@article {Rose909作者={玫瑰,Michala诉Kimaro, Godfather and Kroidl, Inge and Hoelscher, Michael and Bygbjerg, Ib C. and Mfinanga, Sayoki M. and Ravn, Pernille}, title = {Evaluation of QuantiFERON microtube, using 0.9 mL blood, for diagnosing tuberculosis infection}, volume = {41}, number = {4}, pages = {909--916}, year = {2013}, doi = {10.1183/09031936.00194311}, publisher = {European Respiratory Society}, abstract = {The performance of QuantiFERON microtube (QFT-MT), using 0.9 mL blood, and QuantiFERON-TB Gold in-tube test (QFT-IT) (3 mL blood), for diagnosing tuberculosis (TB) was compared in children and adults in an endemic setting. In 152 children with suspected TB and 87 adults with confirmed TB, QFT-IT was compared with two QFT-MT concentrations (QFT-MT A and B). Proportions of positive and indeterminate results, interferon (IFN)-γ responses, interassay agreement and sensitivity were assessed. We found similar proportions of indeterminate results, levels of IFN-γ and comparable sensitivity. The interassay agreement was moderate in all children (QFT-IT versus QFT-MT A: 85\%, k=0.44 and QFT-IT versus QFT-MT B: 88\%, k=0.50) and adults (QFT-IT versus QFT-MT A: 88\%, k=0.50 and QFT-IT versus QFT-MT B: 89\%, k=0.49). Sensitivity was low (QFT-IT 23\%, QFT-MT A 18\% and B 19\%) in children with confirmed or highly probable TB compared with adults (83\%, 86\% and 88\%, respectively). The QFT-MT test can be reliably performed using less than one-third of the blood volume used in QFT-IT. The reduced volume may be useful for research and future diagnosis of paediatric TB. The poor sensitivity and high indeterminate rate of both IFN-γ release assays in severely ill children, with immature or impaired immunity in an endemic setting, warrants further investigations.}, issn = {0903-1936}, URL = {//www.qdcxjkg.com/content/41/4/909}, eprint = {//www.qdcxjkg.com/content/41/4/909.full.pdf}, journal = {European Respiratory Journal} }