TY - JOUR T1 -基因型MTBDRsl对具有不同遗传背景的临床样本的表现JF -欧洲呼吸杂志JO - Eur Respir J SP - 690 LP - 698 DO - 10.1183/09031936.00164111 VL - 40 IS - 3 AU - Miotto, Paolo AU - Cabibbe, Andrea M. AU - Mantegani, Paola AU - Borroni, Emanuele AU - Fattorini, Lanfranco AU - Tortoli, Enrico AU - Migliori, Giovanni B. AU - Cirillo，Daniela M. Y1 - 2012/09/01 UR - //www.qdcxjkg.com/content/40/3/690.abstract N2 -我们评估了gengentype®MTBDRsl (Hain Lifescience Nehren, Germany)用于二线耐药结核病检测的性能，并将不同结核分枝杆菌基因型的突变频率关联起来。我们测试了175株菌株和59个临床标本，根据诊断准确性报告标准的建议解释结果。对所有菌株进行了spoligotyping和mycobacteria Interspersed repetition Units-Variable Number of Tandem Repeats分型。MTBDRsl检测临床分离株对氟喹诺酮类药物(FQ)、二线注射药物(SLID)和乙胺丁醇(EMB)耐药的表现相似(特异性~ 99%，敏感性~ 70%，阳性预测值(PPV) ~ 99%)。在59份呼吸道标本中，有3份样本被归类为“不确定”。FQs和EMB检测耐药性的特异性相似，分别为100% (95% CI 92.7-100%)和100% (95% CI 83.9-100%)， PPV分别为100% (95% CI 64.6-100%)和100% (95% CI 87.9-100%)。slide检测特异性为89.1% (95% CI 77.0 ~ 95.3%)， PPV为58.3% (95% CI 32.0 ~ 80.7%)。fq -耐药检测的敏感性为100% (95% CI 64.6-100%)，而对SLID和EMB的敏感性分别为89.1% (95% CI 77.0-95.3%)和86.1% (95% CI 71.3-93.9%)。我们检测到rrs基因突变与北京血统之间的显著关联。 The MTBDRsl can be used to “rule in” extensively drug-resistant strains of tuberculosis in a high risk group; the low sensitivity and negative predicted value (NPV) make confirmation by conventional drug susceptibility testing mandatory when mutations are not identified. NPV for SLID is higher in Beijing strains, showing that the predictive values of the molecular tests are related to the genetic background. ER -