Ty- Jour T1-支气管下皮下肌纤维细胞的细胞培养物增强了体外JF的嗜酸性粒细胞生存期 - 欧洲呼吸杂志JO -EUR RESSIR J SP -1839 LP -1846 LP -1846 VL -9 IS -9 AU -9 AU -ZHANG -ZHANG，SAU -MOHAMM -MOHAMM- MOHAMM，Q AU-Burbidge，Au -Morland，CM Au -Roche，WR Y1-1996/09/01 UR -http：//www.qdcxjkg.com/content/9/9/9/1839.Abstract.Abstract N2-嗜酸性粒细胞积累和激活机制支气管粘膜对于哮喘的发病机理至关重要。支气管基底膜下方的专门成纤维细胞，肌纤维细胞的位置及其浸入嗜酸性粒细胞的近端有可能使肌纤维细胞调节嗜酸性粒细胞在哮喘中的生存和功能。这项研究的目的是研究支气管肌纤维细胞对体外嗜酸性粒细胞存活的影响。来自人类外周血的嗜酸性粒细胞暴露于支气管肌纤维细胞和肌成纤维细胞条件培养基中。评估了嗜酸性粒细胞活力，并在共培养上清液中检查了粒细胞/巨噬细胞刺激因子（GM-CSF）的产生，并作为肌纤维细胞中的允许核糖核酸（mRNA）。嗜酸性粒细胞的存活显着增加，嗜酸性粒细胞凋亡通过与肌纤维细胞共培养抑制。来自肿瘤坏死因子-Alpha（TNF-Alpha）刺激的肌纤维细胞的条件培养基也延长了嗜酸性粒细胞的存活率。GM-CSF抗体可以阻止这种作用。 GM-CSF mRNA and secretion from myofibroblasts were increased in co-cultures and by eosinophil-conditioned medium. Addition of antibodies to TNF-alpha and interleukin-1 alpha (IL-1 alpha) to co-cultures resulted in significant reduction both in eosinophil survival and GM-CSF levels. Blocking of fibronectin in the co-cultures did not affect the eosinophil survival enhancing activity. Prednisolone inhibited the eosinophil survival enhancing activity of the co-cultures by suppression of GM-CSF production. Soluble eosinophil-derived cytokines are involved in the interaction of eosinophils with myofibroblasts, which results in a tumour necrosis factor-alpha/interleukin-1 alpha mediated release of granulocyte/macrophage colony-stimulating factor from myofibroblasts. Bronchial myofibroblasts can, thereby, contribute to allergic inflammation by granulocyte/macrophage colony-stimulating factor-mediated inhibition of eosinophil apoptosis. ER -