodilatory TAS2Rs suggests a new target for the treatment of asthma. ER -