TY - T1的转录组测序(RNA-Seq)人类支气管活检:哮喘< em >和< / em >控件JF -欧洲呼吸杂志》乔和J SP - 662 LP - 670欧元——10.1183/09031936.00115412六世- 42 - 3 AU - Ching勇活跃盟Aeilko h . Zwinderman AU -彼得·w·Kunst盟Katrien格伦伯格AU -泰国人Mauad盟Annemiek Dijkhuis AU -伊丽莎白h·贝尔盟-弗兰克先生盟Rene附近地区盟-彼得·J·斯德克已Y1 - 2013/09/01 UR - //www.qdcxjkg.com/content/42/3/662.abstract N2 -哮喘的细胞和分子途径是高度复杂的。增加理解可以得到公正的转录组分析(RNA-Seq)。我们提出,整个人类支气管活检的转录组剖面不同哮喘患者和控制。首先,我们调查的可行性获得RNA从支气管活检适合RNA-Seq整体。其次,我们对转录组配置文件之间的差异哮喘和控制。这个横断面研究比较四个前者过敏性哮喘患者和5个健康nonatopic控制。总RNA从四个每个主题是准备RNA-Seq活检。比较数字的读每个基因在哮喘和控制是基于泊松分布。46个基因之间的差异表达哮喘和控制,包括pendrin periostin BCL2。10基因网络被发现参与细胞形态、运动和发展。 RNA isolated from whole human endobronchial biopsies is suitable for RNA-Seq, showing different transcriptomic profiles between asthma and controls. Novel and confirmative genes were found to be linked to asthma. These results indicate that biological processes in the airways of asthma patients are regulated differently when compared to controls, which may be relevant for the pathogenesis and treatment of the disease. Transcriptome sequencing shows processes in the airways of asthmatics are differently regulated at transcriptomic level http://ow.ly/kDnln ER -