Ty-jour t1 - 来自支气管上皮细胞染色体纤维素细胞增强嗜酸性粒细胞存活体外jf - 欧洲呼吸杂志jo - 欧元jo - eur reshir J sp - 1839 Lp - 1846 VL - 9是 - 9互张，S au - 穆罕默德，Q Au -Burbidge，A Au - Morland，CM Au - Roche，WR Y1 - 1996/09/01 UR - //www.qdcxjkg.com/content/9/9/1839.abstract n2 - 嗜酸性粒细胞累积和激活机制支气管粘膜对于哮喘发病机制至关重要。在支气管基底膜下方的专用成纤维细胞，肌成纤维细胞，近距离渗透嗜酸性粒细胞的位置可能使肌纤维细胞能够调节嗜酸性粒细胞存活和在哮喘中的功能。本研究的目的是探讨支气管肌纤维素对体外嗜酸性粒细胞存活的影响。来自人外周血的嗜酸性粒细胞暴露于来自支气管肌纤维素细胞和肌纤维细胞调节培养基的细胞培养物。评估嗜酸性粒细胞活力，并在共培养上清液中检测粒细胞/巨噬细胞菌刺激因子（GM-CSF）产生，并在肌纤维细胞中的信使核糖核酸（mRNA）中。嗜酸性粒细胞存活率显着增加，嗜酸性粒细胞凋亡被肌纤维细胞共培养抑制。来自肿瘤坏死因子-α（TNF-α）的条件培养基（TNF-α） - 刺激的肌纤维细胞也延长了嗜酸性粒细胞存活。通过GM-CSF抗体可以阻断这种效果。 GM-CSF mRNA and secretion from myofibroblasts were increased in co-cultures and by eosinophil-conditioned medium. Addition of antibodies to TNF-alpha and interleukin-1 alpha (IL-1 alpha) to co-cultures resulted in significant reduction both in eosinophil survival and GM-CSF levels. Blocking of fibronectin in the co-cultures did not affect the eosinophil survival enhancing activity. Prednisolone inhibited the eosinophil survival enhancing activity of the co-cultures by suppression of GM-CSF production. Soluble eosinophil-derived cytokines are involved in the interaction of eosinophils with myofibroblasts, which results in a tumour necrosis factor-alpha/interleukin-1 alpha mediated release of granulocyte/macrophage colony-stimulating factor from myofibroblasts. Bronchial myofibroblasts can, thereby, contribute to allergic inflammation by granulocyte/macrophage colony-stimulating factor-mediated inhibition of eosinophil apoptosis. ER -