ty -jour t1-肺癌细胞系的ICAM -1表达：细胞因子上调上调的影响对LAK细胞的相互作用JF-欧洲呼吸杂志JO -EUR RESSIR J SP -1831 LP -1838 VL -1838 VL -9 IS -9 AU -9 AU-梅利斯（Melis），M au -spatafora，m au -melodia，au -pace，e au -au -gjomarkaj，m au -merendino，am au -au -bonsignore，g y1-1996/09/01 ur -http：//erj.ersjournals。com/content/9/9/1831.Abstract N2-肿瘤细胞的细胞间粘附分子1（ICAM-1）表达可能与它们与防御细胞的相互作用有关。在这项研究中，研究了五个小细胞肺癌（SCLC）和五个非SCLC（NSCLC）细胞系的表面ICAM-1表达和可溶性ICAM-1（SICAM-1）。此外，还评估了细胞因子上调ICAM-1上调对肺癌细胞对同种异体淋巴细胞激活杀伤（LAK）细胞粘附的粘附以及对LAK细胞毒性的敏感性。通过流式细胞仪评估ICAM-1表达。可溶性ICAM-1释放是通过酶联免疫吸附测定（ELISA）测量的。通过粘附和细胞毒性测定测试与LAK细胞的相互作用。在基线时，SCLC线未表达ICAM-1，而5个NSCLC线中的4条表示ICAM-1。ICAM-1的表达是通过5个SCLC线中的4种干扰素γ（IFN-GAMMA）诱导的，并在5个NSCLC线中的1个上调。 ICAM-1 expression was induced by tumour necrosis factor-alpha (TNF-alpha) in 1 of the 5 SCLC lines (National Cancer Institute (NCI) H211), and upregulated in 2 of the 5 NSCLC lines (NCI H460 and NCI H838). Among the latter lines, one (NCI H838) released significant amounts of sICAM-1. Adhesion to LAK cells and susceptibility to LAK cytotoxicity were significantly higher in TNF-alpha-treated NCI H460 and NCI H211 cells, compared to untreated NCI H460 and NCI H211 cells. In contrast, no difference in adhesion to LAK cells and susceptibility to LAK cytotoxicity was detected between baseline and TNF-alpha-treated NCI H838 cells. Intercellular adhesion molecule-1 surface expression and soluble intercellular adhesion molecule-1 release may play an important role in interactions between lymphokine-activated killer cells and lung cancer cells. ER -