RT期刊文章SR电子快速diagnosi T1s of primary ciliary dyskinesia: cell culture and soft computing analysis JF European Respiratory Journal JO Eur Respir J FD European Respiratory Society SP 960 OP 965 DO 10.1183/09031936.00039412 VO 41 IS 4 A1 Pifferi, Massimo A1 Bush, Andrew A1 Montemurro, Francesca A1 Pioggia, Giovanni A1 Piras, Martina A1 Tartarisco, Gennaro A1 Di Cicco, Maria A1 Chinellato, Iolanda A1 Cangiotti, Angela M. A1 Boner, Attilio L. YR 2013 UL //www.qdcxjkg.com/content/41/4/960.abstract AB Diagnosis of primary ciliary dyskinesia (PCD) sometimes requires repeated nasal brushing to exclude secondary ciliary alterations. Our aim was to evaluate whether the use of a new method of nasal epithelial cell culture can speed PCD diagnosis in doubtful cases and to identify which are the most informative parameters by means of a multilayer artificial neural network (ANN). A cross-sectional study was performed in patients with suspected PCD. All patients underwent nasal brushing for ciliary motion analysis, ultrastructural assessment and evaluation of ciliary function after ciliogenesis in culture by ANN. 151 subjects were studied. A diagnostic suspension cell culture was obtained in 117 nasal brushings. A diagnosis of PCD was made in 36 subjects (29 of whom were children). In nine out of the 36 patients the diagnosis was made only after a second brushing, because of equivocal results of both tests at first examination. In each of these subjects diagnosis of PCD was confirmed by cell culture results. Cell culture in suspension evaluated by means of ANN allows the separation of PCD from secondary ciliary dyskinesia patients after only 5 days of culture and allows diagnosis to be reached in doubtful cases, thus avoiding the necessity of a second sample.